# STOCKHOLM 1.0 #=GF ID SAS4 #=GF AC PF15460.6 #=GF DE Something about silencing, SAS, complex subunit 4 #=GF AU Coggill P;0000-0001-5731-1588 #=GF SE Jackhmmer:Q04003 #=GF GA 24.40 24.40; #=GF TC 24.40 24.50; #=GF NC 24.30 24.30; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 10471696 #=GF RT Identification of SAS4 and SAS5, two genes that regulate #=GF RT silencing in Saccharomyces cerevisiae. #=GF RA Xu EY, Kim S, Replogle K, Rine J, Rivier DH; #=GF RL Genetics. 1999;153:13-23. #=GF RN [2] #=GF RM 11731479 #=GF RT The yeast SAS (something about silencing) protein complex #=GF RT contains a MYST-type putative acetyltransferase and functions #=GF RT with chromatin assembly factor ASF1. #=GF RA Osada S, Sutton A, Muster N, Brown CE, Yates JR 3rd, Sternglanz #=GF RA R, Workman JL; #=GF RL Genes Dev. 2001;15:3155-3168. #=GF RN [3] #=GF RM 12626510 #=GF RT Sas4 and Sas5 are required for the histone acetyltransferase #=GF RT activity of Sas2 in the SAS complex. #=GF RA Sutton A, Shia WJ, Band D, Kaufman PD, Osada S, Workman JL, #=GF RA Sternglanz R; #=GF RL J Biol Chem. 2003;278:16887-16892. #=GF DR INTERPRO; IPR029184; #=GF DR SO; 0100021; polypeptide_conserved_region; #=GF CC SAS4 is a family of largely fungal silencing regulators. This #=GF CC silencing is mediated by chromatin. SAS4 specifically silences #=GF CC the yeast mating-type genes HML and HMR [1]. SAS4 is found to be #=GF CC one subunit of a complex, the SAS complex, that interacts with #=GF CC chromatin assembly factor Asf1p, and asf1 mutants show silencing #=GF CC defects similar to mutants in the SAS complex. Thus, #=GF CC ASF1-dependent chromatin-assembly may mediate the role of the #=GF CC SAS complex in silencing [2]. Co-expression of Sas2, SAS4, and #=GF CC Sas5 in Escherichia coli leads to formation of a stable SAS #=GF CC complex that acetylates histones. SAS4 is essential for the #=GF CC acetyltransferase activity of Sas2, and Sas5 is also important #=GF CC [3]. #=GF SQ 61 #=GS D4AQR4_ARTBC/319-415 AC D4AQR4.1 #=GS C0NG53_AJECG/422-518 AC C0NG53.1 #=GS U1HJ68_ENDPU/226-322 AC U1HJ68.1 #=GS H6C7D1_EXODN/293-389 AC H6C7D1.1 #=GS K2SIA9_MACPH/286-382 AC K2SIA9.1 #=GS M2MVB7_BAUCO/274-370 AC M2MVB7.1 #=GS N1PN60_DOTSN/305-401 AC N1PN60.1 #=GS N1QIB8_SPHMS/287-383 AC N1QIB8.1 #=GS M2SL66_COCSN/288-384 AC M2SL66.1 #=GS S8AHC8_DACHA/482-581 AC S8AHC8.1 #=GS L0PB25_PNEJ8/139-235 AC L0PB25.1 #=GS M7PLS0_PNEMU/159-255 AC M7PLS0.1 #=GS Q6CCM5_YARLI/191-289 AC Q6CCM5.1 #=GS W1Q9C3_OGAPD/96-199 AC W1Q9C3.1 #=GS C4QY52_KOMPG/87-198 AC C4QY52.1 #=GS G3AX74_CANTC/88-186 AC G3AX74.1 #=GS G8YKJ9_PICSO/82-180 AC G8YKJ9.1 #=GS B5RUT9_DEBHA/85-183 AC B5RUT9.1 #=GS A3LZL5_PICST/81-178 AC A3LZL5.2 #=GS A5E7W5_LODEL/110-206 AC A5E7W5.1 #=GS C5MDI7_CANTT/119-217 AC C5MDI7.1 #=GS G8BH61_CANPC/85-183 AC G8BH61.1 #=GS C4Y5Z7_CLAL4/91-189 AC C4Y5Z7.1 #=GS A5DMH2_PICGU/189-287 AC A5DMH2.2 #=GS C5DKZ9_LACTC/98-193 AC C5DKZ9.1 #=GS S6E1I9_ZYGB2/84-180 AC S6E1I9.1 #=GS C5DSY4_ZYGRC/86-184 AC C5DSY4.1 #=GS I2H7N4_TETBL/167-265 AC I2H7N4.1 #=GS G8ZXH3_TORDC/101-195 AC G8ZXH3.1 #=GS A7TLI0_VANPO/131-229 AC A7TLI0.1 #=GS G8BRL9_TETPH/141-238 AC G8BRL9.1 #=GS H2ARA7_KAZAF/106-204 AC H2ARA7.1 #=GS J7S3R9_KAZNA/141-239 AC J7S3R9.1 #=GS SAS4_YEAST/124-221 AC Q04003.1 #=GS Q6FU72_CANGA/98-195 AC Q6FU72.1 #=GS G0W381_NAUDC/162-257 AC G0W381.1 #=GS G0VBR8_NAUCC/118-211 AC G0VBR8.1 #=GS Q6CQC2_KLULA/86-184 AC Q6CQC2.1 #=GS Q750S1_ASHGO/86-181 AC Q750S1.2 #=GS G8JTA8_ERECY/91-186 AC G8JTA8.1 #=GS K0KWZ0_WICCF/115-210 AC K0KWZ0.1 #=GS K1XZH4_MARBU/283-379 AC K1XZH4.1 #=GS G2YXH1_BOTF4/264-360 AC G2YXH1.1 #=GS S3CVW4_GLAL2/260-356 AC S3CVW4.1 #=GS L8G9Y1_PSED2/272-368 AC L8G9Y1.1 #=GS G0SGP7_CHATD/259-356 AC G0SGP7.1 #=GS C7YJ20_NECH7/266-362 AC C7YJ20.1 #=GS U7PXI7_SPOS1/495-591 AC U7PXI7.1 #=GS G2Q6V6_MYCTT/295-391 AC G2Q6V6.1 #=GS Q2HFJ0_CHAGB/261-357 AC Q2HFJ0.1 #=GS G4N6E7_MAGO7/302-398 AC G4N6E7.1 #=GS L2G7W8_COLGN/250-346 AC L2G7W8.1 #=GS A7UVZ4_NEUCR/289-385 AC A7UVZ4.1 #=GS A0A0E1RY21_COCIM/119-215 AC A0A0E1RY21.1 #=GS C4JIL8_UNCRE/78-174 AC C4JIL8.1 #=GS G7XR06_ASPKW/336-433 AC G7XR06.1 #=GS B8MXV1_ASPFN/339-436 AC B8MXV1.1 #=GS Q0D1Q9_ASPTN/340-434 AC Q0D1Q9.1 #=GS Q4WCB1_ASPFU/365-461 AC Q4WCB1.1 #=GS B6HFN3_PENRW/301-397 AC B6HFN3.1 #=GS S7ZMG8_PENO1/348-444 AC S7ZMG8.1 D4AQR4_ARTBC/319-415 DPLDEDTFFKAHRRLERQEKQLRNLEKERAQYEKTQLDHLLGEL.............QGHDWLRVMGING.VTD....SEKKLYEPKRDYFVGEVTALLEKFRAWKEEEKRRRIE C0NG53_AJECG/422-518 DPLDDDVYFKAHRRIERQEKQLRNIEKERAQHEKVQLDRLLDEL.............QGHDWLRVMGISG.ITE....TEKKLYEPKRAYFIKEVSALIEKFRLWKEEERRRKME U1HJ68_ENDPU/226-322 DTLSEDIFYRAHRKAERHEKQLRNIEKERAQHEKVQLDRLLEEL.............RGPDWLKVMGISG.VTE....SEKKLYEPKRVFFIREVAGLIEKFRHWKEEEKRRKIE H6C7D1_EXODN/293-389 DPLGEEVYSKIHSKAERHEKQMKNSDRERAQHEKYQLEKLLDDL.............RGPDWLKTLGISG.VTD....TEKRRYEPKRLLFIRETQALIDKFKRWKEEEKRRKLE K2SIA9_MACPH/286-382 DPLEDDVFFKAHRRAERKEKQLRNIEKERAMHEKVQLERLLDGL.............LGHDWLRVMGITG.ITD....SEKKEFEPKRDYFISEVRALVDKFRIWKEEEKRLRIE M2MVB7_BAUCO/274-370 DPLSDTYYGKSHRRAERREKQLRNIEKERAMHEKVQLERLLEGL.............QGHDWLKVLGITG.ITD....GEAKKYEPKRDYFIEEVKALVDKFRLWKEQERKQKME N1PN60_DOTSN/305-401 DPLSDEHFLKPHKRAERKEKQLRNIERERAMHEKVQLDKLLDGL.............QGHDWLRVLGITG.VTE....SEARRYEKKRDYFIAEVEALLDKFKQWRDQEKKQRAS N1QIB8_SPHMS/287-383 DPLTDAHFLKSHNRAERKEKQLRNIERERAMHEKVQLDRLLDGL.............QGHDWLRVLGITG.ITD....GEAKKYERKRKYFIAEVQALIGKFAQWKEQEKKLRLR M2SL66_COCSN/288-384 DPLADSVYFTQHRRAERKEKQLRNIEKERAMHEKVQLERLLDGL.............QGPDWLKVMGITG.VTD....GERKDWEAKRNYFIREVEALVDKFRVWKEEEKRLRAD S8AHC8_DACHA/482-581 DRLTDRYYLDAHKIKCAQEKADHKKELEKEQKNFNRYKQLLEQLEDV..........DGNEWMEILG....VEKPETLKEVANLLRKRDRLMDELKAARRRYPIYKAEREAIDK. L0PB25_PNEJ8/139-235 DPLPDNLFHLNHKRFDRQEKHIRNIERERFAHDQFYLEQKIDQL.............HSSDWKKIVIAISKIT.....GSKDPLETQRQFIIQKLEHVLKKFNSWKEFELKNKRK M7PLS0_PNEMU/159-255 DPLSDNLFHLNHKRFDRQEKHIRNIEYERFTYDRLYLEQKIDQL.............NGSNWKKVVMAISKIT.....GCKDPLEIQRQYIIERLEQVLKKFDSWRKLQLEHKKR Q6CCM5_YARLI/191-289 DPLPDSLYEGDHKRMERDEKRVQSTERARIMEEKDRLVTQMEQL.............ESGEWLRYIAGITKIHNM...GDKEELARKRELTLKECRFMVKSYEQFKEREKQYLAR W1Q9C3_OGAPD/96-199 DPLDSKLFEAFHKRGEKEEKRFARDDRNKLLREYQNCMELLGKL.......GGSKAEQKDDRLRFLLETTKINNV...NDPMELDIKFMLTVKELIHFVLNYERVKQLEGEIKS. C4QY52_KOMPG/87-198 DPLDDKLFLKHHQRAEKSEKKMTNIEKERVFTEYDYYLHCLEQLKTVCQDYEASMVPNQQMMLRITEPFTTIDDP...NDRQEVLMKIQLLIGEITKFTRTFERLRKLELKLKTL G3AX74_CANTC/88-186 DPLSDDKYLPFHRVMTKRETSMNNLDKSKILSEMDGLQTQKSQL.............LNNNWASVLPTITRINDP...GDENELIEKRKLTVAEIDRRLQKFQTWKRRQEELKQE G8YKJ9_PICSO/82-180 DELPDSVFEGFHRKMTKEEKSMSNVDKMKILSEVDNFRTCLETL.............KHNDWNRHIAEITHIENP...HDISELEEKRKLTIKELKFQLQRYEKWKRGYDSMSAD B5RUT9_DEBHA/85-183 DILDDSIYETFHKKMKKEERTMVNIDRSRILSEVDNLDLSLQSL.............NQYDWIRHLPNITQINDI...RDYDELETKKELTIKEIQRILNKFENWKRRQDKLVND A3LZL5_PICST/81-178 DPLPDKCYDVFHRKMKKEEKVMTNEDRLRILSEVDNLQTQLALL.............NQYDWIRHLPGIAFINDP...RDYEELEQKKLLTISEIERLIRKHENWRRRLDAITA. A5E7W5_LODEL/110-206 DPLPDSLYTIFHRKMRRDEKQMLNDELLRTLSELDTLQHYKQLL.............DQYDWIRHLPQITYIDNP...KDLQDMEWKKQLTLAEIDRLILRQVEWRKKRDEFV.. C5MDI7_CANTT/119-217 DPLPDSNYEIFHRKMRRNEKQMTNEEKIKNLWELDTLQSHLEDL.............NQLHWFKALPNITSIKDV...KDYQEMERKRDLTITEIERLLRKHDLWRRRQDIFNTD G8BH61_CANPC/85-183 DPLPDSKYTIFHRKMKKEETQMLNEEKIKNLVEVDNLNTSLQLL.............KQYDWIRHLPSITKVNDV...MDYEEMERKRELTINEIEILLEKQALWKRKRDKFNND C4Y5Z7_CLAL4/91-189 DPLPDQHFETFHRRMKREEKNMTGADRSKMMSEIETLKSQLRLL.............RQHDWMKHLPRITLVKDP...KDRSELQRKRELTEKECLRLLNKYYSWEKRNENLEND A5DMH2_PICGU/189-287 EPLPDDLYIGFHRRLKREETVMTNADISRLSSEIDRLKDHLRIL.............RSLDWARHLPTITVVNNR...SDSNEMNYKRKLTIGEIERLIANYDSWCRRQDRLRAE C5DKZ9_LACTC/98-193 DPLEDDLYLTFHKKMQKQETRMLNQDRLQSEGEAERLGVIYEEL.............GTNNWVKSLTKTTVIKNP...DDLHELELKRDLTREIIGQMLEKFKDMKTRAMLL... S6E1I9_ZYGB2/84-180 DALSDDLYLAYHKKMSRHEIRMLDSDLIQGENEADRLLLLSEKL.............DLAQWQTTLKKITVIRNP...DDENEMERKRLLTKETIDSMLKTYHTMKKNRTIAL.. C5DSY4_ZYGRC/86-184 DTLPDELYWAYHKKMTRHEARMLESDVIQGENEADKLSLWSEKL.............DLAHWQLKLKQITVIKNS...DDEEEMEKKRVLTKETIDSMLARYHTMKKNRSILLKN I2H7N4_TETBL/167-265 DLLADDKYKAFHKKMVRQEARMIEGDIIEAEQEAECLQLLRDKL.............LMPHWITTLLKATIVNDP...TDATELDNKRTLTMELLNHILERYNELLKLKTTLTKN G8ZXH3_TORDC/101-195 DLLSDALYNSFHKHMLRQETRMLEGDVAEGEAEAERLHLLSEKL.............DMIHWPITLRKVTVINDP...QDEDECSRKRELTKNCITSMLEKFEAMKNRGIM.... A7TLI0_VANPO/131-229 DPLSDALYKPYHKKMLKQENRMLERDVNEWETEADRLELLSDKL.............NMLDWHISLPKMTVINDV...CDEDEMDRKRELTKEYIRNTLAKYEEMKFQKSLLSKT G8BRL9_TETPH/141-238 DPLADEKYKPYHKKMLRQENRMIERDASETEREADRLLLLLDKL.............DMIDWKITLAKVTVINNI...EDAEEMNSKRVKTKELIQSMLAKYESTNLRASLFSK. H2ARA7_KAZAF/106-204 DPLPDKLYDSFHKKMLRQENRMLEQDVIEGEEEADGLQLIYDKL.............CMPNWPQTLSKVTKINDQ...ENMEELQLKKKLTRETIKSMLDKFHLMKKNSNMLYKN J7S3R9_KAZNA/141-239 DPLLDEVYSIYHKKMLKQENRMIEQDLVQEEDEADRLRLMYDKL.............NLPNWVSNLQKITKINNP...DDTVELDTKRRKTKIAIRGMLDKYDLTKNNSHVLHRS SAS4_YEAST/124-221 DLLPDSTYQPYHKKMLKQENRMIQSDIVNGENEADRLSLISDRL.............GMLNWEVTLQKVTKINDP...TDENEMETKRYQTKELIDSMLHKFESMKKKSRNLAR. Q6FU72_CANGA/98-195 .ALDDRKYINYHRRMLRQESRMIQLDITHGENEADRLLGVYDKL.............NMFNWQPTLLKVTKINDP...NDEEEMLAKREKTKEYIQTLLNNFDNMKKRTLSLNKK G0W381_NAUDC/162-257 DVLPDSLYTNFHKRMLRHENRMIDQDIIQSENEAERLTLINEKL.............DMIMWPTILQKVTKINDP...TDDDEMTRKRAQTKECIESMLEKFHSMKRRSNGL... G0VBR8_NAUCC/118-211 DPLPDSLYELFHKRMLRHENRMIEQDVIQGENEAERLTLIAEKL.............DMVTWPMTLQKVTKINDP...TDDDEMTRKRAQTIDSIKFMLEKFNLMKRKSI..... Q6CQC2_KLULA/86-184 DPLSDQLYDNYHKKMLKQETRMMNDESNHSDLEADRLEGILDHL.............EVASWRLYLPKVTIVKDL...FDEDELHAKKELTQRTIRLMLKKYKYMKHRINIHARK Q750S1_ASHGO/86-181 D...DDVYESYHRKMFKQETRMINDDKVESENGAERLQHISEAL.............ELPGWEQVLQQITVIGDA...SDAKELESKRKKTQIAIADMLTKFRDMKRRINVHSRN G8JTA8_ERECY/91-186 S...DEPYEAYHKKMFKQETRMINDDKIESENEAERLQHIYETL.............DMLGWEKVLAQVTVIRDV...DDQKELKTKRELTKLAIQDMLSKFQDMKRRISMQSRN K0KWZ0_WICCF/115-210 DPLIDELYHPYNKRMEKEEKKMINWEREKIYSEADKMKSQLEKL.............QQNNWSRILPTITYIRDP...RDQVELEQKKQWTIESLTLLLERFEHWKKLEDKV... K1XZH4_MARBU/283-379 DPLHDAYFESMHRRPERQEKTIRNTDKCRAQHEKDQVIRILESL.............QGHDWLKLMGVSG.VTE....SKKKEYEPARDYFIKGCETILEKFRIWREEEKRLKLE G2YXH1_BOTF4/264-360 DPLSEEHFKTLHKRPERQEKAVRNADKSRAQHERDSVIRLMEGL.............QGPDWLKTLGVSG.ITE....GRKKEFESARQYFIKGCESILEKFRIWKDDEKQRKLK S3CVW4_GLAL2/260-356 DPLSDEYFEKLHKKPERQEKAIRNSDKGRAQHEKDQIVRLLEGL.............QGHDWLKLMGVNG.VTD....SRRREYEPAREHFIKGCEAILEKFRNWKDEEKRRKLE L8G9Y1_PSED2/272-368 DPLPDSYYDIPHRRAKRLEMSIRNSEKGRAQHEKDHVARLLEGL.............QGHDWLKVMGVSG.ITE....SKKKEYEPAREHFIKGCQGILDKFRSWRDEEKRRKLE G0SGP7_CHATD/259-356 DPLPDSLFELPHKRAERQERSIRNTEKGRAKHEKEQVVRLLEGL.............QGPEWLRILGVHGPTTE....AKRRQYEPAREYFISGCQAILDKFKRWTAEEKRRKQM C7YJ20_NECH7/266-362 DPLPDKLFQPVHRRAERLERSIRNTEKGRAQHEKDQIIRLLEGL.............QGHDWLRVMGVSG.VTE....TKKKTFEPARDHFIKGCQAILAKFRNWVLEEKRRKQE U7PXI7_SPOS1/495-591 DPLPDSYFEAHHKRAERLERSIRNTEKGRAQHEKDQIMRLLEGL.............QGPDWLRTMGVSG.ITE....SKKKTFEPAREHFIRGSRAILEKFRLWSVEEKRRKLE G2Q6V6_MYCTT/295-391 DPLPDTLYSPAHRKAERLERSIRNTEKGRAQHEKDQIMRLLDGL.............QGHDWLRVMGVNG.ITE....SKKRAFEPARQHFIRGCENILSKFRRWAREEKRRRAE Q2HFJ0_CHAGB/261-357 DPLPDSLFAPAHKKAERSERSVRNTEIGRAQHERDQVARLLNGL.............RGHDWLRVLGVSG.VTE....TKKRAFEPARDHFIRGCEGILDKFRRYTREEKRRRAE G4N6E7_MAGO7/302-398 DPLPDALFEPVHKRAERAERSVRNTERGRAQHEKEQIIRLLDGL.............QGHDWLRVMGVSG.ITE....TKKKEFEEPRQHFIRGCEAILDKFRLWGVEEKRRKLE L2G7W8_COLGN/250-346 DPLPDASFVPSHKRAERLEKSIRNTERGRAQHEKDQIARLLGGL.............QGHDWLRVMGVSG.ITE....SRKKQYEPARQHFIKGCQAILERFRQWSLEEKRRKAE A7UVZ4_NEUCR/289-385 DPLPDSLYETAHKKAERLERSIRNSEKGRAQHEKDQIIRLLDAL.............QGHDWLRVMGVSG.ITE....GRKKQFEPAREHFIKGCQAILEKFRRWAAEEKRRKRK A0A0E1RY21_COCIM/119-215 DPLLNGTYFKAHRRIERKETQLRNIEKERAQHEKIQIDRLLSAL.............RGADWLRVMGVSG.IAE....SEKHLYEPKRTYFIMELAALLDKFKIWKQKEKRRKLK C4JIL8_UNCRE/78-174 DPLSNGVFFKAHRKNERKETQLRNIEKERAQHQQIQLDRLLVAL.............QGADWVRVMGISG.ITE....SEKKLYEPKRAYFIMELTALLEKFKFWKEKEKRRKAE G7XR06_ASPKW/336-433 DPLSEETYFRPHRRNERQEKQLRNIERDRAQHEKQNLDRLLDDL.............QGHDWLRVMGITGLISD....PERKHFESKRTYFIREISAVLQKFKIWREEEKRRKSE B8MXV1_ASPFN/339-436 DPLNEGTYFKAHRRNERQEKQLRNIERERAQHEKQQLDRLLDEL.............QNHDWLRVMGITGLLSD....QEKKQYEPKRDYFIKEISALIQKFKIWKEEEKRRKVE Q0D1Q9_ASPTN/340-434 DPLSEATYFRAHRRNERQEKQLRNIERDRAQHEKQQLDRLLDEL.............QSQDWPRVMGIGT.LTDH...EEKKQYETKRAFFVKEIAASALEIQNL...ERRRKAT Q4WCB1_ASPFU/365-461 DPLNDGYYFRAHRRMERQEKQLRNIERERAQHEKMQLERLLDEL.............KGHDWLRVMGITG.VHE....HEKKLYEPKRDYFMKEIASLIEKFQIWKEEEKRRKLD B6HFN3_PENRW/301-397 DPLSEETYFRAHRKFERQEKQLRNIERNRAQHEQQVLERLLDEL.............RGHDWLRMMGLTG.VHE....SDKKLYEPKRDILIQELVTLVNKFQAWKDEERRRKLE S7ZMG8_PENO1/348-444 DPLTEDLYFRAHRRFERQEKHLRNIERDRAQHEKQHLDRLLEEL.............RSQDWLRVMGLTG.VHE....NEKKLYEPKRKILIDELVVLVNKFQKWKDEERKRKLA #=GC seq_cons DPLsDshYtthH++hcRpE+phpNt-+tRuppEt-pLpplL-pL.............pstDWl+slshss.Is-....s-ccchE.KRphhIcplpslLcKFcpWKccccchchc //