Summary: Toprim domain
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Toprim domain Provide feedback
The toprim domain is found in a wide variety of enzymes involved in nucleic acid manipulation .
Aravind L, Leipe DD, Koonin EV; , Nucleic Acids Res 1998;26:4205-4213.: Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins. PUBMED:9722641 EPMC:9722641
Internal database links
|SCOOP:||Toprim Toprim_2 Toprim_4|
|Similarity to PfamA using HHSearch:||Toprim DUF3854 Toprim_2 Toprim_4|
This tab holds annotation information from the InterPro database.
InterPro entry IPR006171
The Toprim (topoisomerase-primase) domain is a structurally conserved domain of ~100 amino acids that is found in bacterial DnaG-type primases, small primase-like proteins from bacteria and archaea, type IA and type II topoisomerases, bacterial and archaeal nucleases of the OLD family and bacterial DNA repair proteins of the RecR/M family. The Toprim domain can be found alone or in combination with several other domains, such as the ASM domain, the superfamily 2 helicase domain, the superfamily 3 helicase domain, the DnaB interaction domain, the C4 'little finger' domain, the CHC2 zinc finger, the ATPase domain of the HSP90-gyrase-histidine kinase superfamily, the S5 domain, the SET domain, the helix-hairpin-helix (HhH) DNA-binding domain, the mobilisation (MOB) domain or the ATPase domain of the ABC transporter/SMC superfamily. The Toprim domain is a catalytic domain involved in DNA strand breakage and rejoining [PUBMED:9722641].
The Toprim domain has two conserved motifs, one of which centres at a conserved glutamate and the other one at two conserved aspartates (DxD). Both motifs are preceded by conserved hydrophobic regions predicted to form beta-strands. The glutamate residue is probably involved in catalysis, whereas the DxD motif is involved in the co-ordination of Mg(2++) that is required for the activity of all Toprim-containing enzymes. The Toprim domain has a compact alpha/beta fold, with four conserved strands and three helices; with the exception of the second helix and the C-terminal strands, each of these elements contains positions that are highly conserved. The Toprim domain contains three regions that can accommodate variable sized inserts, which are particularly prominent in the topoisomerases [PUBMED:9722641, PUBMED:16077031, PUBMED:19596812].
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This is families that have a conserved region from DNA primase. This corresponds to the Toprim domain common to DnaG primases, topoisomerases, OLD family nucleases and RecR proteins .
The clan contains the following 7 members:DUF2220 DUF2399 DUF3854 Toprim Toprim_2 Toprim_3 Toprim_4
We make a range of alignments for each Pfam-A family:
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- alignment generated by searching the UniProtKB sequence database using the family HMM
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- alignment generated by searching the metagenomics sequence database using the family HMM
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Curation and family details
|Number in seed:||22|
|Number in full:||1050|
|Average length of the domain:||95.70 aa|
|Average identity of full alignment:||26 %|
|Average coverage of the sequence by the domain:||20.32 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||5|
|Download:||download the raw HMM for this family|
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