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35  structures 1244  species 0  interactions 4963  sequences 215  architectures

Family: NtCtMGAM_N (PF16863)

Summary: N-terminal barrel of NtMGAM and CtMGAM, maltase-glucoamylase

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

N-terminal barrel of NtMGAM and CtMGAM, maltase-glucoamylase Provide feedback

NtCtMGAM_N is a beta-barrel-like structure just N-terminal to the catalytic domain of maltase-glucoamylase in eukaryotes. It contributes to the architecture of the substrate-binding site, by donating a loop that comes into close contact with two regions in the catalytic domain thereby creating the site [1]. This family is frequently found at the N-terminus of Glycosyl hydrolase 31, PF01055.to which it contributes as above.

Literature references

  1. Sim L, Quezada-Calvillo R, Sterchi EE, Nichols BL, Rose DR;, J Mol Biol. 2008;375:782-792.: Human intestinal maltase-glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity. PUBMED:18036614 EPMC:18036614


This tab holds annotation information from the InterPro database.

InterPro entry IPR031727

This is a beta-barrel-like structure just N-terminal to the catalytic domain of maltase-glucoamylase in eukaryotes. It contributes to the architecture of the substrate-binding site by donating a loop that comes into close contact with two regions in the catalytic domain, thereby creating the site [ PUBMED:18036614 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Gal_mutarotase (CL0103), which has the following description:

This clan is composed of a beta-sandwich that was first observed in domain 5 of beta-galactosidase, then as the central domain of copper amine oxidase, the C-terminal domain of chondroitinase, the C-terminal domain of hyaluronate lyase, the N-terminal domain of maltose phosphorylase and in Galactose Mutarotase [1]. All these enzymes act on a sugar substrate.

The clan contains the following 32 members:

AGL_N Aldose_epim AmyA-gluTrfs_C Bgal_small_N Cu_amine_oxid DUF4432 DUF4450 DUF5054 DUF5107 DUF5127 DUF5703_N Gal_mutarotas_2 Gal_mutarotas_3 GH97_N Glucodextran_N Glyco_hyd_65N_2 Glyco_hydr_116N Glyco_hydro_36N Glyco_hydro_38 Glyco_hydro_38C Glyco_hydro_52 Glyco_hydro_65N Glyco_hydro_81 Glyco_hydro_92N Glyco_transf_36 Hepar_II_III Lyase_8 MdoG NtCtMGAM_N Rhamnogal_lyase RhgB_N YidC_periplas

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(309)
Full
(4963)
Representative proteomes UniProt
(7717)
RP15
(820)
RP35
(2016)
RP55
(3818)
RP75
(5094)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(309)
Full
(4963)
Representative proteomes UniProt
(7717)
RP15
(820)
RP35
(2016)
RP55
(3818)
RP75
(5094)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(309)
Full
(4963)
Representative proteomes UniProt
(7717)
RP15
(820)
RP35
(2016)
RP55
(3818)
RP75
(5094)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: pdb_2qly
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Coggill P
Number in seed: 309
Number in full: 4963
Average length of the domain: 114.00 aa
Average identity of full alignment: 32 %
Average coverage of the sequence by the domain: 12.72 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.8 26.8
Trusted cut-off 26.8 26.8
Noise cut-off 26.7 26.7
Model length: 114
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the NtCtMGAM_N domain has been found. There are 35 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2KY72 View 3D Structure Click here
A0A0G2L1N0 View 3D Structure Click here
A0A0R0I6Y6 View 3D Structure Click here
A0A1D6LPQ2 View 3D Structure Click here
A0A1D6MM31 View 3D Structure Click here
A0A2R8QK09 View 3D Structure Click here
A0A571BDS5 View 3D Structure Click here
A1CNK4 View 3D Structure Click here
A1D1E6 View 3D Structure Click here
B8MZ41 View 3D Structure Click here
D3ZTX4 View 3D Structure Click here
D4B0X3 View 3D Structure Click here
E7FCP7 View 3D Structure Click here
F4J6T7 View 3D Structure Click here
F8VQM5 View 3D Structure Click here
I1J6K4 View 3D Structure Click here
I1KWV4 View 3D Structure Click here
I1LEX2 View 3D Structure Click here
I1MG74 View 3D Structure Click here
O43451 View 3D Structure Click here
O74254 View 3D Structure Click here
P07768 View 3D Structure Click here
P10253 View 3D Structure Click here
P14410 View 3D Structure Click here
P23739 View 3D Structure Click here
P70699 View 3D Structure Click here
Q09901 View 3D Structure Click here
Q0CMA7 View 3D Structure Click here
Q0JQZ2 View 3D Structure Click here
Q12558 View 3D Structure Click here
Q19004 View 3D Structure Click here
Q21750 View 3D Structure Click here
Q2M2H8 View 3D Structure Click here
Q2UQV7 View 3D Structure Click here
Q4WRH9 View 3D Structure Click here
Q55D50 View 3D Structure Click here
Q5AWI5 View 3D Structure Click here
Q5R7A9 View 3D Structure Click here
Q653V4 View 3D Structure Click here
Q653V7 View 3D Structure Click here