Summary: cwf21 domain
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The cwf21 family is involved in mRNA splicing. It has been isolated as a subcomplex of the splicosome in Schizosaccharomyces pombe . The function of the cwf21 domain is to bind directly to the spliceosomal protein Prp8. Mutations in the cwf21 domain prevent Prp8 from binding . The structure of this domain has recently been solved which shows this domain to be composed of two alpha helices.
Ohi MD, Link AJ, Ren L, Jennings JL, McDonald WH, Gould KL; , Mol Cell Biol 2002;22:2011-2024.: Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. PUBMED:11884590 EPMC:11884590
Grainger RJ, Barrass JD, Jacquier A, Rain JC, Beggs JD;, RNA. 2009; [Epub ahead of print]: Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome. PUBMED:19854871 EPMC:19854871
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR013170
The cwf21 domain is found in proteins involved in mRNA splicing. Proteins containing this domain have been isolated as a subcomplex of the splicosome in Schizosaccharomyces pombe (Fission yeast) [PUBMED:11884590]. In yeast, this domain binds the protein Prp8p [PUBMED:19854871], a large and highly conserved U5 snRNP protein which has been proposed as a protein cofactor at the spliceosomal catalytic centre [PUBMED:11017191].
The cwf21 domain is found in, amongst others, the small Cwc21p protein in yeast as well as in the much larger human ortholog SRm300 (serine/arginine repetitive matrix protein).
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Curation and family details
|Seed source:||Pfam-B_14400 (release 17.0)|
|Author:||Mistry J, Wood V, Bateman A|
|Number in seed:||76|
|Number in full:||461|
|Average length of the domain:||48.80 aa|
|Average identity of full alignment:||38 %|
|Average coverage of the sequence by the domain:||7.17 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the cwf21 domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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