Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
213  structures 8448  species 0  interactions 20996  sequences 661  architectures

Family: BRCT (PF00533)

Summary: BRCA1 C Terminus (BRCT) domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "BRCT domain". More...

BRCT domain Edit Wikipedia article

BRCA1 C Terminus (BRCT) domain
PDB 1cdz EBI.jpg
Structure of an XRCC1 BRCT domain.[1]
Identifiers
SymbolBRCT
PfamPF00533
InterProIPR001357
SCOPe1cdz / SUPFAM
CDDcd00027

BRCA1 C Terminus (BRCT) domain is a family of evolutionarily related proteins. It is named after the C-terminal domain of BRCA1, a DNA-repair protein that serves as a marker of breast cancer susceptibility.

The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage,[2] for example as found in the breast cancer DNA-repair protein BRCA1. The domain is an approximately 100 amino acid tandem repeat, which appears to act as a phospho-protein binding domain.[3]

Examples

Human proteins containing this domain include:

References

  1. ^ Zhang X, Moréra S, Bates PA, et al. (November 1998). "Structure of an XRCC1 BRCT domain: a new protein-protein interaction module". EMBO J. 17 (21): 6404–11. doi:10.1093/emboj/17.21.6404. PMC 1170965. PMID 9799248.
  2. ^ Bork P, Hofmann K, Koonin EV, Bucher P, Neuwald AF, Altschul SF (1997). "A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins". FASEB J. 11 (1): 68–76. PMID 9034168.
  3. ^ Yu X, Chini CC, He M, Mer G, Chen J (2003). "The BRCT domain is a phospho-protein binding domain". Science. 302 (5645): 639–642. doi:10.1126/science.1088753. PMID 14576433.

External links

This article incorporates text from the public domain Pfam and InterPro: IPR001357


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

BRCA1 C Terminus (BRCT) domain Provide feedback

The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage. The BRCT domain of XRCC1 forms a homodimer in the crystal structure. This suggests that pairs of BRCT domains associate as homo- or heterodimers. BRCT domains are often found as tandem-repeat pairs [2]. Structures of the BRCA1 BRCT domains revealed a basis for a widely utilised head-to-tail BRCT-BRCT oligomerisation mode [3]. This conserved tandem BRCT architecture facilitates formation of the canonical BRCT phospho-peptide interaction cleft at a groove between the BRCT domains. Disease associated missense and nonsense mutations in the BRCA1 BRCT domains disrupt peptide binding by directly occluding this peptide binding groove, or by disrupting key conserved BRCT core folding determinants [5].

Literature references

  1. Koonin EV, Altschul SF, Bork P; , Nature Genet 1996;13:266-268.: BRCA1 protein products ...Functional motifs... PUBMED:8673121 EPMC:8673121

  2. Glover JN, Williams RS, Lee MS; , Trends Biochem Sci. 2004;29:579-585.: Interactions between BRCT repeats and phosphoproteins: tangled up in two. PUBMED:15501676 EPMC:15501676

  3. Williams RS, Green R, Glover JN; , Nat Struct Biol. 2001;8:838-842.: Crystal structure of the BRCT repeat region from the breast cancer-associated protein BRCA1. PUBMED:11573086 EPMC:11573086

  4. Williams RS, Lee MS, Hau DD, Glover JN; , Nat Struct Mol Biol. 2004;11:519-525.: Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1. PUBMED:15133503 EPMC:15133503

  5. Williams RS, Chasman DI, Hau DD, Hui B, Lau AY, Glover JN; , J Biol Chem. 2003;278:53007-53016.: Detection of protein folding defects caused by BRCA1-BRCT truncation and missense mutations. PUBMED:14534301 EPMC:14534301


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001357

The breast cancer susceptibility gene contains at its C terminus two copies of a conserved domain that was named BRCT for BRCA1 C terminus. This domain of about 95 amino acids is found in a large variety of proteins involved in DNA repair, recombination and cell cycle control [ PUBMED:8673121 , PUBMED:9034168 , PUBMED:9000507 ]. The BRCT domain is not limited to the C-terminal of protein sequences and can be found in multiple copies or in a single copy as in RAP1 and TdT. BRCT domains are often found as tandem-repeat pairs [ PUBMED:15501676 ]. Some data [ PUBMED:9799248 ] indicate that the BRCT domain functions as a protein-protein interaction module.

The structure of the first of the two C-terminal BRCT domains of the human DNA repair protein XRCC1 has recently been determined by X-ray crystallography [ PUBMED:9799248 ].

Structures of the BRCA1 BRCT domains revealed a basis for a widely utilised head-to-tail BRCT-BRCT oligomerisation mode [ PUBMED:11573086 ]. This conserved tandem BRCT architecture facilitates formation of the canonical BRCT phospho-peptide interaction cleft at a groove between the BRCT domains. BRCT domains disrupt peptide binding by directly occluding this peptide binding groove, or by disrupting key conserved BRCT core folding determinants [ PUBMED:15133503 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan BRCT-like (CL0459), which has the following description:

The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage.

The clan contains the following 9 members:

BRCT BRCT_2 BRCT_3 Dfp1_Him1_M LIG3_BRCT NIBRIN_BRCT_II PTCB-BRCT RTT107_BRCT_5 RTT107_BRCT_6

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(80)
Full
(20996)
Representative proteomes UniProt
(66708)
RP15
(3239)
RP35
(9472)
RP55
(18886)
RP75
(28949)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(80)
Full
(20996)
Representative proteomes UniProt
(66708)
RP15
(3239)
RP35
(9472)
RP55
(18886)
RP75
(28949)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(80)
Full
(20996)
Representative proteomes UniProt
(66708)
RP15
(3239)
RP35
(9472)
RP55
(18886)
RP75
(28949)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: [3]
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 80
Number in full: 20996
Average length of the domain: 78.70 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 10.94 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.2 21.2
Trusted cut-off 21.2 21.2
Noise cut-off 21.1 21.1
Model length: 79
Family (HMM) version: 28
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BRCT domain has been found. There are 213 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0WNT7 View 3D Structure Click here
A0A0P0XAV3 View 3D Structure Click here
A0A0P0Y2X9 View 3D Structure Click here
A0A0R0EYR1 View 3D Structure Click here
A0A0R0F5G2 View 3D Structure Click here
A0A0R0FU99 View 3D Structure Click here
A0A0R0FUK3 View 3D Structure Click here
A0A0R0G752 View 3D Structure Click here
A0A0R0HW95 View 3D Structure Click here
A0A0R0KQH6 View 3D Structure Click here
A0A0R4IKD5 View 3D Structure Click here
A0A0R4INB3 View 3D Structure Click here
A0A1D6E647 View 3D Structure Click here
A0A1D6EH49 View 3D Structure Click here
A0A1D6F6V5 View 3D Structure Click here
A0A1D6HC20 View 3D Structure Click here
A0A1D6M367 View 3D Structure Click here
A0A1D6PTK9 View 3D Structure Click here
A0A1D8PHI0 View 3D Structure Click here
A0A1D8PI44 View 3D Structure Click here
A0A1D8PNH8 View 3D Structure Click here
A0A1P8AWF3 View 3D Structure Click here
A0A2R8RVN3 View 3D Structure Click here
A0A2R8RVN3 View 3D Structure Click here
A0A2R8RVN3 View 3D Structure Click here
A0A2R8RVN3 View 3D Structure Click here
A3EWL3 View 3D Structure Click here
A4IAJ5 View 3D Structure Click here
B3H6L5 View 3D Structure Click here
B4G1L6 View 3D Structure Click here
B7EHL9 View 3D Structure Click here
D3ZFT1 View 3D Structure Click here
D3ZRG0 View 3D Structure Click here
D3ZRG0 View 3D Structure Click here
D3ZRG0 View 3D Structure Click here
D3ZSX6 View 3D Structure Click here
D3ZUD0 View 3D Structure Click here
D4A0U6 View 3D Structure Click here
E1JH39 View 3D Structure Click here
E9PT58 View 3D Structure Click here
F1Q5P6 View 3D Structure Click here
F1Q9E8 View 3D Structure Click here
F1QXI2 View 3D Structure Click here
F4I443 View 3D Structure Click here
F8W5H6 View 3D Structure Click here
I1JP21 View 3D Structure Click here
I1KFH0 View 3D Structure Click here
I1KWR8 View 3D Structure Click here
I1LRM8 View 3D Structure Click here
I1M5N0 View 3D Structure Click here
I1N9T1 View 3D Structure Click here
K7K5X0 View 3D Structure Click here
K7KER9 View 3D Structure Click here
K7KER9 View 3D Structure Click here
K7LQ69 View 3D Structure Click here
K7M147 View 3D Structure Click here
O04251 View 3D Structure Click here
O14079 View 3D Structure Click here
O44844 View 3D Structure Click here
O45463 View 3D Structure Click here
O60182 View 3D Structure Click here
O74161 View 3D Structure Click here
O96271 View 3D Structure Click here
P04053 View 3D Structure Click here
P09838 View 3D Structure Click here
P09874 View 3D Structure Click here
P11103 View 3D Structure Click here
P14737 View 3D Structure Click here
P15042 View 3D Structure Click here
P18887 View 3D Structure Click here
P18887 View 3D Structure Click here
P27008 View 3D Structure Click here
P32372 View 3D Structure Click here
P32372 View 3D Structure Click here
P35251 View 3D Structure Click here
P35600 View 3D Structure Click here
P35601 View 3D Structure Click here
P35875 View 3D Structure Click here
P38398 View 3D Structure Click here
P38398 View 3D Structure Click here
P38630 View 3D Structure Click here
P38850 View 3D Structure Click here
P38850 View 3D Structure Click here
P38850 View 3D Structure Click here
P41882 View 3D Structure Click here
P47027 View 3D Structure Click here
P48754 View 3D Structure Click here
P48754 View 3D Structure Click here
P49917 View 3D Structure Click here
P49917 View 3D Structure Click here
P52496 View 3D Structure Click here
P87074 View 3D Structure Click here
P9WNV1 View 3D Structure Click here
Q00IB6 View 3D Structure Click here
Q03254 View 3D Structure Click here
Q07139 View 3D Structure Click here
Q08387 View 3D Structure Click here
Q08387 View 3D Structure Click here
Q0DGU0 View 3D Structure Click here
Q10337 View 3D Structure Click here
Q10337 View 3D Structure Click here
Q10ML5 View 3D Structure Click here
Q12114 View 3D Structure Click here
Q1ZXG5 View 3D Structure Click here
Q20129 View 3D Structure Click here
Q20129 View 3D Structure Click here
Q20129 View 3D Structure Click here
Q24JK4 View 3D Structure Click here
Q2G1Y0 View 3D Structure Click here
Q2R2B4 View 3D Structure Click here
Q4CR37 View 3D Structure Click here
Q4D9Y0 View 3D Structure Click here
Q4DQZ4 View 3D Structure Click here
Q4DSB5 View 3D Structure Click here
Q4E4D2 View 3D Structure Click here
Q54CR9 View 3D Structure Click here
Q54H15 View 3D Structure Click here
Q54HY5 View 3D Structure Click here
Q54IJ8 View 3D Structure Click here
Q54LN0 View 3D Structure Click here
Q54MH9 View 3D Structure Click here
Q54Q95 View 3D Structure Click here
Q54QM4 View 3D Structure Click here
Q54RH5 View 3D Structure Click here
Q54X69 View 3D Structure Click here
Q54X69 View 3D Structure Click here
Q5ALY1 View 3D Structure Click here
Q5ALY1 View 3D Structure Click here
Q5RHR0 View 3D Structure Click here
Q5VQ75 View 3D Structure Click here
Q5XIY8 View 3D Structure Click here
Q60596 View 3D Structure Click here
Q6K8Y2 View 3D Structure Click here
Q6NZQ4 View 3D Structure Click here
Q6ZQF0 View 3D Structure Click here
Q6ZQF0 View 3D Structure Click here
Q6ZQF0 View 3D Structure Click here
Q6ZQF0 View 3D Structure Click here
Q6ZW49 View 3D Structure Click here
Q6ZW49 View 3D Structure Click here
Q6ZW49 View 3D Structure Click here
Q75JA4 View 3D Structure Click here
Q7EYV7 View 3D Structure Click here
Q7JKP6 View 3D Structure Click here
Q7XPZ2 View 3D Structure Click here
Q86H44 View 3D Structure Click here
Q86KB4 View 3D Structure Click here
Q8BTF7 View 3D Structure Click here
Q8BTF7 View 3D Structure Click here
Q8I7C6 View 3D Structure Click here
Q8RXD4 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q92547 View 3D Structure Click here
Q93161 View 3D Structure Click here
Q95QG8 View 3D Structure Click here
Q9C587 View 3D Structure Click here
Q9ESZ0 View 3D Structure Click here
Q9H8V3 View 3D Structure Click here
Q9QZ41 View 3D Structure Click here
Q9SWB4 View 3D Structure Click here
Q9U370 View 3D Structure Click here
Q9U370 View 3D Structure Click here
Q9U7C9 View 3D Structure Click here
Q9UKK3 View 3D Structure Click here
Q9VT40 View 3D Structure Click here
Q9VUB7 View 3D Structure Click here
Q9VUB7 View 3D Structure Click here
Q9VY97 View 3D Structure Click here
Q9VY97 View 3D Structure Click here
Q9VY97 View 3D Structure Click here
Q9VY97 View 3D Structure Click here
Q9W147 View 3D Structure Click here
Q9XV62 View 3D Structure Click here
Q9Y095 View 3D Structure Click here
Q9ZP54 View 3D Structure Click here