Summary: Glycosyl hydrolase family 99
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Glycosyl hydrolase family 99 Provide feedback
This domain, around 350 residues, is mainly found in some uncharacterized proteins from bacteroides to human. Some proteins in this family, annotated as endo-alpha-mannosidases cleave mannoside linkages internally within an N-linked glycan chain, short circuiting the classical N-glycan biosynthetic pathway. This domain reveals a (beta-alpha)(8) barrel fold in which the catalytic centre is present in a long substrate-binding groove, consistent with cleavage within the N-glycan chain, providing a foundation upon which to develop new enzyme inhibitors targeting the hijacking of N-glycan synthesis in viral disease and cancer .
Thompson AJ, Williams RJ, Hakki Z, Alonzi DS, Wennekes T, Gloster TM, Songsrirote K, Thomas-Oates JE, Wrodnigg TM, Spreitz J, StÃ¼tz AE, Butters TD, Williams SJ, Davies GJ;, Proc. Natl. Acad. Sci. U.S.A. 109 (3), 781-786 (2012).: Structural and mechanistic insight into N-glycan processing by endo-Î±-mannosidase. PUBMED:22219371 EPMC:22219371
Internal database links
|Similarity to PfamA using HHSearch:||Glyco_hydro_71 Glyco_tran_WbsX DUF4434|
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR026071
This family contains endomannosidases and endomannosidase-like proteins. Glycoprotein endo-alpha-1,2-mannosidase hydrolyses the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of the glycoprotein produced in the Golgi membrane [ PUBMED:15760709 , PUBMED:15677381 ].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||hydrolase activity, acting on glycosyl bonds (GO:0016798)|
- the number of sequences which exhibit this architecture
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This example describes an architecture with one
Gladomain, followed by two consecutive
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- the UniProt description of the protein sequence
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This large superfamily contains a range of glycosyl hydrolase enzymes that possess a TIM barrel fold. This CLAN merges clans GH-A, GH-D, GH-H and GH-K from CAZy.
The clan contains the following 59 members:Alpha-amylase Alpha_L_fucos Cellulase Cellulase-like Dehydratase_LU DUF1906 DUF4015 DUF4038 DUF4434 DUF4838 DUF4849 DUF5010 DUF5696 DUF6259 GHL10 GHL13 GHL15 GHL5 GHL6 Glyco_hydr_30_2 Glyco_hydro_1 Glyco_hydro_10 Glyco_hydro_101 Glyco_hydro_114 Glyco_hydro_129 Glyco_hydro_14 Glyco_hydro_17 Glyco_hydro_18 Glyco_hydro_20 Glyco_hydro_25 Glyco_hydro_26 Glyco_hydro_2_C Glyco_hydro_3 Glyco_hydro_30 Glyco_hydro_31 Glyco_hydro_35 Glyco_hydro_39 Glyco_hydro_42 Glyco_hydro_44 Glyco_hydro_53 Glyco_hydro_56 Glyco_hydro_59 Glyco_hydro_66 Glyco_hydro_70 Glyco_hydro_71 Glyco_hydro_72 Glyco_hydro_77 Glyco_hydro_79n Glyco_hydro_85 Glyco_hydro_97 Glyco_hydro_99 Glyco_hydro_cc Glyco_tran_WbsX hDGE_amylase Melibiase Melibiase_2 NAGidase NAGLU Raffinose_syn
We make a range of alignments for each Pfam-A family:
- the curated alignment from which the HMM for the family is built
- the alignment generated by searching the sequence database using the HMM
- Representative Proteomes (RPs) at 15%, 35%, 55% and 75% co-membership thresholds
- alignment generated by searching the UniProtKB sequence database using the family HMM
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Curation and family details
|Seed source:||Jackhmmer BT_3863 (NP_812773.1) and PDB 4ACY|
|Author:||Chang Y , Eberhardt R|
|Number in seed:||4|
|Number in full:||887|
|Average length of the domain:||286.00 aa|
|Average identity of full alignment:||43 %|
|Average coverage of the sequence by the domain:||68.29 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_99 domain has been found. There are 53 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
|Protein||Predicted structure||External Information|
|E7EYY9||View 3D Structure||Click here|
|F1M1G2||View 3D Structure||Click here|
|Q1L8D2||View 3D Structure||Click here|
|Q5GF25||View 3D Structure||Click here|
|Q5SRI9||View 3D Structure||Click here|
|Q5VSG8||View 3D Structure||Click here|
|Q6NXH2||View 3D Structure||Click here|
|Q6P1J0||View 3D Structure||Click here|
|Q9VMQ2||View 3D Structure||Click here|