Summary: hTAFII28-like protein conserved region
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hTAFII28-like protein conserved region Provide feedback
The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. The conserved region is found at the C-terminal of most member proteins. The crystal structure of hTAFII28 with hTAFII18 shows that this region is involved in the binding of these two subunits. The conserved region contains four alpha helices and three loops arranged as in histone H3 [1,2].
Mengus G, May M, Jacq X, Staub A, Tora L, Chambon P, Davidson I; , EMBO J 1995;14:1520-1531.: Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID. PUBMED:7729427 EPMC:7729427
Birck C, Poch O, Romier C, Ruff M, Mengus G, Lavigne AC, Davidson I, Moras D; , Cell 1998;94:239-249.: Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family. PUBMED:9695952 EPMC:9695952
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR006809The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. The conserved region is found at the C terminus of most member proteins. The crystal structure of hTAFII28 with hTAFII18 shows that this region is involved in the binding of these two subunits. The conserved region contains four alpha helices and three loops arranged as in histone H3 [PUBMED:7729427, PUBMED:9695952].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||nucleus (GO:0005634)|
|Biological process||transcription initiation from RNA polymerase II promoter (GO:0006367)|
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Members of this clan all possess a histone fold. Generally proteins in this clan are DNA binding.
The clan contains the following 11 members:Bromo_TP CBFD_NFYB_HMF CENP-S CENP-T CENP-W Histone TAF TAFII28 TFIID-18kDa TFIID-31kDa TFIID_20kDa
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Curation and family details
|Seed source:||Pfam-B_4085 (release 7.5)|
|Author:||Waterfield DI, Finn RD|
|Number in seed:||10|
|Number in full:||332|
|Average length of the domain:||87.60 aa|
|Average identity of full alignment:||40 %|
|Average coverage of the sequence by the domain:||33.70 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||9|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TAFII28 domain has been found. There are 2 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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