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46  structures 1245  species 0  interactions 4994  sequences 147  architectures

Family: Glyco_hydro_19 (PF00182)

Summary: Chitinase class I

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This is the Wikipedia entry entitled "Glycoside hydrolase family 19". More...

Glycoside hydrolase family 19 Edit Wikipedia article

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Chitinase class I Provide feedback

No Pfam abstract.

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000726

O-Glycosyl hydrolases ( EC ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ PUBMED:7624375 , PUBMED:8535779 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 19 CAZY comprises enzymes with only one known activity; chitinase ( EC ).

Chitinases [ PUBMED:1516675 ] are enzymes that catalyse the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Chitinases belong to glycoside hydrolase families 18 or 19 [ PUBMED:1747104 ]. Chitinases of family 19 (also known as classes I, II and IV) are enzymes from plants that function in the defence against fungal and insect pathogens by destroying their chitin-containing cell wall. Some family 19 chitinases are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I, but they are smaller in size due to certain deletions.

Despite any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the procaryotic/eucaryotic divergence [ PUBMED:10957628 , PUBMED:12369923 , PUBMED:10906956 , PUBMED:10906957 , PUBMED:9723170 , PUBMED:8564539 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Lysozyme (CL0037), which has the following description:

Barley chitinase, bacterial chitosanase, and lysozymes from phage and animals all hydrolyse related polysaccharides. The proteins little amino-acid similarity, but have a structurally invariant core consisting of two helices and a three-stranded beta-sheet which form the substrate-binding and catalytic cleft [1].

The clan contains the following 21 members:

Destabilase DUF3218 Glucosaminidase Glyco_hydro_108 Glyco_hydro_19 Glyco_hydro_46 Glyco_hydro_80 Lys Lysozyme_like Peptidase_U40 Pesticin Phage_lysozyme Phage_lysozyme2 SLT SLT_2 SLT_3 SLT_4 TraH_2 Transgly Transglycosylas Utp11

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(6)
Full
(4994)
Representative proteomes UniProt
(17151)
RP15
(678)
RP35
(2494)
RP55
(4437)
RP75
(6737)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(6)
Full
(4994)
Representative proteomes UniProt
(17151)
RP15
(678)
RP35
(2494)
RP55
(4437)
RP75
(6737)
Alignment:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(6)
Full
(4994)
Representative proteomes UniProt
(17151)
RP15
(678)
RP35
(2494)
RP55
(4437)
RP75
(6737)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: chitinase_1;
Type: Domain
Sequence Ontology: SO:0000417
Author: Finn RD
Number in seed: 6
Number in full: 4994
Average length of the domain: 158.60 aa
Average identity of full alignment: 30 %
Average coverage of the sequence by the domain: 58.56 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.5 25.5
Trusted cut-off 25.5 25.5
Noise cut-off 25.4 25.4
Model length: 232
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_19 domain has been found. There are 46 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0XWU1 View 3D Structure Click here
A0A0R0HQE5 View 3D Structure Click here
A0A0R0HSB0 View 3D Structure Click here
A0A0R0HSB0 View 3D Structure Click here
A0A1D6E7B3 View 3D Structure Click here
A0A1D6E7B3 View 3D Structure Click here
A0A1D6GWN1 View 3D Structure Click here
A0A1D6GWN1 View 3D Structure Click here
A0A1D6GWN3 View 3D Structure Click here
A0A1D6JS63 View 3D Structure Click here
A0A1D6K7T5 View 3D Structure Click here
A0A1D6LKX7 View 3D Structure Click here
A0A1D6LMS1 View 3D Structure Click here
A0A1D6LMS5 View 3D Structure Click here
A0A1D6PVW4 View 3D Structure Click here
A0A1P8AME8 View 3D Structure Click here
A0A1P8AWZ7 View 3D Structure Click here
A0A1X7YIJ7 View 3D Structure Click here
B4F9D5 View 3D Structure Click here
B4FTS6 View 3D Structure Click here
B4FTS6 View 3D Structure Click here
B4G1H3 View 3D Structure Click here
B6TR38 View 3D Structure Click here
B6TT00 View 3D Structure Click here
B6TT00 View 3D Structure Click here
C0P3M6 View 3D Structure Click here
C0P451 View 3D Structure Click here
C0P451 View 3D Structure Click here
C0PKN5 View 3D Structure Click here
C0PKN5 View 3D Structure Click here
C6T7J9 View 3D Structure Click here
C6T7J9 View 3D Structure Click here
C6TNB0 View 3D Structure Click here
F4JBK4 View 3D Structure Click here
F4JBK4 View 3D Structure Click here
I1J8E1 View 3D Structure Click here
I1JBA3 View 3D Structure Click here
I1KWS8 View 3D Structure Click here
I1L0T4 View 3D Structure Click here
I1M587 View 3D Structure Click here
I1M587 View 3D Structure Click here
I1MGH1 View 3D Structure Click here
I1MMY2 View 3D Structure Click here
I1N4Z6 View 3D Structure Click here
K7LJA7 View 3D Structure Click here
K7LT36 View 3D Structure Click here
K7VNG7 View 3D Structure Click here
K7VXP1 View 3D Structure Click here
N1NTG0 View 3D Structure Click here
O04138 View 3D Structure Click here
O04138 View 3D Structure Click here
O16512 View 3D Structure Click here
O22841 View 3D Structure Click here
O22841 View 3D Structure Click here
O22842 View 3D Structure Click here
O22842 View 3D Structure Click here
O24598 View 3D Structure Click here
O24603 View 3D Structure Click here
O24654 View 3D Structure Click here
O24658 View 3D Structure Click here
O24658 View 3D Structure Click here
P19171 View 3D Structure Click here
P24626 View 3D Structure Click here
P25765 View 3D Structure Click here
P29022 View 3D Structure Click here
P29022 View 3D Structure Click here
P29023 View 3D Structure Click here
P29023 View 3D Structure Click here
Q0J0L1 View 3D Structure Click here
Q10S66 View 3D Structure Click here
Q42993 View 3D Structure Click here
Q5NB11 View 3D Structure Click here
Q65ZB0 View 3D Structure Click here
Q688M5 View 3D Structure Click here
Q6AT95 View 3D Structure Click here
Q6K8R2 View 3D Structure Click here
Q6K8R2 View 3D Structure Click here
Q7DNA1 View 3D Structure Click here
Q7XCK6 View 3D Structure Click here
Q7Y1Z0 View 3D Structure Click here
Q7Y1Z0 View 3D Structure Click here
Q7Y1Z1 View 3D Structure Click here
Q84QQ7 View 3D Structure Click here
Q9LSP9 View 3D Structure Click here
Q9M2U5 View 3D Structure Click here
Q9M2U5 View 3D Structure Click here
Q9MA41 View 3D Structure Click here
Q9SDY6 View 3D Structure Click here
Q9ZSI6 View 3D Structure Click here