Summary: Something about silencing, SAS, complex subunit 4
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Something about silencing, SAS, complex subunit 4 Provide feedback
SAS4 is a family of largely fungal silencing regulators. This silencing is mediated by chromatin. SAS4 specifically silences the yeast mating-type genes HML and HMR [1]. SAS4 is found to be one subunit of a complex, the SAS complex, that interacts with chromatin assembly factor Asf1p, and asf1 mutants show silencing defects similar to mutants in the SAS complex. Thus, ASF1-dependent chromatin-assembly may mediate the role of the SAS complex in silencing [2]. Co-expression of Sas2, SAS4, and Sas5 in Escherichia coli leads to formation of a stable SAS complex that acetylates histones. SAS4 is essential for the acetyltransferase activity of Sas2, and Sas5 is also important [3].
Literature references
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Xu EY, Kim S, Replogle K, Rine J, Rivier DH;, Genetics. 1999;153:13-23.: Identification of SAS4 and SAS5, two genes that regulate silencing in Saccharomyces cerevisiae. PUBMED:10471696 EPMC:10471696
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Osada S, Sutton A, Muster N, Brown CE, Yates JR 3rd, Sternglanz R, Workman JL;, Genes Dev. 2001;15:3155-3168.: The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. PUBMED:11731479 EPMC:11731479
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Sutton A, Shia WJ, Band D, Kaufman PD, Osada S, Workman JL, Sternglanz R;, J Biol Chem. 2003;278:16887-16892.: Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex. PUBMED:12626510 EPMC:12626510
Internal database links
SCOOP: | PEHE |
This tab holds annotation information from the InterPro database.
InterPro entry IPR029184
SAS4 is a fungal silencing regulator. In S. cerevisiae, Sas4 is a subunit of a histone acetyltransferase complex termed SAS complex, which consists of Sas2, Sas4 and Sas5 [PUBMED:15659401]. The SAS complex acetylates both free histones and nucleosomes and is involved in transcriptional silencing. Both Sas4 and Sas5 are required for optimal Sas2 histone acetyltransferase activity [PUBMED:12626510].
This entry represents a domain found in Sas4 (something about silencing protein 4).
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Biological process | histone acetylation (GO:0016573) |
Domain organisation
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Alignments
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Seed (61) |
Full (390) |
Representative proteomes | UniProt (550) |
NCBI (590) |
Meta (0) |
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RP15 (68) |
RP35 (188) |
RP55 (283) |
RP75 (395) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
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Seed (61) |
Full (390) |
Representative proteomes | UniProt (550) |
NCBI (590) |
Meta (0) |
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RP15 (68) |
RP35 (188) |
RP55 (283) |
RP75 (395) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
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Curation and family details
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Curation
Seed source: | Jackhmmer:Q04003 |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Coggill P |
Number in seed: | 61 |
Number in full: | 390 |
Average length of the domain: | 96.50 aa |
Average identity of full alignment: | 42 % |
Average coverage of the sequence by the domain: | 17.27 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 99 | ||||||||||||
Family (HMM) version: | 6 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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