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0  structures 343  species 0  interactions 474  sequences 18  architectures

Family: Zw10 (PF06248)

Summary: Centromere/kinetochore Zw10

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Centromere/kinetochore Zw10 Provide feedback

Zw10 and rough deal proteins are both required for correct metaphase check-pointing during mitosis [1,2]. These proteins bind to the centromere/kinetochore [2].

Literature references

  1. Basto R, Gomes R, Karess RE; , Nat Cell Biol 2000;2:939-943.: Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila. PUBMED:11146659 EPMC:11146659

  2. Chan GK, Jablonski SA, Starr DA, Goldberg ML, Yen TJ; , Nat Cell Biol 2000;2:944-947.: Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. PUBMED:11146660 EPMC:11146660


This tab holds annotation information from the InterPro database.

InterPro entry IPR009361

Zeste white 10 (ZW10) was initially identified as a mitotic checkpoint protein involved in chromosome segregation, and then implicated in targeting cytoplasmic dynein and dynactin to mitotic kinetochores, but it is also important in non-dividing cells. These include cytoplasmic dynein targeting to Golgi and other membranes, and SNARE-mediated ER-Golgi trafficking [PUBMED:17102640, PUBMED:16505164]. Dominant-negative ZW10, anti-ZW10 antibody, and ZW10 RNA interference (RNAi) cause Golgi dispersal. ZW10 RNAi also disperse endosomes and lysosomes [PUBMED:16505164].

Drosophila kinetochore components Rough deal (Rod) and Zw10 are required for the proper functioning of the metaphase checkpoint in flies [PUBMED:11146659]. The eukaryotic spindle assembly checkpoint (SAC) monitors microtubule attachment to kinetochores and prevents anaphase onset until all kinetochores are aligned on the metaphase plate. It is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. In higher eukaryotes, cytoplasmic dynein is involved in silencing the SAC by removing the checkpoint proteins Mad2 and the Rod-Zw10-Zwilch complex (RZZ) from aligned kinetochores [PUBMED:17576797, PUBMED:18268100, PUBMED:17509882].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Vps51 (CL0295), which has the following description:

This clan includes an N-terminal domain from several vesicle transport proteins that are related to Vps51.

The clan contains the following 17 members:

COG2 COG5 COG6 Dor1 Exo70 Exo84_C Sec15 Sec3_C Sec3_C_2 Sec5 Sec6 Sec8_exocyst Vps51 Vps52 Vps53_N Vps54_N Zw10

Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(8)
Full
(474)
Representative proteomes UniProt
(837)
NCBI
(1003)
Meta
(0)
RP15
(109)
RP35
(239)
RP55
(350)
RP75
(438)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

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  Seed
(8)
Full
(474)
Representative proteomes UniProt
(837)
NCBI
(1003)
Meta
(0)
RP15
(109)
RP35
(239)
RP55
(350)
RP75
(438)
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(8)
Full
(474)
Representative proteomes UniProt
(837)
NCBI
(1003)
Meta
(0)
RP15
(109)
RP35
(239)
RP55
(350)
RP75
(438)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_9476 (release 9.0)
Previous IDs: none
Type: Family
Author: Finn RD
Number in seed: 8
Number in full: 474
Average length of the domain: 417.10 aa
Average identity of full alignment: 17 %
Average coverage of the sequence by the domain: 59.29 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 17690987 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 29.8 29.8
Trusted cut-off 29.8 29.8
Noise cut-off 29.6 29.7
Model length: 595
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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