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1037  structures 8381  species 0  interactions 46928  sequences 382  architectures

Family: AhpC-TSA (PF00578)

Summary: AhpC/TSA family

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

AhpC/TSA family Provide feedback

This family contains proteins related to alkyl hydroperoxide reductase (AhpC) and thiol specific antioxidant (TSA).

Literature references

  1. Chae HZ, Robison K, Poole LB, Church G, Storz G, Rhee SG; , Proc Natl Acad Sci U S A 1994;91:7017-7021.: Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes. PUBMED:8041738 EPMC:8041738


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000866

Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes that also control cytokine-induced peroxide levels which mediate signal transduction in mammalian cells. Prxs can be regulated by changes to phosphorylation, redox and possibly oligomerisation states. Prxs are divided into three classes: typical 2-Cys Prxs; atypical 2-Cys Prxs; and 1-Cys Prxs. All Prxs share the same basic catalytic mechanism, in which an active-site cysteine (the peroxidatic cysteine) is oxidised to a sulphenic acid by the peroxide substrate. The recycling of the sulphenic acid back to a thiol is what distinguishes the three enzyme classes. Using crystal structures, a detailed catalytic cycle has been derived for typical 2-Cys Prxs, including a model for the redox-regulated oligomeric state proposed to control enzyme activity [ PUBMED:12517450 ].

Alkyl hydroperoxide reductase (Ahp) has two subunits, the small AhpC subunit and the large AhpF subunit [ PUBMED:30657885 ]. AhpC is responsible for directly reducing organic hydroperoxides in its reduced dithiol form. Thiol specific antioxidant (TSA) is a physiologically important antioxidant which constitutes an enzymatic defence against sulphur-containing radicals and protects the cell against the oxidative stress caused by protein misfolding and aggregation [ PUBMED:24424024 ]. This entry contains AhpC and TSA, as well as related proteins.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(86)
Full
(46928)
Representative proteomes UniProt
(195026)
RP15
(6588)
RP35
(21967)
RP55
(46605)
RP75
(78126)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(86)
Full
(46928)
Representative proteomes UniProt
(195026)
RP15
(6588)
RP35
(21967)
RP55
(46605)
RP75
(78126)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(86)
Full
(46928)
Representative proteomes UniProt
(195026)
RP15
(6588)
RP35
(21967)
RP55
(46605)
RP75
(78126)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: MRC-LMB Genome group
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 86
Number in full: 46928
Average length of the domain: 126.70 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 56.41 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.6 21.6
Trusted cut-off 21.6 21.6
Noise cut-off 21.5 21.5
Model length: 124
Family (HMM) version: 23
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the AhpC-TSA domain has been found. There are 1037 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2K3Z9 View 3D Structure Click here
A0A0K3AUJ9 View 3D Structure Click here
A0A0P0WAE5 View 3D Structure Click here
A0A0R0HQQ2 View 3D Structure Click here
A0A0R0K508 View 3D Structure Click here
A0A0R0KTK4 View 3D Structure Click here
A0A0R4J2P7 View 3D Structure Click here
A0A1D6L317 View 3D Structure Click here
A0A2R8Q8M1 View 3D Structure Click here
A1Z892 View 3D Structure Click here
A1Z893 View 3D Structure Click here
A2AWE1 View 3D Structure Click here
A2SZW8 View 3D Structure Click here
A4HWK2 View 3D Structure Click here
A4HWK3 View 3D Structure Click here
A8E7G6 View 3D Structure Click here
B0G133 View 3D Structure Click here
B4FM07 View 3D Structure Click here
B4FS55 View 3D Structure Click here
B4FYU6 View 3D Structure Click here
B6U038 View 3D Structure Click here
C4J9M7 View 3D Structure Click here
F1QSP8 View 3D Structure Click here
I1JYW6 View 3D Structure Click here
I1KRJ7 View 3D Structure Click here
I1LY94 View 3D Structure Click here
I1MS47 View 3D Structure Click here
I6XYM2 View 3D Structure Click here
K7MM65 View 3D Structure Click here
K7MZB4 View 3D Structure Click here
O04005 View 3D Structure Click here
O08709 View 3D Structure Click here
O08807 View 3D Structure Click here
O35244 View 3D Structure Click here
O42666 View 3D Structure Click here
O53923 View 3D Structure Click here
O74887 View 3D Structure Click here
O94561 View 3D Structure Click here
P0A0B7 View 3D Structure Click here
P0AE08 View 3D Structure Click here
P0AE52 View 3D Structure Click here
P0C5C9 View 3D Structure Click here
P0C5D1 View 3D Structure Click here
P0C5D5 View 3D Structure Click here
P0CU34 View 3D Structure Click here
P20108 View 3D Structure Click here
P30041 View 3D Structure Click here
P30048 View 3D Structure Click here
P32119 View 3D Structure Click here
P34227 View 3D Structure Click here
P34760 View 3D Structure Click here
P35700 View 3D Structure Click here
P35704 View 3D Structure Click here
P40553 View 3D Structure Click here
P71990 View 3D Structure Click here
P9WG63 View 3D Structure Click here
P9WG65 View 3D Structure Click here
P9WID9 View 3D Structure Click here
P9WIE1 View 3D Structure Click here
P9WIE3 View 3D Structure Click here
P9WQB7 View 3D Structure Click here
Q04120 View 3D Structure Click here
Q06830 View 3D Structure Click here
Q13162 View 3D Structure Click here
Q21824 View 3D Structure Click here
Q2G280 View 3D Structure Click here
Q4CM56 View 3D Structure Click here
Q4CVR5 View 3D Structure Click here
Q4CX87 View 3D Structure Click here
Q4CY92 View 3D Structure Click here
Q4D2Z9 View 3D Structure Click here
Q4D301 View 3D Structure Click here
Q54NG4 View 3D Structure Click here
Q54SE2 View 3D Structure Click here
Q54W09 View 3D Structure Click here
Q54W30 View 3D Structure Click here
Q552Z0 View 3D Structure Click here
Q555L5 View 3D Structure Click here
Q559T9 View 3D Structure Click here
Q55E48 View 3D Structure Click here
Q55EE4 View 3D Structure Click here
Q55EF1 View 3D Structure Click here
Q55FA7 View 3D Structure Click here
Q58146 View 3D Structure Click here
Q5A5A0 View 3D Structure Click here
Q5A7P9 View 3D Structure Click here
Q61171 View 3D Structure Click here
Q63716 View 3D Structure Click here
Q6DGJ6 View 3D Structure Click here
Q6ER94 View 3D Structure Click here
Q7F229 View 3D Structure Click here
Q7JX87 View 3D Structure Click here
Q8BG37 View 3D Structure Click here
Q8I5Q6 View 3D Structure Click here
Q8IAM2 View 3D Structure Click here
Q8IJD0 View 3D Structure Click here
Q8IL80 View 3D Structure Click here
Q8MXT1 View 3D Structure Click here
Q95NF5 View 3D Structure Click here
Q95U89 View 3D Structure Click here
Q96291 View 3D Structure Click here
Q9C5R8 View 3D Structure Click here
Q9LU86 View 3D Structure Click here
Q9V3P0 View 3D Structure Click here
Q9V3Q4 View 3D Structure Click here
Q9VEJ0 View 3D Structure Click here
Q9VQI7 View 3D Structure Click here
Q9VUM4 View 3D Structure Click here
Q9Y7F0 View 3D Structure Click here
Q9Z0V5 View 3D Structure Click here
Q9Z0V6 View 3D Structure Click here