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11  structures 1259  species 0  interactions 1682  sequences 9  architectures

Family: 4PPT_N (PF17837)

Summary: 4'-phosphopantetheinyl transferase N-terminal domain

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4'-phosphopantetheinyl transferase N-terminal domain Provide feedback

This entry represents the N-terminal domain from 4'- phosphopantetheinyl transferase enzymes. This domain is structurally related to the PF01648 domain with which it forms a pseudodimeric arrangement [2].

Literature references

  1. Parris KD, Lin L, Tam A, Mathew R, Hixon J, Stahl M, Fritz CC, Seehra J, Somers WS;, Structure. 2000;8:883-895.: Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites. PUBMED:10997907 EPMC:10997907

  2. Vickery CR, Kosa NM, Casavant EP, Duan S, Noel JP, Burkart MD;, ACS Chem Biol. 2014;9:1939-1944.: Structure, biochemistry, and inhibition of essential 4'-phosphopantetheinyl transferases from two species of Mycobacteria. PUBMED:24963544 EPMC:24963544

  3. Halavaty AS, Kim Y, Minasov G, Shuvalova L, Dubrovska I, Winsor J, Zhou M, Onopriyenko O, Skarina T, Papazisi L, Kwon K, Peterson SN, Joachimiak A, Savchenko A, Anderson WF;, Acta Crystallogr D Biol Crystallogr. 2012;68:1359-1370.: Structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria. PUBMED:22993090 EPMC:22993090

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR041354

This entry represents the N-terminal domain from 4'- phosphopantetheinyl transferase enzymes. This domain is structurally related to the 4'-phosphopantetheinyl transferase domain ( INTERPRO ) with which it forms a pseudodimeric arrangement [ PUBMED:24963544 ].

This domain can also be found in enterobactin synthase component D (EntD), which forms part of the enterobactin-synthetase enzyme complex. It plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively [ PUBMED:9214294 ]. Deletion studies involving EntD- mutants have shown that it is essential for virulence [ PUBMED:2521622 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan 4PPT (CL0670), which has the following description:

This superfamily includes the two related domains found in 4'-phosphopantetheinyl transferase enzymes.

The clan contains the following 2 members:



We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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Representative proteomes UniProt

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: ECOD:EUF02159
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 33
Number in full: 1682
Average length of the domain: 67.80 aa
Average identity of full alignment: 38 %
Average coverage of the sequence by the domain: 28.51 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.0
Noise cut-off 26.9 26.9
Model length: 68
Family (HMM) version: 4
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the 4PPT_N domain has been found. There are 11 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
O33336 View 3D Structure Click here
P0A3B9 View 3D Structure Click here
P0A3C0 View 3D Structure Click here
P19925 View 3D Structure Click here
Q56064 View 3D Structure Click here