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1040  structures 260  species 0  interactions 8429  sequences 247  architectures

Family: Lectin_legB (PF00139)

Summary: Legume lectin domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Leguminous lectin family". More...

Leguminous lectin family Edit Wikipedia article

Lectin_legB
PDB 1rin EBI.jpg
x-ray crystal structure of a pea lectin-trimannoside complex at 2.6 angstroms resolution
Identifiers
Symbol Lectin_legB
Pfam PF00139
Pfam clan CL0004
InterPro IPR001220
PROSITE PDOC00278
SCOP 1lem
SUPERFAMILY 1lem

In molecular biology, the leguminous lectin family is a family of lectin proteins.

It is one of the largest lectin families with more than 70 lectins reported in a review in 1990.[1] Leguminous lectins consist of two or four subunits, each containing one carbohydrate-binding site. The interaction with sugars requires tightly bound calcium and manganese ions. The structural similarities of these lectins are reported by the primary structural analyses and X-ray crystallographic studies.[2][3] X-ray studies have shown that the folding of the polypeptide chains in the region of the carbohydrate-binding sites is also similar, despite differences in the primary sequences. The carbohydrate-binding sites of these lectins consist of two conserved amino acids on beta pleated sheets. One of these loops contains transition metals, calcium and manganese, which keep the amino acid residues of the sugar-binding site at the required positions. Amino acid sequences of this loop play an important role in the carbohydrate-binding specificities of these lectins. These lectins bind either glucose, mannose or galactose. The exact function of legume lectins is not known but they may be involved in the attachment of nitrogen-fixing bacteria to legumes and in the protection against pathogens.[4][5]

Some legume lectins are proteolytically processed to produce two chains, beta (which corresponds to the N-terminal) and alpha (C-terminal). The lectin concanavalin A (conA) from jack bean is exceptional in that the two chains are transposed and ligated (by formation of a new peptide bond). The N terminus of mature conA thus corresponds to that of the alpha chain and the C terminus to the beta chain.[6]

References

  1. ^ Sharon N, Lis H (1990). "Legume lectins--a large family of homologous proteins.". FASEB J. 4 (14): 3198–208. PMID 2227211. 
  2. ^ de Oliveira TM, Delatorre P, da Rocha BA, de Souza EP, Nascimento KS, Bezerra GA, et al. (2008). "Crystal structure of Dioclea rostrata lectin: insights into understanding the pH-dependent dimer-tetramer equilibrium and the structural basis for carbohydrate recognition in Diocleinae lectins.". J Struct Biol. 164 (2): 177–82. PMID 18682294. doi:10.1016/j.jsb.2008.05.012. 
  3. ^ Rozwarski DA, Swami BM, Brewer CF, Sacchettini JC (1998). "Crystal structure of the lectin from Dioclea grandiflora complexed with core trimannoside of asparagine-linked carbohydrates.". J Biol Chem. 273 (49): 32818–25. PMID 9830028. doi:10.1074/jbc.273.49.32818. 
  4. ^ Roopashree S, Singh SA, Gowda LR, Rao AG (2006). "Dual-function protein in plant defence: seed lectin from Dolichos biflorus (horse gram) exhibits lipoxygenase activity.". Biochem J. 395 (3): 629–39. PMC 1462680Freely accessible. PMID 16441240. doi:10.1042/BJ20051889. 
  5. ^ Beringer JE, Brewin N, Johnston AW, Schulman HM, Hopwood DA (1979). "The Rhizobium--legume symbiosis.". Proc R Soc Lond B Biol Sci. 204 (1155): 219–33. PMID 36624. doi:10.1098/rspb.1979.0024. 
  6. ^ Carrington DM, Auffret A, Hanke DE (1985). "Polypeptide ligation occurs during post-translational modification of concanavalin A.". Nature. 313 (5997): 64–7. PMID 3965973. doi:10.1038/313064a0. 

This article incorporates text from the public domain Pfam and InterPro IPR001220

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Legume lectin domain Provide feedback

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Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001220

Lectins are carbohydrate-binding proteins. Leguminous lectins form one of the largest lectin families and resemble each other in their physicochemical properties, though they differ in their carbohydrate specificities. They bind either glucose/mannose or galactose [ PUBMED:2227211 ]. Carbohydrate-binding activity depends on the simultaneous presence of both a calcium and a transition metal ion [ PUBMED:9546043 ]. The exact function of legume lectins is not known, but they may be involved in the attachment of nitrogen-fixing bacteria to legumes and in the protection against pathogens [ PUBMED:16441240 , PUBMED:36624 ].

Some legume lectins are proteolytically processed to produce two chains, beta (which corresponds to the N-terminal) and alpha (C-terminal) [ PUBMED:8373823 ]. The lectin concanavalin A (conA) from jack bean is exceptional in that the two chains are transposed and ligated (by formation of a new peptide bond). The N terminus of mature conA thus corresponds to that of the alpha chain and the C terminus to the beta chain [ PUBMED:3965973 ]. Though the legume lectins monomer is structurally well conserved, their quaternary structures vary widely [ PUBMED:9546043 ].

Gene Ontology

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Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(78)
Full
(8429)
Representative proteomes UniProt
(12728)
RP15
(728)
RP35
(4385)
RP55
(7003)
RP75
(9246)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(78)
Full
(8429)
Representative proteomes UniProt
(12728)
RP15
(728)
RP35
(4385)
RP55
(7003)
RP75
(9246)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(78)
Full
(8429)
Representative proteomes UniProt
(12728)
RP15
(728)
RP35
(4385)
RP55
(7003)
RP75
(9246)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: lectin_legB;
Type: Domain
Sequence Ontology: SO:0000417
Author: Sonnhammer ELL , Bateman A
Number in seed: 78
Number in full: 8429
Average length of the domain: 220.10 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 39.01 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.3 26.3
Trusted cut-off 26.3 26.3
Noise cut-off 26.2 26.2
Model length: 249
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Lectin_legB domain has been found. There are 1040 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0N7KP75 View 3D Structure Click here
A0A0N7KP76 View 3D Structure Click here
A0A0N7KQF2 View 3D Structure Click here
A0A0N7KS46 View 3D Structure Click here
A0A0P0VG87 View 3D Structure Click here
A0A0P0VHY6 View 3D Structure Click here
A0A0P0VIP0 View 3D Structure Click here
A0A0P0W3M6 View 3D Structure Click here
A0A0P0W456 View 3D Structure Click here
A0A0P0W6A9 View 3D Structure Click here
A0A0P0W6B9 View 3D Structure Click here
A0A0P0W6G5 View 3D Structure Click here
A0A0P0W6Q6 View 3D Structure Click here
A0A0P0W6Z5 View 3D Structure Click here
A0A0P0W716 View 3D Structure Click here
A0A0P0W774 View 3D Structure Click here
A0A0P0X232 View 3D Structure Click here
A0A0P0X248 View 3D Structure Click here
A0A0P0X2C3 View 3D Structure Click here
A0A0P0X2L9 View 3D Structure Click here
A0A0P0X5B1 View 3D Structure Click here
A0A0P0X7N1 View 3D Structure Click here
A0A0P0XB61 View 3D Structure Click here
A0A0P0XB84 View 3D Structure Click here
A0A0P0XBC7 View 3D Structure Click here
A0A0P0XEE0 View 3D Structure Click here
A0A0P0XEL8 View 3D Structure Click here
A0A0P0XI97 View 3D Structure Click here
A0A0P0XK25 View 3D Structure Click here
A0A0P0XLY8 View 3D Structure Click here
A0A0R0EBT5 View 3D Structure Click here
A0A0R0EC29 View 3D Structure Click here
A0A0R0EC29 View 3D Structure Click here
A0A0R0EVP4 View 3D Structure Click here
A0A0R0EXH6 View 3D Structure Click here
A0A0R0F078 View 3D Structure Click here
A0A0R0FGZ5 View 3D Structure Click here
A0A0R0FH08 View 3D Structure Click here
A0A0R0FPN3 View 3D Structure Click here
A0A0R0G6N2 View 3D Structure Click here
A0A0R0G7S7 View 3D Structure Click here
A0A0R0GBL0 View 3D Structure Click here
A0A0R0GGF4 View 3D Structure Click here
A0A0R0H7W3 View 3D Structure Click here
A0A0R0HY71 View 3D Structure Click here
A0A0R0HYY4 View 3D Structure Click here
A0A0R0HYY4 View 3D Structure Click here
A0A0R0I316 View 3D Structure Click here
A0A0R0IHZ9 View 3D Structure Click here
A0A0R0IHZ9 View 3D Structure Click here
A0A0R0II11 View 3D Structure Click here
A0A0R0IPF3 View 3D Structure Click here
A0A0R0IT89 View 3D Structure Click here
A0A0R0JMR6 View 3D Structure Click here
A0A0R0JQV4 View 3D Structure Click here
A0A0R0KN88 View 3D Structure Click here
A0A0R0KS97 View 3D Structure Click here
A0A0R0L0T5 View 3D Structure Click here
A0A0R0LHG8 View 3D Structure Click here
A0A1D6E1S5 View 3D Structure Click here
A0A1D6EDA5 View 3D Structure Click here
A0A1D6EDB8 View 3D Structure Click here
A0A1D6EFW8 View 3D Structure Click here
A0A1D6EZ76 View 3D Structure Click here
A0A1D6F8X2 View 3D Structure Click here
A0A1D6F9A2 View 3D Structure Click here
A0A1D6FV58 View 3D Structure Click here
A0A1D6H4I4 View 3D Structure Click here
A0A1D6H535 View 3D Structure Click here
A0A1D6HI30 View 3D Structure Click here
A0A1D6HKI7 View 3D Structure Click here
A0A1D6HWX2 View 3D Structure Click here
A0A1D6HWX3 View 3D Structure Click here
A0A1D6I2A6 View 3D Structure Click here
A0A1D6IHQ4 View 3D Structure Click here
A0A1D6J0T5 View 3D Structure Click here
A0A1D6J0T5 View 3D Structure Click here
A0A1D6JWB5 View 3D Structure Click here
A0A1D6JWB6 View 3D Structure Click here
A0A1D6KUK8 View 3D Structure Click here
A0A1D6KUL0 View 3D Structure Click here
A0A1D6L681 View 3D Structure Click here
A0A1D6LA05 View 3D Structure Click here
A0A1D6LA06 View 3D Structure Click here
A0A1D6LGK0 View 3D Structure Click here
A0A1D6LXJ2 View 3D Structure Click here
A0A1D6LXJ3 View 3D Structure Click here
A0A1D6NEV7 View 3D Structure Click here
A0A1D6NSJ1 View 3D Structure Click here
A0A1D6P2K7 View 3D Structure Click here
A0A1D6Q3I1 View 3D Structure Click here
A0A1D6Q3I1 View 3D Structure Click here
A0A1D6Q8H0 View 3D Structure Click here
A0A1D6QKU2 View 3D Structure Click here
B4F902 View 3D Structure Click here
B4FW79 View 3D Structure Click here
B6SVB5 View 3D Structure Click here
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B7ER53 View 3D Structure Click here
B7F8F0 View 3D Structure Click here
B7ZYU6 View 3D Structure Click here
B7ZYV3 View 3D Structure Click here
B9G7D2 View 3D Structure Click here
C0P6K2 View 3D Structure Click here
C0PCW2 View 3D Structure Click here
C4J4G8 View 3D Structure Click here
C6ZRW0 View 3D Structure Click here
D7F7K7 View 3D Structure Click here
F4IZV1 View 3D Structure Click here
I1J6Y3 View 3D Structure Click here
I1J751 View 3D Structure Click here
I1JCC0 View 3D Structure Click here
I1JFJ9 View 3D Structure Click here
I1JH80 View 3D Structure Click here
I1JL89 View 3D Structure Click here
I1JLM6 View 3D Structure Click here
I1JLU9 View 3D Structure Click here
I1KK20 View 3D Structure Click here
I1KK21 View 3D Structure Click here
I1KK88 View 3D Structure Click here
I1KL68 View 3D Structure Click here
I1KL69 View 3D Structure Click here
I1KQW2 View 3D Structure Click here
I1KQW5 View 3D Structure Click here
I1KQW6 View 3D Structure Click here
I1KQW7 View 3D Structure Click here
I1KR60 View 3D Structure Click here
I1KX47 View 3D Structure Click here
I1KX48 View 3D Structure Click here
I1KX49 View 3D Structure Click here
I1L3I1 View 3D Structure Click here
I1L4T9 View 3D Structure Click here
I1LDI3 View 3D Structure Click here
I1LIE6 View 3D Structure Click here
I1LM84 View 3D Structure Click here
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I1LML9 View 3D Structure Click here
I1LS36 View 3D Structure Click here
I1M233 View 3D Structure Click here
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I1M971 View 3D Structure Click here
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I1MB68 View 3D Structure Click here
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I1MTC9 View 3D Structure Click here
I1MVA5 View 3D Structure Click here
I1MX00 View 3D Structure Click here
I1MX68 View 3D Structure Click here
I1MZ63 View 3D Structure Click here
I1N1T0 View 3D Structure Click here
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I1N2R9 View 3D Structure Click here
I1N308 View 3D Structure Click here
K7K1C5 View 3D Structure Click here
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K7K423 View 3D Structure Click here
K7K921 View 3D Structure Click here
K7L1X7 View 3D Structure Click here
K7L5A1 View 3D Structure Click here
K7L993 View 3D Structure Click here
K7L999 View 3D Structure Click here
K7LD58 View 3D Structure Click here
K7LD59 View 3D Structure Click here
K7LF03 View 3D Structure Click here
K7LF04 View 3D Structure Click here
K7LIX3 View 3D Structure Click here
K7M5Z4 View 3D Structure Click here
K7MGU0 View 3D Structure Click here
K7MGU0 View 3D Structure Click here
K7MSD1 View 3D Structure Click here
K7N228 View 3D Structure Click here
K7TMA6 View 3D Structure Click here
K7UC72 View 3D Structure Click here
K7UKG6 View 3D Structure Click here
K7UQ35 View 3D Structure Click here
K7V8J9 View 3D Structure Click here
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O04533 View 3D Structure Click here
O04534 View 3D Structure Click here
O22833 View 3D Structure Click here
O22834 View 3D Structure Click here
O49445 View 3D Structure Click here
O80939 View 3D Structure Click here
O81291 View 3D Structure Click here
O81292 View 3D Structure Click here
P05046 View 3D Structure Click here
Q0DTB8 View 3D Structure Click here
Q0DY65 View 3D Structure Click here
Q0JBH6 View 3D Structure Click here
Q0JF65 View 3D Structure Click here
Q0JF98 View 3D Structure Click here
Q0JFE7 View 3D Structure Click here
Q2QN91 View 3D Structure Click here
Q337T2 View 3D Structure Click here
Q337T3 View 3D Structure Click here
Q3E884 View 3D Structure Click here
Q3EBA4 View 3D Structure Click here
Q54UD6 View 3D Structure Click here
Q5VNF2 View 3D Structure Click here
Q5ZBP8 View 3D Structure Click here
Q66GN2 View 3D Structure Click here
Q69PR8 View 3D Structure Click here
Q69TX5 View 3D Structure Click here
Q6ERH1 View 3D Structure Click here
Q6ERH5 View 3D Structure Click here
Q6H4T8 View 3D Structure Click here
Q6H7D2 View 3D Structure Click here
Q6K4S0 View 3D Structure Click here
Q6L4L2 View 3D Structure Click here
Q6YT57 View 3D Structure Click here
Q6YWI8 View 3D Structure Click here
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Q6YX20 View 3D Structure Click here
Q6YX21 View 3D Structure Click here
Q6Z8M4 View 3D Structure Click here
Q6ZFB3 View 3D Structure Click here
Q6ZFB7 View 3D Structure Click here
Q6ZIQ9 View 3D Structure Click here
Q6ZLL7 View 3D Structure Click here
Q6ZLL9 View 3D Structure Click here
Q7F226 View 3D Structure Click here
Q7FK82 View 3D Structure Click here
Q7X756 View 3D Structure Click here
Q7XIH7 View 3D Structure Click here
Q7XSW3 View 3D Structure Click here
Q7XUN6 View 3D Structure Click here
Q7XX91 View 3D Structure Click here
Q7XXF4 View 3D Structure Click here
Q7XXF5 View 3D Structure Click here
Q84ZI4 View 3D Structure Click here
Q84ZI6 View 3D Structure Click here
Q852A9 View 3D Structure Click here
Q8H4I6 View 3D Structure Click here
Q8LIP0 View 3D Structure Click here
Q8LIP2 View 3D Structure Click here
Q94DZ6 View 3D Structure Click here
Q96285 View 3D Structure Click here
Q9FG33 View 3D Structure Click here
Q9FHG4 View 3D Structure Click here
Q9FHX3 View 3D Structure Click here
Q9FIF0 View 3D Structure Click here
Q9FIF1 View 3D Structure Click here
Q9FJI4 View 3D Structure Click here
Q9LEA3 View 3D Structure Click here
Q9LFC7 View 3D Structure Click here
Q9LFH9 View 3D Structure Click here
Q9LJR2 View 3D Structure Click here
Q9LK72 View 3D Structure Click here
Q9LNN1 View 3D Structure Click here
Q9LNN2 View 3D Structure Click here
Q9LNN3 View 3D Structure Click here
Q9LNW6 View 3D Structure Click here
Q9LSL5 View 3D Structure Click here
Q9LSR8 View 3D Structure Click here
Q9LSR9 View 3D Structure Click here
Q9LSS0 View 3D Structure Click here
Q9LXA5 View 3D Structure Click here
Q9LYX1 View 3D Structure Click here
Q9LZF5 View 3D Structure Click here
Q9M020 View 3D Structure Click here
Q9M021 View 3D Structure Click here
Q9M1G3 View 3D Structure Click here
Q9M1G4 View 3D Structure Click here
Q9M1Z9 View 3D Structure Click here
Q9M1Z9 View 3D Structure Click here
Q9M2S4 View 3D Structure Click here
Q9M345 View 3D Structure Click here
Q9M395 View 3D Structure Click here
Q9M3D7 View 3D Structure Click here
Q9M3D8 View 3D Structure Click here
Q9M3E5 View 3D Structure Click here
Q9M9E0 View 3D Structure Click here
Q9S9U1 View 3D Structure Click here
Q9SR87 View 3D Structure Click here
Q9SZD5 View 3D Structure Click here
Q9ZR79 View 3D Structure Click here
Q9ZW09 View 3D Structure Click here
Q9ZW11 View 3D Structure Click here