Summary: RWD domain
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This domain was identified in WD40 repeat proteins and Ring finger domain proteins . The function of this domain is unknown. GCN2 is the alpha-subunit of the only translation initiation factor (eIF2 alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain  after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices .
Doerks T, Copley RR, Schultz J, Ponting CP, Bork P; , Genome Res 2002;12:47-56.: Systematic identification of novel protein domain families associated with nuclear functions. PUBMED:11779830 EPMC:11779830
Nameki N, Yoneyama M, Koshiba S, Tochio N, Inoue M, Seki E, Matsuda T, Tomo Y, Harada T, Saito K, Kobayashi N, Yabuki T, Aoki M, Nunokawa E, Matsuda N, Sakagami N, Terada T, Shirouzu M, Yoshida M, Hirota H, Osanai T, Tanaka A, Arakawa T, Carninci P, Kawai, Protein Sci 2004;13:2089-2100.: Solution structure of the RWD domain of the mouse GCN2 protein. PUBMED:15273307 EPMC:15273307
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR006575
The RWD eukaryotic domain is found in RING finger (INTERPRO) and WD repeat (INTERPRO) containing proteins and DEXDc-like helicase (INTERPRO) subfamily related to the ubiquitin-conjugating enzymes domain (INTERPRO).
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||protein binding (GO:0005515)|
- the number of sequences which exhibit this architecture
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This example describes an architecture with one
Gladomain, followed by two consecutive
EGFdomains, and finally a single
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We make a range of alignments for each Pfam-A family:
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Curation and family details
|Seed source:||Pfam-B_3991 (release 8.0)|
|Number in seed:||119|
|Number in full:||1878|
|Average length of the domain:||119.30 aa|
|Average identity of full alignment:||20 %|
|Average coverage of the sequence by the domain:||19.67 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null --hand HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||17|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the RWD domain has been found. There are 8 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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