Summary: FHA domain
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This is the Wikipedia entry entitled "Forkhead-associated domain". More...
Forkhead-associated domain Edit Wikipedia article
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FHA domain Provide feedback
The FHA (Forkhead-associated) domain is a phosphopeptide binding motif [2].
Literature references
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Hofmann K, Bucher, P; , Trends Biochem Sci 1995;20:347-349.: The FHA domain: a putative nuclear signalling domain found in protein kinases and transcription factors. PUBMED:7482699 EPMC:7482699
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Durocher D, Henckel J, Fersht AR, Jackson SP; , Mol Cell. 1999;4:387-394.: The FHA domain is a modular phosphopeptide recognition motif. PUBMED:10518219 EPMC:10518219
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Pallen M, Chaudhuri R, Khan A; , Trends Microbiol 2002;10:556-563.: Bacterial FHA domains: neglected players in the phospho-threonine signalling game? PUBMED:12564991 EPMC:12564991
Internal database links
SCOOP: | FHA_2 Pkinase_fungal Yop-YscD_cpl |
Similarity to PfamA using HHSearch: | Yop-YscD_cpl FHA_2 |
External database links
HOMSTRAD: | FHA |
PROSITE: | PDOC50006 |
SCOP: | 1qu5 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR000253
The forkhead-associated (FHA) domain [PUBMED:7482699] is a phosphopeptide recognition domain found in many regulatory proteins. It displays specificity for phosphothreonine-containing epitopes but will also recognise phosphotyrosine with relatively high affinity. It spans approximately 80-100 amino acid residues folded into an 11-stranded beta sandwich, which sometimes contain small helical insertions between the loops connecting the strands [PUBMED:11911881].
To date, genes encoding FHA-containing proteins have been identified in eubacterial and eukaryotic but not archaeal genomes. The domain is present in a diverse range of proteins, such as kinases, phosphatases, kinesins, transcription factors, RNA-binding proteins and metabolic enzymes which partake in many different cellular processes - DNA repair, signal transduction, vesicular transport and protein degradation are just a few examples.
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | protein binding (GO:0005515) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan SMAD-FHA (CL0357), which has the following description:
Superfamily members carry a few short helices inserted in loops within the 11 strands in 2 sheets (greek-key) of the parent fold.
The clan contains the following 9 members:
FHA FHA_2 FtsK_SpoIIIE_N IRF-3 MH2 MU2_FHA Pellino PrgH Yop-YscD_cplAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (195) |
Full (33008) |
Representative proteomes | UniProt (86778) |
NCBI (152348) |
Meta (968) |
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RP15 (5078) |
RP35 (14579) |
RP55 (27343) |
RP75 (42659) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (195) |
Full (33008) |
Representative proteomes | UniProt (86778) |
NCBI (152348) |
Meta (968) |
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---|---|---|---|---|---|---|---|---|---|
RP15 (5078) |
RP35 (14579) |
RP55 (27343) |
RP75 (42659) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Prosite |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Finn RD |
Number in seed: | 195 |
Number in full: | 33008 |
Average length of the domain: | 72.00 aa |
Average identity of full alignment: | 25 % |
Average coverage of the sequence by the domain: | 10.74 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 69 | ||||||||||||
Family (HMM) version: | 27 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Selections
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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
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Interactions
There are 11 interactions for this family. More...
E1_dh Trans_reg_C Trans_reg_C BTAD Pkinase hNIFK_binding FHA PH_11 BTAD ArfGap PkinaseStructures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the FHA domain has been found. There are 159 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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