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36  structures 1355  species 0  interactions 10675  sequences 337  architectures

Family: PI-PLC-Y (PF00387)

Summary: Phosphatidylinositol-specific phospholipase C, Y domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Phosphatidylinositol-specific phospholipase C, Y domain Provide feedback

This associates with PF00388 to form a single structural unit.

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001711

Phosphatidylinositol-specific phospholipase C ( EC ), an eukaryotic intracellular enzyme, plays an important role in signal transduction processes [ PUBMED:1849017 ] (see INTERPRO ). It catalyzes the hydrolysis of 1-phosphatidyl-D-myo-inositol-3,4,5-triphosphate into the second messenger molecules diacylglycerol and inositol-1,4,5-triphosphate. This catalytic process is tightly regulated by reversible phosphorylation and binding of regulatory proteins [ PUBMED:1419362 , PUBMED:1319994 , PUBMED:1335185 ].

In mammals, there are at least 6 different isoforms of PI-PLC, they differ in their domain structure, their regulation, and their tissue distribution. Lower eukaryotes also possess multiple isoforms of PI-PLC.

All eukaryotic PI-PLCs contain two regions of homology, sometimes referred to as 'X-box' (see INTERPRO ) and 'Y-box'. The order of these two regions is always the same (NH2-X-Y-COOH), but the spacing is variable. In most isoforms, the distance between these two regions is only 50-100 residues but in the gamma isoforms one PH domain, two SH2 domains, and one SH3 domain are inserted between the two PLC-specific domains. The two conserved regions have been shown to be important for the catalytic activity. At the C-terminal of the Y-box, there is a C2 domain (see INTERPRO ) possibly involved in Ca-dependent membrane attachment.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PLC (CL0384), which has the following description:

Superfamily consists of Glycerophosphoryl diester phosphodiesterase and phosphatidylinositol-specific phospholipase C families.

The clan contains the following 4 members:

GDPD GDPD_2 PI-PLC-X PI-PLC-Y

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(304)
Full
(10675)
Representative proteomes UniProt
(18671)
RP15
(1331)
RP35
(3853)
RP55
(8706)
RP75
(11775)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(304)
Full
(10675)
Representative proteomes UniProt
(18671)
RP15
(1331)
RP35
(3853)
RP55
(8706)
RP75
(11775)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(304)
Full
(10675)
Representative proteomes UniProt
(18671)
RP15
(1331)
RP35
(3853)
RP55
(8706)
RP75
(11775)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD
Number in seed: 304
Number in full: 10675
Average length of the domain: 111.70 aa
Average identity of full alignment: 41 %
Average coverage of the sequence by the domain: 11.34 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.8 28.8
Trusted cut-off 29.0 28.8
Noise cut-off 28.3 28.6
Model length: 115
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the PI-PLC-Y domain has been found. There are 36 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A096MJW8 View 3D Structure Click here
A0A0G2KNE7 View 3D Structure Click here
A0A0R0GIK6 View 3D Structure Click here
A0A0R0GLL7 View 3D Structure Click here
A0A0R0HUL4 View 3D Structure Click here
A0A0R0L9Z2 View 3D Structure Click here
A0A0R4IVA6 View 3D Structure Click here
A0A1D6F512 View 3D Structure Click here
A0A1D6GXH6 View 3D Structure Click here
A0A1D6GXI7 View 3D Structure Click here
A0A1D6JZ59 View 3D Structure Click here
A0A1D8PRE4 View 3D Structure Click here
A0A2R8PZX9 View 3D Structure Click here
A0A2R8QLB0 View 3D Structure Click here
A0A2R8RJ32 View 3D Structure Click here
A0A2R8RVV2 View 3D Structure Click here
A2AP18 View 3D Structure Click here
A3KGF7 View 3D Structure Click here
A4I027 View 3D Structure Click here
A4I5X4 View 3D Structure Click here
A4IAQ5 View 3D Structure Click here
A5D6R3 View 3D Structure Click here
B9F7R4 View 3D Structure Click here
D4A978 View 3D Structure Click here
D4AAX6 View 3D Structure Click here
E7F5Y6 View 3D Structure Click here
E7F646 View 3D Structure Click here
E7F8G4 View 3D Structure Click here
F1M324 View 3D Structure Click here
F1Q5G9 View 3D Structure Click here
F1QAP6 View 3D Structure Click here
F1QJ13 View 3D Structure Click here
F1QLX2 View 3D Structure Click here
F1QS56 View 3D Structure Click here
F1QS65 View 3D Structure Click here
F1R8S3 View 3D Structure Click here
F1RET1 View 3D Structure Click here
G5EBH0 View 3D Structure Click here
G5EFI8 View 3D Structure Click here
I1JI88 View 3D Structure Click here
I1JI89 View 3D Structure Click here
I1JI90 View 3D Structure Click here
I1LMR9 View 3D Structure Click here
I1M7T8 View 3D Structure Click here
I1MBB2 View 3D Structure Click here
I1MZ21 View 3D Structure Click here
I1MZ23 View 3D Structure Click here
K7LRR0 View 3D Structure Click here
K7MPJ5 View 3D Structure Click here
K7VZB3 View 3D Structure Click here
O75038 View 3D Structure Click here
O89040 View 3D Structure Click here
P10686 View 3D Structure Click here
P10687 View 3D Structure Click here
P10688 View 3D Structure Click here
P13217 View 3D Structure Click here
P16885 View 3D Structure Click here
P19174 View 3D Structure Click here
P24135 View 3D Structure Click here
P25455 View 3D Structure Click here
P32383 View 3D Structure Click here
P40977 View 3D Structure Click here
P51178 View 3D Structure Click here
P51432 View 3D Structure Click here
Q00722 View 3D Structure Click here
Q01970 View 3D Structure Click here
Q02158 View 3D Structure Click here
Q15111 View 3D Structure Click here
Q15147 View 3D Structure Click here
Q21754 View 3D Structure Click here
Q22070 View 3D Structure Click here
Q2QNK1 View 3D Structure Click here
Q39032 View 3D Structure Click here
Q39033 View 3D Structure Click here
Q3USB7 View 3D Structure Click here
Q43443 View 3D Structure Click here
Q4DUP6 View 3D Structure Click here
Q4KWH5 View 3D Structure Click here
Q4KWH8 View 3D Structure Click here
Q56W08 View 3D Structure Click here
Q5FX52 View 3D Structure Click here
Q62077 View 3D Structure Click here
Q62688 View 3D Structure Click here
Q62711 View 3D Structure Click here
Q6NMA7 View 3D Structure Click here
Q6Z3Y9 View 3D Structure Click here
Q75IL8 View 3D Structure Click here
Q86YW0 View 3D Structure Click here
Q8CIH5 View 3D Structure Click here
Q8GV43 View 3D Structure Click here
Q8IA75 View 3D Structure Click here
Q8IJR0 View 3D Structure Click here
Q8K2J0 View 3D Structure Click here
Q8K394 View 3D Structure Click here
Q8K3R3 View 3D Structure Click here
Q8K4D7 View 3D Structure Click here
Q8K4S1 View 3D Structure Click here
Q8N3E9 View 3D Structure Click here
Q8R3B1 View 3D Structure Click here
Q91UZ1 View 3D Structure Click here
Q944C1 View 3D Structure Click here
Q944C2 View 3D Structure Click here
Q99JE6 View 3D Structure Click here
Q99P84 View 3D Structure Click here
Q9BRC7 View 3D Structure Click here
Q9LY51 View 3D Structure Click here
Q9NQ66 View 3D Structure Click here
Q9P212 View 3D Structure Click here
Q9QW07 View 3D Structure Click here
Q9STZ3 View 3D Structure Click here
Q9UPR0 View 3D Structure Click here
Q9VXH3 View 3D Structure Click here
Q9XWB7 View 3D Structure Click here
Q9Z1B3 View 3D Structure Click here