Summary: YqaJ-like viral recombinase domain
This is the Wikipedia entry entitled "YqaJ protein domain". More...
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YqaJ protein domain Edit Wikipedia article
|YqaJ protein domain|
Toroidal structure of lambda exonuclease, determined at 2.4 angstroms
In molecular biology, the YqaJ refers to the YqaJ/K domain from the skin prophage of the bacterium, Bacillus subtilis. This protein domain, often found in bacterial species, is actually of viral origin. The protein forms an oligomer and functions as an alkaline exonuclease, or in simpler terms, an enzyme that digests double-stranded DNA. It is a reaction which is dependent on Magnesium. It has a preference for 5'-phosphorylated DNA ends. It thus forms part of the two-component SynExo viral recombinase functional unit.
The function of this protein domain is to digest DNA. Most viruses, inject their host with linear DNA, and this gets incorporated into the host genome through the process of recombination. This recombination is crucial to viral replication.
YqaJ is a one of three protein subunits that form a toroid with a tapered channel passing through the middle. The channel changes diameter, the wide end of the channel being about 30 Å, and the narrow end decreases to 15 Å. It is thought that the tapered channel is large enough to accommodate double-stranded DNA at the wide end but only single-stranded DNA at the other end. Furthermore, YqaJ has an alpha/beta fold.
SynExo is a viral recombinase functional unit. It is thought that it may have evolved as a portable module that can function wide variety of host organisms without requiring extensive interaction with host-specific functions. This offers the pathogen a great adaptive advantage on viruses exploring new niches.
- Vellani TS, Myers RS (April 2003). "Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases". J. Bacteriol. 185 (8): 2465–74. doi:10.1128/jb.185.8.2465-2474.2003. PMC 152610. PMID 12670970.
- Kovall R, Matthews BW (1997). "Toroidal structure of lambda-exonuclease.". Science 277 (5333): 1824–7. doi:10.1126/science.277.5333.1824. PMID 9295273.
YqaJ-like viral recombinase domain Provide feedback
This protein family is found in many different bacterial species but is of viral origin. The protein forms an oligomer and functions as a processive alkaline exonuclease that digests linear double-stranded DNA in a Mg(2+)-dependent reaction, It has a preference for 5'-phosphorylated DNA ends. It thus forms part of the two-component SynExo viral recombinase functional unit .
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR019080
This protein is found in many different bacterial species but is of viral origin. The protein forms an oligomer and functions as a processive alkaline exonuclease that digests linear double-stranded DNA in a Mg(2+)-dependent reaction, It has a preference for 5'-phosphorylated DNA ends. It thus forms part of the two-component SynExo viral recombinase functional unit [PUBMED:12670970].
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This clan includes a large number of nuclease families related to holliday junction resolvases [1,2].
The clan contains the following 123 members:BamHI BpuJI_N BpuSI_N Bse634I BsuBI_PstI_RE Cas_APE2256 Cas_Cas02710 Cas_Cas4 Cas_Csm6 Cas_NE0113 CoiA Dna2 DpnI DpnII DUF1016 DUF1052 DUF1780 DUF1887 DUF2034 DUF2161 DUF234 DUF2357 DUF2726 DUF2800 DUF2887 DUF3799 DUF3883 DUF4143 DUF4263 DUF4420 DUF559 DUF91 DUF911 EcoRI EcoRII-C eIF-3_zeta Endonuc-BglII Endonuc-BsobI Endonuc-EcoRV Endonuc-FokI_C Endonuc-HincII Endonuc-MspI Endonuc-PvuII Endonuc_BglI Endonuc_Holl ERCC4 Exo5 Flu_PA Herpes_alk_exo Herpes_UL24 Hjc HSDR_N HSDR_N_2 L_protein_N McrBC Mrr_cat Mrr_cat_2 MutH MvaI_BcnI NaeI NERD NgoMIV_restric NotI PDDEXK_1 PDDEXK_10 PDDEXK_2 PDDEXK_3 PDDEXK_4 PDDEXK_5 PDDEXK_7 PDDEXK_9 Pet127 Phage_endo_I R-HINP1I Rad10 RAI1 RAP RE_AlwI RE_ApaLI RE_Bpu10I RE_Bsp6I RE_CfrBI RE_Eco47II RE_EcoO109I RE_HaeII RE_HindIII RE_HindVP RE_HpaII RE_LlaJI RE_LlaMI RE_MjaI RE_NgoBV RE_NgoPII RE_SacI RE_ScaI RE_SinI RE_TaqI RE_TdeIII RE_XamI RE_XcyI RecU RestrictionMunI RestrictionSfiI RmuC RNA_pol_Rpb5_N Sen15 SfsA Spo0A_C TBPIP_N ThaI Tn7_Tnp_TnsA_N Transposase_31 tRNA_int_endo Tsp45I Uma2 UPF0102 VirArc_Nuclease VRR_NUC Vsr XhoI XisH YaeQ YqaJ
We make a range of alignments for each Pfam-A family:
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Curation and family details
|Seed source:||Pfam-B_3587 (release 21.0)|
|Number in seed:||26|
|Number in full:||5594|
|Average length of the domain:||138.90 aa|
|Average identity of full alignment:||33 %|
|Average coverage of the sequence by the domain:||51.15 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||6|
|Download:||download the raw HMM for this family|
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There is 1 interaction for this family. More...
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the YqaJ domain has been found. There are 15 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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