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0  structures 411  species 0  interactions 1178  sequences 15  architectures

Family: Steroid_dh (PF02544)

Summary: 3-oxo-5-alpha-steroid 4-dehydrogenase

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3-oxo-5-alpha-steroid 4-dehydrogenase Provide feedback

This family consists of 3-oxo-5-alpha-steroid 4-dehydrogenases, EC:1.3.99.5 Also known as Steroid 5-alpha-reductase, the reaction catalysed by this enzyme is: 3-oxo-5-alpha-steroid + acceptor <=> 3-oxo-delta(4)-steroid + reduced acceptor. The Steroid 5-alpha-reductase enzyme is responsible for the formation of dihydrotestosterone, this hormone promotes the differentiation of male external genitalia and the prostate during fetal development [2]. In humans mutations in this enzyme can cause a form of male pseudohermaphorditism in which the external genitalia and prostate fail to develop normally [2]. A related enzyme is also found in plants is Q38944 (DET2) a steroid reductase from Arabidopsis. Mutations in this enzyme cause defects in light-regulated development [1].

Literature references

  1. Li J, Nagpal P, Vitart V, McMorris TC, Chory J; , Science 1996;272:398-401.: A role for brassinosteroids in light-dependent development of Arabidopsis PUBMED:8602526 EPMC:8602526

  2. Jenkins EP, Hsieh CL, Milatovich A, Normington K, Berman DM, Francke U, Russell DW; , Genomics 1991;11:1102-1112.: Characterization and chromosomal mapping of a human steroid 5 alpha-reductase gene and pseudogene and mapping of the mouse homologue. PUBMED:1686016 EPMC:1686016

  3. Andersson S, Berman DM, Jenkins EP, Russell DW; , Nature 1991;354:159-161.: Deletion of steroid 5 alpha-reductase 2 gene in male pseudohermaphroditism. PUBMED:1944596 EPMC:1944596


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001104

Synonym(s): Steroid 5-alpha-reductase

3-oxo-5-alpha-steroid 4-dehydrogenases, EC catalyse the conversion of 3-oxo-5-alpha-steroid + acceptor to 3-oxo-delta(4)-steroid + reduced acceptor. The steroid 5-alpha-reductase enzyme is responsible for the formation of dihydrotestosterone, this hormone promotes the differentiation of male external genitalia and the prostate during foetal development [PUBMED:1686016]. In humans mutations in this enzyme can cause a form of male pseudohermaphorditism in which the external genitalia and prostate fail to develop normally. A related steroid reductase enzyme, DET2, is found in plants such as Arabidopsis. Mutations in this enzyme cause defects in light-regulated development [PUBMED:8602526]. This domain is present in both type 1 and type 2 forms.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Steroid_dh (CL0115), which has the following description:

This clan includes several enzymes, including steroid dehydrogenases and isoprenylcysteine carboxyl methyltransferase enzymes. These protein contain a varying number of transmembrane regions.

The clan contains the following 6 members:

DUF1295 ERG4_ERG24 ICMT NnrU PEMT Steroid_dh

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(10)
Full
(1178)
Representative proteomes NCBI
(1677)
Meta
(1096)
RP15
(231)
RP35
(405)
RP55
(592)
RP75
(741)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(10)
Full
(1178)
Representative proteomes NCBI
(1677)
Meta
(1096)
RP15
(231)
RP35
(405)
RP55
(592)
RP75
(741)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(10)
Full
(1178)
Representative proteomes NCBI
(1677)
Meta
(1096)
RP15
(231)
RP35
(405)
RP55
(592)
RP75
(741)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1713 (release 5.4)
Previous IDs: none
Type: Family
Author: Bashton M, Bateman A
Number in seed: 10
Number in full: 1178
Average length of the domain: 142.80 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 50.78 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.8 20.8
Trusted cut-off 20.8 20.8
Noise cut-off 20.7 20.7
Model length: 150
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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