Summary: Centromere kinetochore component CENP-T histone fold
Centromere kinetochore component CENP-T histone fold Provide feedback
CENP-T is a family of vertebral kinetochore proteins that associates directly with CENP-W. The N-terminus of CENP-T proteins interacts directly with the Ndc80 complex in the outer kinetochore. Importantly, the CENP-T-W complex does not directly associate with CENP-A, but with histone H3 in the centromere region. CENP-T and -W form a hetero-tetramer with CENP-S and -X and bind to a ~100 bp region of nucleosome-free DNA forming a nucleosome-like structure. The DNA-CENP-T-W-S-X complex is likely to be associated with histone H3-containing nucleosomes rather than with CENP-nucleosomes. This domain is the C-terminal histone fold domain of CENP-T, which associates with chromatin [2-3].
Suzuki A, Hori T, Nishino T, Usukura J, Miyagi A, Morikawa K, Fukagawa T;, J Cell Biol. 2011;193:125-140.: Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins. PUBMED:21464230 EPMC:21464230
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T;, Cell. 2012;148:487-501.: CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold. PUBMED:22304917 EPMC:22304917
Internal database links
|Similarity to PfamA using HHSearch:||Histone CBFD_NFYB_HMF TFIID-31kDa CENP-S|
External database links
This tab holds annotation information from the InterPro database.
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Members of this clan all possess a histone fold. Generally proteins in this clan are DNA binding.
The clan contains the following 13 members:Bromo_TP Bromo_TP_like CBFD_NFYB_HMF CENP-S CENP-T_C CENP-W CENP-X Histone TAF TAFII28 TFIID-18kDa TFIID-31kDa TFIID_20kDa
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Curation and family details
|Seed source:||Pfam-B_9162 (release 26.0)|
|Author:||Coggill P, Eberhardt R|
|Number in seed:||11|
|Number in full:||1814|
|Average length of the domain:||82.40 aa|
|Average identity of full alignment:||64 %|
|Average coverage of the sequence by the domain:||49.58 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||2|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CENP-T_C domain has been found. There are 264 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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