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199  structures 8775  species 0  interactions 59159  sequences 392  architectures

Family: GATase (PF00117)

Summary: Glutamine amidotransferase class-I

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This is the Wikipedia entry entitled "Glutamine amidotransferase". More...

Glutamine amidotransferase Edit Wikipedia article

Glutamine amidotransferase class-I
PDB 1o1y EBI.jpg
crystal structure of putative glutamine amido transferase (tm1158) from thermotoga maritima at 1.70 a resolution
Identifiers
SymbolGATase
PfamPF00117
Pfam clanCL0014
InterProIPR000991
PROSITEPDOC00406
MEROPSC44
SCOPe1ea0 / SUPFAM
CDDcd01653

In molecular biology, glutamine amidotransferases (GATase) are enzymes which catalyse the removal of the ammonia group from a glutamine molecule and its subsequent transfer to a specific substrate, thus creating a new carbon-nitrogen group on the substrate. This activity is found in a range of biosynthetic enzymes, including glutamine amidotransferase, anthranilate synthase component II, p-aminobenzoate, and glutamine-dependent carbamoyl-transferase (CPSase). Glutamine amidotransferase (GATase) domains can occur either as single polypeptides, as in glutamine amidotransferases, or as domains in a much larger multifunctional synthase protein, such as CPSase. On the basis of sequence similarities two classes of GATase domains have been identified: class-I (also known as trpG-type) and class-II (also known as purF-type).[1][2] Class-I GATase domains are defined by a conserved catalytic triad consisting of cysteine, histidine and glutamate. Class-I GATase domains have been found in the following enzymes: the second component of anthranilate synthase and 4-amino-4-deoxychorismate (ADC) synthase; CTP synthase; GMP synthase; glutamine-dependent carbamoyl-phosphate synthase; phosphoribosylformylglycinamidine synthase II; and the histidine amidotransferase hisH.

References

  1. ^ Weng ML, Zalkin H (July 1987). "Structural role for a conserved region in the CTP synthetase glutamine amide transfer domain". Journal of Bacteriology. 169 (7): 3023–8. PMC 212343. PMID 3298209.
  2. ^ Nyunoya H, Lusty CJ (August 1984). "Sequence of the small subunit of yeast carbamyl phosphate synthetase and identification of its catalytic domain". The Journal of Biological Chemistry. 259 (15): 9790–8. PMID 6086650.
This article incorporates text from the public domain Pfam and InterPro: IPR000991

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Glutamine amidotransferase class-I Provide feedback

No Pfam abstract.

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR017926

Glutamine amidotransferase (GATase) enzymes catalyse the removal of the ammonia group from glutamine and then transfer this group to a substrate to form a new carbon-nitrogen group [ PUBMED:4355768 ]. The GATase domain exists either as a separate polypeptidic subunit or as part of a larger polypeptide fused in different ways to a synthase domain. Two classes of GATase domains have been identified [ PUBMED:3298209 , PUBMED:6086650 ]: class-I (also known as trpG-type or triad) and class-II (also known as purF-type or Ntn). Class-I (or type 1) GATase domains have been found in the following enzymes:

  • The second component of anthranilate synthase (AS) [ PUBMED:2679363 ]. AS catalyzes the biosynthesis of anthranilate from chorismate and glutamine. AS is generally a dimeric enzyme: the first component can synthesize anthranilate using ammonia rather than glutamine, whereas component II provides the GATase activity [ PUBMED:10449718 ]. In some bacteria and in fungi the GATase component of AS is part of a multifunctional protein that also catalyzes other steps of the biosynthesis of tryptophan.
  • The second component of 4-amino-4-deoxychorismate (ADC) synthase, a dimeric prokaryotic enzyme that functions in the pathway that catalyzes the biosynthesis of para-aminobenzoate (PABA) from chorismate and glutamine. The second component (gene pabA) provides the GATase activity [ PUBMED:2679363 ].
  • CTP synthase. CTP synthase catalyzes the final reaction in the biosynthesis of pyrimidine, the ATP-dependent formation of CTP from UTP and glutamine. CTP synthase is a single chain enzyme that contains two distinct domains; the GATase domain is in the C-terminal section [ PUBMED:3298209 ].
  • GMP synthase (glutamine-hydrolyzing). GMP synthase catalyzes the ATP-dependent formation of GMP from xanthosine 5'-phosphate and glutamine. GMP synthase is a single chain enzyme that contains two distinct domains; the GATase domain is in the N-terminal section [ PUBMED:2982857 , PUBMED:8548458 ].
  • Glutamine-dependent carbamoyl-phosphate synthase (GD-CPSase); an enzyme involved in both arginine and pyrimidine biosynthesis and which catalyzes the ATP-dependent formation of carbamoyl phosphate from glutamine and carbon dioxide. In bacteria GD-CPSase is composed of two subunits: the large chain (gene carB) provides the CPSase activity, while the small chain (gene carA) provides the GATase activity. In yeast the enzyme involved in arginine biosynthesis is also composed of two subunits: CPA1 (GATase), and CPA2 (CPSase). In most eukaryotes, the first three steps of pyrimidine biosynthesis are catalyzed by a large multifunctional enzyme (called URA2 in yeast, rudimentary in Drosophila, and CAD in mammals). The GATase domain is located at the N-terminal extremity of this polyprotein [ PUBMED:8098212 ].
  • Phosphoribosylformylglycinamidine synthase, an enzyme that catalyzes the fourth step in the de novo biosynthesis of purines. In some species of bacteria and rchaea, FGAM synthase II is composed of two subunits: a small chain (gene purQ) which provides the GATase activity and a large chain (gene purL) which provides the aminator activity. In eukaryotes and Gram-negative bacteria a single polypeptide (large type of purL) contains a FGAM synthethase domain and the GATase as the C-terminal domain [ PUBMED:15301531 ].
  • Imidazole glycerol phosphate synthase subunit hisH, an enzyme that catalyzes the fifth step in the biosynthesis of histidine.
A triad of conserved Cys-His-Glu forms the active site, wherein the catalytic cysteine is essential for the amidotransferase activity [ PUBMED:8548458 , PUBMED:9575335 ]. Different structures show that the active site Cys of type 1 GATase is located at the tip of a nucleophile elbow.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Glutaminase_I (CL0014), which has the following description:

Most members of this clan are glutaminase enzymes. This superfamily is shown to be related in [1]. The clan also contains the DJ-1/PfpI family that includes the peptidase PfpI that has a catalytic Cys-His-Glu triad that differs from the class I GAT Cys-His-Glu triad.

The clan contains the following 18 members:

ABC_transp_aux BPL_N Catalase_C DJ-1_PfpI DUF4159 GATase GATase1_like GATase_3 GATase_5 Glyco_hydro_42M HTS LBP_M Peptidase_C26 Peptidase_S51 Peptidase_S66 SNO ThiJ_like ThuA

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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  Seed
(96)
Full
(59159)
Representative proteomes UniProt
(257592)
RP15
(8046)
RP35
(27802)
RP55
(57181)
RP75
(95565)
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  Seed
(96)
Full
(59159)
Representative proteomes UniProt
(257592)
RP15
(8046)
RP35
(27802)
RP55
(57181)
RP75
(95565)
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  Seed
(96)
Full
(59159)
Representative proteomes UniProt
(257592)
RP15
(8046)
RP35
(27802)
RP55
(57181)
RP75
(95565)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Seed source: Prosite
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Sonnhammer ELL
Number in seed: 96
Number in full: 59159
Average length of the domain: 191.00 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 43.11 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.8 22.8
Trusted cut-off 22.8 22.8
Noise cut-off 22.7 22.7
Model length: 191
Family (HMM) version: 30
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the GATase domain has been found. There are 199 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0V5B4 View 3D Structure Click here
A0A0P0WQH1 View 3D Structure Click here
A0A0P0WQQ3 View 3D Structure Click here
A0A0R0EDM9 View 3D Structure Click here
A0A0R0EDM9 View 3D Structure Click here
A0A0R0EQ27 View 3D Structure Click here
A0A0R0EQ27 View 3D Structure Click here
A0A1D6DT59 View 3D Structure Click here
A0A1D6E921 View 3D Structure Click here
A0A1D6EQP4 View 3D Structure Click here
A0A1D6FZE0 View 3D Structure Click here
A0A1D6G0N7 View 3D Structure Click here
A0A1D6GKB5 View 3D Structure Click here
A0A1D6H537 View 3D Structure Click here
A0A1D6I9R6 View 3D Structure Click here
A0A1D6IXB4 View 3D Structure Click here
A0A1D6J566 View 3D Structure Click here
A0A1D6KZL1 View 3D Structure Click here
A0A1D6L291 View 3D Structure Click here
A0A1D6L7F2 View 3D Structure Click here
A0A1D6LNP0 View 3D Structure Click here
A0A1D6LNP0 View 3D Structure Click here
A0A1D6LNP0 View 3D Structure Click here
A0A1D6LW74 View 3D Structure Click here
A0A1D6MDH1 View 3D Structure Click here
A0A1D6MXC5 View 3D Structure Click here
A0A1D6NH72 View 3D Structure Click here
A0A1D6NMD0 View 3D Structure Click here
A0A1D6P6W2 View 3D Structure Click here
A0A1D8PFC8 View 3D Structure Click here
A0A1D8PMB0 View 3D Structure Click here
A0A1D8PRE9 View 3D Structure Click here
A0A1D8PTD1 View 3D Structure Click here
A0A1P8B6F9 View 3D Structure Click here
A0A2R8Q0M3 View 3D Structure Click here
A0A2R8QCI7 View 3D Structure Click here
A4HWV8 View 3D Structure Click here
A4HYQ5 View 3D Structure Click here
B1WC02 View 3D Structure Click here
B2RQC6 View 3D Structure Click here
B4FQM8 View 3D Structure Click here
B8JLW8 View 3D Structure Click here
C0PH60 View 3D Structure Click here
C6TIQ8 View 3D Structure Click here
D4A8A0 View 3D Structure Click here
E9AGZ1 View 3D Structure Click here
F4I6G9 View 3D Structure Click here
F4INN2 View 3D Structure Click here
G5EC98 View 3D Structure Click here
G5EC98 View 3D Structure Click here
I1JBI0 View 3D Structure Click here
I1JWW2 View 3D Structure Click here
I1K4V8 View 3D Structure Click here
I1K6H8 View 3D Structure Click here
I1KBF7 View 3D Structure Click here
I1KL62 View 3D Structure Click here
I1KPB0 View 3D Structure Click here
I1KQX3 View 3D Structure Click here
I1KTT7 View 3D Structure Click here
I1KW26 View 3D Structure Click here
I1KWW6 View 3D Structure Click here
I1L7R7 View 3D Structure Click here
I1M2L9 View 3D Structure Click here
I1MIA7 View 3D Structure Click here
I1MJH0 View 3D Structure Click here
I1MMQ9 View 3D Structure Click here
I1MSY9 View 3D Structure Click here
I1N0Y1 View 3D Structure Click here
K7L2D7 View 3D Structure Click here
K7LA93 View 3D Structure Click here
K7LKM5 View 3D Structure Click here
K7LKM5 View 3D Structure Click here
K7MFE5 View 3D Structure Click here
K7MG22 View 3D Structure Click here
K7MQT5 View 3D Structure Click here
K7TZS8 View 3D Structure Click here
K7URM3 View 3D Structure Click here
K7VDM7 View 3D Structure Click here
O42644 View 3D Structure Click here
O60060 View 3D Structure Click here
O64753 View 3D Structure Click here
O82225 View 3D Structure Click here
O94277 View 3D Structure Click here
O94303 View 3D Structure Click here
P00903 View 3D Structure Click here
P00904 View 3D Structure Click here
P00937 View 3D Structure Click here
P04079 View 3D Structure Click here
P05990 View 3D Structure Click here
P07258 View 3D Structure Click here
P07259 View 3D Structure Click here
P07756 View 3D Structure Click here
P0A6F1 View 3D Structure Click here
P0A7E5 View 3D Structure Click here
P17812 View 3D Structure Click here
P20054 View 3D Structure Click here
P27708 View 3D Structure Click here
P28274 View 3D Structure Click here
P31327 View 3D Structure Click here
P32073 View 3D Structure Click here
P33734 View 3D Structure Click here
P37254 View 3D Structure Click here
P38625 View 3D Structure Click here
P38627 View 3D Structure Click here
P49915 View 3D Structure Click here
P60595 View 3D Structure Click here
P70303 View 3D Structure Click here
P70698 View 3D Structure Click here
P9WHK7 View 3D Structure Click here
P9WMM1 View 3D Structure Click here
P9WMS7 View 3D Structure Click here
P9WN35 View 3D Structure Click here
P9WPK5 View 3D Structure Click here
Q09580 View 3D Structure Click here
Q09686 View 3D Structure Click here
Q09794 View 3D Structure Click here
Q0WRY1 View 3D Structure Click here
Q10F14 View 3D Structure Click here
Q10NW0 View 3D Structure Click here
Q12288 View 3D Structure Click here
Q18990 View 3D Structure Click here
Q2FUU1 View 3D Structure Click here
Q2FWD1 View 3D Structure Click here
Q2FYR8 View 3D Structure Click here
Q2FZ73 View 3D Structure Click here
Q2G099 View 3D Structure Click here
Q2G0Y6 View 3D Structure Click here
Q2QNR7 View 3D Structure Click here
Q3THK7 View 3D Structure Click here
Q42565 View 3D Structure Click here
Q4CP83 View 3D Structure Click here
Q4D6X8 View 3D Structure Click here
Q4DI98 View 3D Structure Click here
Q4DQH7 View 3D Structure Click here
Q4E1D4 View 3D Structure Click here
Q4V7C6 View 3D Structure Click here
Q54DH6 View 3D Structure Click here
Q54V77 View 3D Structure Click here
Q54XU8 View 3D Structure Click here
Q553V7 View 3D Structure Click here
Q57690 View 3D Structure Click here
Q57929 View 3D Structure Click here
Q58425 View 3D Structure Click here
Q58574 View 3D Structure Click here
Q58970 View 3D Structure Click here
Q59T45 View 3D Structure Click here
Q5AK79 View 3D Structure Click here
Q5AML6 View 3D Structure Click here
Q5APF2 View 3D Structure Click here
Q5U2N0 View 3D Structure Click here
Q5VPD3 View 3D Structure Click here
Q5Z856 View 3D Structure Click here
Q5Z856 View 3D Structure Click here
Q65XK2 View 3D Structure Click here
Q69QJ1 View 3D Structure Click here
Q6ETL5 View 3D Structure Click here
Q6PEI7 View 3D Structure Click here
Q6YV23 View 3D Structure Click here
Q764B9 View 3D Structure Click here
Q7XUS2 View 3D Structure Click here
Q8C196 View 3D Structure Click here
Q8H4X9 View 3D Structure Click here
Q8I2T0 View 3D Structure Click here
Q8IEN3 View 3D Structure Click here
Q8IJR9 View 3D Structure Click here
Q8ILZ3 View 3D Structure Click here
Q8ILZ3 View 3D Structure Click here
Q8LPN3 View 3D Structure Click here
Q8LPN3 View 3D Structure Click here
Q92370 View 3D Structure Click here
Q9CAD1 View 3D Structure Click here
Q9FE37 View 3D Structure Click here
Q9FJM5 View 3D Structure Click here
Q9FXK1 View 3D Structure Click here
Q9LTW8 View 3D Structure Click here
Q9LVW7 View 3D Structure Click here
Q9M0A5 View 3D Structure Click here
Q9M0A6 View 3D Structure Click here
Q9M0A7 View 3D Structure Click here
Q9NRF8 View 3D Structure Click here
Q9P772 View 3D Structure Click here
Q9SZ30 View 3D Structure Click here
Q9VIE7 View 3D Structure Click here
Q9VUL1 View 3D Structure Click here
U3JAV8 View 3D Structure Click here