Summary: Oligosaccharyl transferase STT3 subunit
Oligosaccharyl transferase STT3 subunit Provide feedback
This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity . In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER . In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines [3-4].
Hong G, Deleersnijder W, Kozak CA, Van Marck E, Tylzanowski P, Merregaert J; , Genomics 1996;31:295-300.: Molecular cloning of a highly conserved mouse and human integral membrane protein (Itm1) and genetic mapping to mouse chromosome 9. PUBMED:8838310 EPMC:8838310
Zufferey R, Knauer R, Burda P, Stagljar I, te Heesen S, Lehle L, Aebi M; , EMBO J 1995;14:4949-4960.: STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo. PUBMED:7588624 EPMC:7588624
Abu-Qarn M, Yurist-Doutsch S, Giordano A, Trauner A, Morris HR, Hitchen P, Medalia O, Dell A, Eichler J;, J Mol Biol. 2007;374:1224-1236.: Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. PUBMED:17996897 EPMC:17996897
Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D;, EMBO J. 2008;27:234-243.: Structure-guided identification of a new catalytic motif of oligosaccharyltransferase. PUBMED:18046457 EPMC:18046457
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR003674
N-linked glycosylation is a ubiquitous protein modification, and is essential for viability in eukaryotic cells. A lipid-linked core-oligosaccharide is assembled at the membrane of the endoplasmic reticulum and transferred to selected asparagine residues of nascent polypeptide chains by the oligosaccharyl transferase (OTase) complex [PUBMED:7588624].
This family consists of the oligsacharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosccharyl transferase (OTase) complex of proteins and is required for its activity [PUBMED:7588624].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||membrane (GO:0016020)|
|Molecular function||oligosaccharyl transferase activity (GO:0004576)|
|Biological process||protein glycosylation (GO:0006486)|
- the number of sequences which exhibit this architecture
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This example describes an architecture with one
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This is the GT-C clan that contains diverse glycosyltransferases that possess 8-13 predicted transmembrane segments .
The clan contains the following 21 members:ALG3 Alg6_Alg8 Arabinose_trans DIE2_ALG10 DUF1420 DUF2029 DUF2079 DUF2142 DUF2723 EpsG Glucan_synthase Glyco_transf_22 Mannosyl_trans Mannosyl_trans2 Oleosin PIG-U PMT PMT_2 PTPS_related STT3 YfhO
We make a range of alignments for each Pfam-A family:
- the curated alignment from which the HMM for the family is built
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- Representative Proteomes (RPs) at 15%, 35%, 55% and 75% co-membership thresholds
- alignment generated by searching the NCBI sequence database using the family HMM
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Curation and family details
|Seed source:||Pfam-B_1095 (release 5.4)|
|Author:||Bashton M, Bateman A|
|Number in seed:||15|
|Number in full:||968|
|Average length of the domain:||459.70 aa|
|Average identity of full alignment:||26 %|
|Average coverage of the sequence by the domain:||63.95 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||9|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the STT3 domain has been found. There are 11 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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