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26  structures 96  species 3  interactions 104  sequences 3  architectures

Family: MLANA (PF14991)

Summary: Protein melan-A

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This is the Wikipedia entry entitled "MLANA". More...

MLANA Edit Wikipedia article

PDB 2GUO - MHC HLA-A2 in complex with Melan-A-MART-1 27-35 peptide.png
Available structures
PDB Ortholog search: PDBe RCSB
Aliases MLANA, MART-1, MART1, melan-A
External IDs MGI: 108454 HomoloGene: 4026 GeneCards: MLANA
Gene location (Human)
Chromosome 9 (human)
Chr. Chromosome 9 (human)[1]
Chromosome 9 (human)
Genomic location for MLANA
Genomic location for MLANA
Band 9p24.1 Start 5,890,802 bp[1]
End 5,910,606 bp[1]
RNA expression pattern
PBB GE MLANA 206426 at fs.png

PBB GE MLANA 206427 s at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 9: 5.89 – 5.91 Mb Chr 19: 29.7 – 29.71 Mb
PubMed search [3] [4]
View/Edit Human View/Edit Mouse

Protein melan-A also known as melanoma antigen recognized by T cells 1 or MART-1 is a protein that in humans is encoded by the MLANA o "MALENA" gene.[5] A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines.

Discovery and nomenclature

The names MART-1 and melan-A were coined by two groups of researchers who independently sequenced the gene for this antigen in 1994. Both names are currently in common use. Kawakami et al. at the National Cancer Institute coined the term MART-1, which stands for "melanoma antigen recognized by T-cells."[6] Coulie et al. of Belgium called the gene melan-A, presumably an abbreviation for "melanocyte antigen."[7]

Clinical significance

MART-1/melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The same name is also used to refer to the gene which codes for the antigen.

The MART-1/melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well.

In many immunological studies melan-A peptides serve as a positive control for T-cell priming experiments. This is due to the fact that its high precursor frequency of about 1/1000 among cytotoxic T-cells makes it easy for antigen presenting cells to evoke peptide-specific responses.[8]


MART-1/melan-A is a putative 18 kDa transmembrane protein consisting of 118 amino acids. It has a single transmembrane domain.


Its expression is regulated by the Microphthalmia-associated transcription factor.[9][10]


  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000120215 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024806 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ "Entrez Gene: MLANA melan-A". 
  6. ^ Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Rivoltini L, Topalian SL, Miki T, Rosenberg SA (April 1994). "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor". Proc. Natl. Acad. Sci. U.S.A. 91 (9): 3515–9. doi:10.1073/pnas.91.9.3515. PMC 43610Freely accessible. PMID 8170938. 
  7. ^ Coulie PG, Brichard V, Van Pel A, Wölfel T, Schneider J, Traversari C, Mattei S, De Plaen E, Lurquin C, Szikora JP, Renauld JC, Boon T (July 1994). "A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas". J. Exp. Med. 180 (1): 35–42. doi:10.1084/jem.180.1.35. PMC 2191574Freely accessible. PMID 8006593. 
  8. ^ Zippelius A, Pittet MJ, Batard P, et al. (2002). "Thymic selection generates a large T cell pool recognizing a self-peptide in humans". J. Exp. Med. 195 (4): 485–94. doi:10.1084/jem.20011658. PMC 2193620Freely accessible. PMID 11854361. 
  9. ^ Du J, Miller AJ, Widlund HR, Horstmann MA, Ramaswamy S, Fisher DE (2003). "MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma". Am. J. Pathol. 163 (1): 333–43. doi:10.1016/S0002-9440(10)63657-7. PMC 1868174Freely accessible. PMID 12819038. 
  10. ^ Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. 

Further reading

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Protein melan-A Provide feedback

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Literature references

  1. Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Rivoltini L, Topalian SL, Miki T, Rosenberg SA;, Proc Natl Acad Sci U S A. 1994;91:3515-3519.: Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor. PUBMED:8170938 EPMC:8170938

  2. Coulie PG, Brichard V, Van Pel A, Wolfel T, Schneider J, Traversari C, Mattei S, De Plaen E, Lurquin C, Szikora JP, Renauld JC, Boon T;, J Exp Med. 1994;180:35-42.: A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. PUBMED:8006593 EPMC:8006593

  3. Giordano F, Bonetti C, Surace EM, Marigo V, Raposo G;, Hum Mol Genet. 2009;18:4530-4545.: The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition. PUBMED:19717472 EPMC:19717472

This tab holds annotation information from the InterPro database.

InterPro entry IPR029242

Melan-A, also known as melanoma antigen recognised by T-cells 1, or MART-1, is a protein that in humans is encoded by the MLANA gene. It is required for the function of the melanosomal matrix protein PMEL17/GP100 and thus plays an important role in regulating mammalian pigmentation [PUBMED:15695812]. Melan-A is a member of the MAGE gene family [PUBMED:11454705] and is a tumour associated antigen. Clinically, the protein may serve as an anti-cancer vaccine [PUBMED:24045055].

Gene Ontology

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Domain organisation

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Seed source: Jackhmmer:Q16655
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 19
Number in full: 104
Average length of the domain: 105.00 aa
Average identity of full alignment: 48 %
Average coverage of the sequence by the domain: 85.46 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.2 31.8
Noise cut-off 24.7 23.2
Model length: 117
Family (HMM) version: 6
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Species distribution

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There are 3 interactions for this family. More...

MHC_I MHC_II_alpha MHC_II_beta


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MLANA domain has been found. There are 26 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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