Summary: 6-pyruvoyl tetrahydropterin synthase
6-pyruvoyl tetrahydropterin synthase Provide feedback
6-Pyruvoyl tetrahydrobiopterin synthase catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. The functional enzyme is a hexamer of identical subunits .
Nar H, Huber R, Heizmann CW, Thony B, Burgisser D; , EMBO J 1994;13:1255-1262.: Three-dimensional structure of 6-pyruvoyl tetrahydropterin synthase, an enzyme involved in tetrahydrobiopterin biosynthesis. PUBMED:8137809 EPMC:8137809
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR007115
6-Pyruvoyl tetrahydrobiopterin synthase (PTPS) catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP [PUBMED:8137809]. The functional enzyme is a hexamer of identical subunits. A transition metal binding site formed by the three histidine residues 23, 48 and 50 is present in each subunit, and bound Zn(II) is responsible for the enzymatic activity. Site-directed mutagenesis of each of these three histidine residues results in a complete loss of metal binding and enzymatic activity [PUBMED:7563095, PUBMED:9165069].
The function of the bacterial branch of the family had been thought to be the same, but many are now taken to be QueD, and enzyme of queuosine biosynthesis. Queuosine is a hypermodified base in the wobble position of some tRNAs in most species [PUBMED:14660578].
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Curation and family details
|Author:||Finn RD, Bateman A|
|Number in seed:||149|
|Number in full:||3812|
|Average length of the domain:||126.40 aa|
|Average identity of full alignment:||30 %|
|Average coverage of the sequence by the domain:||87.70 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||14|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the PTPS domain has been found. There are 68 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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