Summary: Bacterial conjugation TrbI-like protein
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Bacterial conjugation TrbI-like protein Provide feedback
Although not essential for conjugation, the TrbI protein greatly increase the conjugational efficiency .
Alt-Morbe J, Stryker JL, Fuqua C, Li PL, Farrand SK, Winans SC; , J Bacteriol 1996;178:4248-4257.: The conjugal transfer system of Agrobacterium tumefaciens octopine-type Ti plasmids is closely related to the transfer system of an IncP plasmid and distantly related to Ti plasmid vir genes. PUBMED:8763954 EPMC:8763954
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This tab holds annotation information from the InterPro database.
InterPro entry IPR005498
This entry represents type IV secretion system proteins VirB10, TraB and TrbI. Type IV secretion systems are found in plant and animal pathogens, as well as in symbiotic bacteria.
The tumour-inducing (Ti) plasmid of Rhizobium radiobacter (Agrobacterium tumefaciens) encodes various DNA transfer systems: VirB, Tra and Trb, where the virB operon is required for the transfer DNA to the plant host and the tra/trb systems are required for the conjugal transfer of the Ti plasmid between cells of Agrobacterium [PUBMED:9679196, PUBMED:12169609]. Of the two unlinked regions of octopine-type Ti plasmids that contain genes required for conjugal transfer, the tra gene cluster is probably required for conjugal DNA processing, whilst the other gene cluster, trb, probably directs the synthesis of a conjugal pilus and mating pore [PUBMED:8763954]. It has been suggested that TraB extends into the periplasmic space and is anchored in the inner membrane via a single transmembrane segment near the N terminus. It is also thought that TraB may interact with TraP, in order to stabilise the proposed transmembrane complex formed by the tra operon products [PUBMED:8655498].
The trbI gene encodes a 128-amino-acid polypeptide that is an intrinsic inner-membrane protein. TrbI may influence the kinetics of pilus outgrowth and/or retraction [PUBMED:1355084]. Although not essential for conjugation, the TrbI protein greatly increases conjugational efficiency [PUBMED:8763954]. In Plasmid pTiC58, all of the trb genes except trbI and trbK are essential for conjugal transfer [PUBMED:10438776].
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Curation and family details
|Seed source:||Pfam-B_776 (release 7.0)|
|Number in seed:||149|
|Number in full:||1918|
|Average length of the domain:||183.10 aa|
|Average identity of full alignment:||23 %|
|Average coverage of the sequence by the domain:||39.41 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||9|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TrbI domain has been found. There are 20 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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