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3  structures 243  species 0  interactions 476  sequences 9  architectures

Family: SLC3A2_N (PF16028)

Summary: Solute carrier family 3 member 2 N-terminus

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This is the Wikipedia entry entitled "SLC3A2". More...

SLC3A2 Edit Wikipedia article

SLC3A2
Protein SLC3A2 PDB 2dh2.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, solute carrier family 3 member 2
External IDs MGI: 96955 HomoloGene: 1795 GeneCards: SLC3A2
Gene location (Human)
Chromosome 11 (human)
Chr. Chromosome 11 (human)[1]
Chromosome 11 (human)
Genomic location for SLC3A2
Genomic location for SLC3A2
Band 11q12.3 Start 62,856,102 bp[1]
End 62,888,875 bp[1]
RNA expression pattern
PBB GE SLC3A2 200924 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001161413
NM_008577

RefSeq (protein)

NP_001012680
NP_001012682
NP_001013269
NP_002385

NP_001154885
NP_032603

Location (UCSC) Chr 11: 62.86 – 62.89 Mb Chr 19: 8.71 – 8.72 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

4F2 cell-surface antigen heavy chain is a protein that in humans is encoded by the SLC3A2 (solute carrier family 3 member 2) gene.[5][6]

SLC3A2 comprises the heavy subunit of the large neutral amino acid transporter (LAT1) that is also known as CD98 (cluster of differentiation 98).[7][8]

Function

SLC3A2 is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha-amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.[6]

LAT1 is a heterodimeric membrane transport protein that preferentially transports neutral branched (valine, leucine, isoleucine) and aromatic (tryptophan, tyrosine) amino acids.[9] LAT is highly expressed in brain capillaries (which form the blood brain barrier) relative to other tissues.[9]

A functional LAT1 transporter is composed of two proteins encoded by two distinct genes:

  • 4F2hc/CD98 heavy subunit protein encoded by the SLC3A2 gene (this gene)[10]
  • CD98 light subunit protein encoded by the SLC7A5 gene[11]

Interactions

SLC3A2 has been shown to interact with SLC7A7.[12]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000168003 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000010095 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ Teixeira S, Di Grandi S, Kühn LC (Aug 1987). "Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus". J Biol Chem. 262 (20): 9574–80. PMID 3036867. 
  6. ^ a b "Entrez Gene: SLC3A2 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2". 
  7. ^ Kucharzik T, Lugering A, Yan Y, Driss A, Charrier L, Sitaraman S, Merlin D (2005). "Activation of epithelial CD98 glycoprotein perpetuates colonic inflammation". Lab. Invest. 85 (7): 932–41. doi:10.1038/labinvest.3700289. PMID 15880135. 
  8. ^ Lemaître G, Gonnet F, Vaigot P, Gidrol X, Martin MT, Tortajada J, Waksman G (2005). "CD98, a novel marker of transient amplifying human keratinocytes". Proteomics. 5 (14): 3637–45. doi:10.1002/pmic.200401224. PMID 16097038. 
  9. ^ a b Boado RJ, Li JY, Nagaya M, Zhang C, Pardridge WM (1999). "Selective expression of the large neutral amino acid transporter at the blood–brain barrier". Proc. Natl. Acad. Sci. U.S.A. 96 (21): 12079–84. doi:10.1073/pnas.96.21.12079. PMC 18415Freely accessible. PMID 10518579. 
  10. ^ Palacín M, Kanai Y (2004). "The ancillary proteins of HATs: SLC3 family of amino acid transporters". Pflügers Arch. 447 (5): 490–494. doi:10.1007/s00424-003-1062-7. PMID 14770309. 
  11. ^ Verrey F, Closs EI, Wagner CA, Palacin M, Endou H, Kanai Y (2004). "CATs and HATs: the SLC7 family of amino acid transporters". Pflügers Arch. 447 (5): 532–542. doi:10.1007/s00424-003-1086-z. PMID 14770310. 
  12. ^ Pfeiffer R, Rossier G, Spindler B, Meier C, Kühn L, Verrey F (Jan 1999). "Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family". EMBO J. 18 (1): 49–57. doi:10.1093/emboj/18.1.49. PMC 1171101Freely accessible. PMID 9878049. 

Further reading

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Solute carrier family 3 member 2 N-terminus Provide feedback

This domain is found at the N-terminus of solute carrier family 3 member 2 proteins (4F2 cell-surface antigen heavy chain).

Literature references

  1. Pfeiffer R, Rossier G, Spindler B, Meier C, Kuhn L, Verrey F;, EMBO J. 1999;18:49-57.: Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family. PUBMED:9878049 EPMC:9878049


This tab holds annotation information from the InterPro database.

InterPro entry IPR031984

Heteromeric amino acid transporters (HATs) are composed of two subunits, a heavy (SLC3 family) and a light subunit [SLC7 or L-type amino acid transporter (LAT) family] linked by a conserved disulfide bridge [PUBMED:23506863]. HATs are amino acid exchangers, and the transport activity resides in the light subunit. The heavy chain of the surface antigen 4F2 (4F2hc), also known as solute carrier family 3 member 2 protein (Slc3a2), is a HAT heavy subunit [PUBMED:9915839, PUBMED:24516142]. 4F2hc controls the cell surface expression as well as the membrane topology of the 4F2 heterodimer [PUBMED:9915839].

This entry represents the N-terminal domain of 4F2hc.

Domain organisation

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Alignments

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  Seed
(26)
Full
(476)
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(770)
NCBI
(946)
Meta
(0)
RP15
(146)
RP35
(246)
RP55
(368)
RP75
(428)
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Key: ✓ available, x not generated, not available.

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  Seed
(26)
Full
(476)
Representative proteomes UniProt
(770)
NCBI
(946)
Meta
(0)
RP15
(146)
RP35
(246)
RP55
(368)
RP75
(428)
Alignment:
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  Seed
(26)
Full
(476)
Representative proteomes UniProt
(770)
NCBI
(946)
Meta
(0)
RP15
(146)
RP35
(246)
RP55
(368)
RP75
(428)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Jackhmmer:Q9VDG3
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Eberhardt R
Number in seed: 26
Number in full: 476
Average length of the domain: 75.50 aa
Average identity of full alignment: 34 %
Average coverage of the sequence by the domain: 15.40 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.8
Noise cut-off 26.6 26.8
Model length: 77
Family (HMM) version: 5
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the SLC3A2_N domain has been found. There are 3 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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