Summary: Something about silencing, SAS, complex subunit 4
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SAS4 is a family of largely fungal silencing regulators. This silencing is mediated by chromatin. SAS4 specifically silences the yeast mating-type genes HML and HMR . SAS4 is found to be one subunit of a complex, the SAS complex, that interacts with chromatin assembly factor Asf1p, and asf1 mutants show silencing defects similar to mutants in the SAS complex. Thus, ASF1-dependent chromatin-assembly may mediate the role of the SAS complex in silencing . Co-expression of Sas2, SAS4, and Sas5 in Escherichia coli leads to formation of a stable SAS complex that acetylates histones. SAS4 is essential for the acetyltransferase activity of Sas2, and Sas5 is also important .
Osada S, Sutton A, Muster N, Brown CE, Yates JR 3rd, Sternglanz R, Workman JL;, Genes Dev. 2001;15:3155-3168.: The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. PUBMED:11731479 EPMC:11731479
Sutton A, Shia WJ, Band D, Kaufman PD, Osada S, Workman JL, Sternglanz R;, J Biol Chem. 2003;278:16887-16892.: Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex. PUBMED:12626510 EPMC:12626510
Internal database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR029184
This entry represents a domain found in Sas4 (something about silencing protein 4).
SAS4 is a fungal silencing regulator. In S. cerevisiae, Sas4 is a subunit of a histone acetyltransferase complex termed SAS complex, which consist of Sas2, Sas4 and Sas5 [PUBMED:15659401]. The SAS complex acetylates both free histones and nucleosomes and is involved in transcriptional silencing. Both Sas4 and Sas5 are required for optimal Sas2 histone acetyltransferase activity [PUBMED:12626510].
|Biological process||histone acetylation (GO:0016573)|
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|Number in seed:||63|
|Number in full:||311|
|Average length of the domain:||94.80 aa|
|Average identity of full alignment:||42 %|
|Average coverage of the sequence by the domain:||17.21 %|
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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||5|
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