Summary: Cytochrome b5-like Heme/Steroid binding domain
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Cytochrome b5". More...
Cytochrome b5 Edit Wikipedia article
| Cytochrome b5 | |
|---|---|
 | |
| Identifiers | |
| Symbol | CYB5A |
| Alt. symbols | CYB5 |
| NCBI gene | 1528 |
| HGNC | 2570 |
| OMIM | 250790 |
| PDB | 1JEX |
| RefSeq | NM_001914 |
| UniProt | P00167 |
| Other data | |
| Locus | Chr. 18 q23 |
| Cytochrome b5 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Cyt_B5 | ||||||||
| Pfam | PF00173 | ||||||||
| InterPro | IPR001199 | ||||||||
| PROSITE | PDOC00170 | ||||||||
| Membranome | 211 | ||||||||
| |||||||||
Cytochromes b5 are ubiquitous electron transport hemoproteins found in animals, plants, fungi and purple phototrophic bacteria. The microsomal and mitochondrial variants are membrane-bound, while bacterial and those from erythrocytes and other animal tissues are water-soluble. The family of cytochrome b5-like proteins includes (besides cytochrome b5 itself) hemoprotein domains covalently associated with other redox domains in flavocytochrome cytochrome b2 (L-lactate dehydrogenase; EC 1.1.2.3), sulfite oxidase (EC 1.8.3.1), plant and fungal nitrate reductases (EC 1.7.1.1, EC 1.7.1.2, EC 1.7.1.3), and plant and fungal cytochrome b5/acyl lipid desaturase fusion proteins.
Contents
Structure
3-D structures of a number of cytochrome b5 and yeast flavocytochrome b2 are known. The fold belongs to the α+β class, with two hydrophobic cores on each side of a β-sheet. The larger hydrophobic core constitutes the heme-binding pocket, closed off on each side by a pair of helices connected by a turn. The smaller hydrophobic core may have only a structural role and is formed by spatially close N-terminal and C-terminal segments. The two histidine residues provide the fifth and sixth heme ligands, and the propionate edge of the heme group lies at the opening of the heme crevice. Two isomers of cytochrome b5, referred to as the A (major) and B (minor) forms, differ by a 180° rotation of the heme about an axis defined by the α- and γ-meso carbons.
Cytochrome b5 in some biochemical reactions
EC 1.6.2.2 cytochrome-b5 reductase
- NADH + H+ + 2 ferricytochrome b5 → NAD+ + 2 ferrocytochrome b5
EC 1.10.2.1 L-ascorbate—cytochrome-b5 reductase
- L-ascorbate + ferricytochrome b5 → monodehydroascorbate + ferrocytochrome b5
EC 1.14.18.2 CMP-N-acetylneuraminate monooxygenase
- CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ → CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
EC 1.14.19.1 stearoyl-CoA 9-desaturase
- stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ → oleoyl-CoA + 2 ferricytochrome b5 + H2O
EC 1.14.19.3 linoleoyl-CoA 9-desaturase
- linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ → γ-linolenoyl-CoA + 2 ferricytochrome b5 + H2O
See also
References
- Lederer F (1994). "The cytochrome b5-fold: an adaptable module". Biochimie. 76 (7): 674–92. doi:10.1016/0300-9084(94)90144-9. PMID 7893819.
- Napier JA, Michaelson LV, Sayanova O (February 2003). "The role of cytochrome b5 fusion desaturases in the synthesis of polyunsaturated fatty acids". Prostaglandins, Leukotrienes, and Essential Fatty Acids. 68 (2): 135–43. doi:10.1016/S0952-3278(02)00263-6. PMID 12538077.
- Rivera M, Barillas-Mury C, Christensen KA, Little JW, Wells MA, Walker FA (December 1992). "Gene synthesis, bacterial expression, and 1H NMR spectroscopic studies of the rat outer mitochondrial membrane cytochrome b5". Biochemistry. 31 (48): 12233–40. doi:10.1021/bi00163a037. PMID 1333795.
- Schenkman JB, Jansson I (February 2003). "The many roles of cytochrome b5". Pharmacology & Therapeutics. 97 (2): 139–52. doi:10.1016/S0163-7258(02)00327-3. PMID 12559387.
External links
- PDB: 1B5A – Solution structure of rat cytochrome b5 (form A)
- PDB: 1B5B – Solution structure of rat cytochrome b5 (form B)
- PDB: 1CXY – X-ray structure of cytochrome b558 from Ectothiorhodospira vacuolata
- Online Mendelian Inheritance in Man (OMIM) 250790 – Methemoglobinemia due to deficiency of cytochrome b5
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Cytochrome b5-like Heme/Steroid binding domain Provide feedback
This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors such as O00264 [1,2]. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.
Literature references
-
Gerdes D, Wehling M, Leube B, Falkenstein E; , Biol Chem 1998;379:907-911.: Cloning and tissue expression of two putative steroid membrane receptors. PUBMED:9705155 EPMC:9705155
-
Meyer C, Schmid R, Scriba PC, Wehling M; , Eur J Biochem 1996;239:726-731.: Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes. PUBMED:8774719 EPMC:8774719
-
Mifsud W, Bateman A; , Genome Biol 2002;3:0-0.: Membrane-bound progesterone receptors contain a cytochrome b5-like ligand-binding domain. PUBMED:12537557 EPMC:12537557
External database links
| HOMSTRAD: | cytb |
| PRINTS: | PR00406 |
| PROSITE: | PDOC00170 |
| SCOP: | 3b5c |
This tab holds annotation information from the InterPro database.
InterPro entry IPR001199
Cytochrome b5 is a membrane-bound hemoprotein which acts as an electron carrier for several membrane-bound oxygenases [PUBMED:2752049]. There are two homologous forms of b5, one found in microsomes and one found in the outer membrane of mitochondria. Two conserved histidine residues serve as axial ligands for the heme group. The structure of a number of oxidoreductases consists of the juxtaposition of a heme-binding domain homologous to that of b5 and either a flavodehydrogenase or a molybdopterin domain. These enzymes are:
- Lactate dehydrogenase (EC 1.1.2.3) [PUBMED:3004948], an enzyme that consists of a flavodehydrogenase domain and a heme-binding domain called cytochrome b2.
- Nitrate reductase (EC 1.7.1.-), a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [PUBMED:3393528]. Consists of a molybdopterin domain, a heme-binding domain called cytochrome b557, as well as a cytochrome reductase domain.
- Sulfite oxidase (EC 1.8.3.1) [PUBMED:510290], which catalyzes the terminal reaction in the oxidative degradation of sulfur-containing amino acids. Also consists of a molybdopterin domain and a heme-binding domain.
- Yeast acyl-CoA desaturase 1 (EC 1.14.19.1; gene OLE1). This enzyme contains a C-terminal heme-binding domain.
- Yeast Scs7 (YMR272c), a sphingolipid alpha-hydroxylase.
Proteins containing a cytochrome b5-like domain also include:
- TU-36B, a Drosophila muscle protein of unknown function [PUBMED:2549511].
- Fission yeast hypothetical protein SpAC1F12.10c (C1F12.10c).
- Yeast Irc21 (YMR073c), a putative protein with unknown function.
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
Loading domain graphics...
Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
| Seed (82) |
Full (21983) |
Representative proteomes | UniProt (36918) |
NCBI (42810) |
Meta (243) |
||||
|---|---|---|---|---|---|---|---|---|---|
| RP15 (3322) |
RP35 (9152) |
RP55 (14609) |
RP75 (21319) |
||||||
| Jalview | |||||||||
| HTML | |||||||||
| PP/heatmap | 1 | ||||||||
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
— not available.
Format an alignment
Download options
We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
| Seed (82) |
Full (21983) |
Representative proteomes | UniProt (36918) |
NCBI (42810) |
Meta (243) |
||||
|---|---|---|---|---|---|---|---|---|---|
| RP15 (3322) |
RP35 (9152) |
RP55 (14609) |
RP75 (21319) |
||||||
| Raw Stockholm | |||||||||
| Gzipped | |||||||||
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
| Seed source: | Bateman A |
| Previous IDs: | heme_1; |
| Type: | Domain |
| Sequence Ontology: | SO:0000417 |
| Author: |
Bateman A 
|
| Number in seed: | 82 |
| Number in full: | 21983 |
| Average length of the domain: | 81.70 aa |
| Average identity of full alignment: | 26 % |
| Average coverage of the sequence by the domain: | 15.83 % |
HMM information
| HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
|
||||||||||||
| Model details: |
|
||||||||||||
| Model length: | 74 | ||||||||||||
| Family (HMM) version: | 29 | ||||||||||||
| Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
HideWeight segments by...
Change the size of the sunburst
Colour assignments
Archea
|
Eukaryota
|
Bacteria
|
Other sequences
|
Viruses
|
Unclassified
|
Viroids
|
Unclassified sequence
|
Selections
Align selected sequences to HMM
Generate a FASTA-format file
Clear selection
This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Loading...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
Interactions
Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Cyt-b5 domain has been found. There are 102 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
Loading structure mapping...
