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111  structures 8337  species 0  interactions 27674  sequences 472  architectures

Family: Guanylate_kin (PF00625)

Summary: Guanylate kinase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Guanylate kinase". More...

Guanylate kinase Edit Wikipedia article

guanylate kinase
2an9.jpg
guanylate kinase homohexamer, E.Coli
Identifiers
EC number2.7.4.8
CAS number9026-59-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
guanylate kinase
PDB 1gky EBI.jpg
Structure of Guanylate Kinase.[1]
Identifiers
SymbolGuanylate_kin
PfamPF00625
InterProIPR008144
PROSITEPDOC00670
SCOPe1gky / SUPFAM
CDDcd00071

In enzymology, a guanylate kinase (EC 2.7.4.8) is an enzyme that catalyzes the chemical reaction

ATP + GMP ADP + GDP

Thus, the two substrates of this enzyme are ATP and GMP, whereas its two products are ADP and GDP.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. This enzyme participates in purine metabolism.

Guanylate kinase catalyzes the ATP-dependent phosphorylation of GMP into GDP.[1] It is essential for recycling GMP and indirectly, cGMP. In prokaryotes (such as Escherichia coli), lower eukaryotes (such as yeast) and in vertebrates, GK is a highly conserved monomeric protein of about 200 amino acids. GK has been shown to be structurally similar to protein A57R (or SalG2R) from various strains of Vaccinia virus.[2][3][4]

Nomenclature

The systematic name of this enzyme class is ATP:(d)GMP phosphotransferase. Other names in common use include"

  • deoxyguanylate kinase,
  • 5'-GMP kinase,
  • GMP kinase,
  • guanosine monophosphate kinase, and
  • ATP:GMP phosphotransferase.

References

  1. ^ a b Stehle T, Schulz GE (April 1992). "Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution". J. Mol. Biol. 224 (4): 1127–41. doi:10.1016/0022-2836(92)90474-X. PMID 1314905.
  2. ^ Bryant PJ, Woods DF (February 1992). "A major palmitoylated membrane protein of human erythrocytes shows homology to yeast guanylate kinase and to the product of a Drosophila tumor suppressor gene". Cell. 68 (4): 621–2. doi:10.1016/0092-8674(92)90136-Z. PMID 1310897.
  3. ^ Zschocke PD, Schiltz E, Schulz GE (April 1993). "Purification and sequence determination of guanylate kinase from pig brain". Eur. J. Biochem. 213 (1): 263–9. doi:10.1111/j.1432-1033.1993.tb17757.x. PMID 8097461.
  4. ^ Goebl MG (March 1992). "Is the erythrocyte protein p55 a membrane-bound guanylate kinase?". Trends Biochem. Sci. 17 (3): 99. doi:10.1016/0968-0004(92)90244-4. PMID 1329277.

Further reading

  • Buccino RJ Jr, Roth JS (1969). "Partial purification and properties of ATP:GMP phosphotransferase from rat liver". Arch. Biochem. Biophys. 132 (1): 49–61. doi:10.1016/0003-9861(69)90337-3. PMID 4307347.
  • Hiraga S, Sugino Y (1966). "Nucleoside monophosphokinases of Escherichia coli infected and uninfected with an RNA phage". Biochim. Biophys. Acta. 114 (2): 416–8. doi:10.1016/0005-2787(66)90324-8. PMID 5329274.
  • Griffith TJ, Helleiner CW (1965). "The partial purification of deoxynucleoside monophosphate kinases from L cells". Biochim. Biophys. Acta. 108 (1): 114–24. doi:10.1016/0005-2787(65)90113-9. PMID 5862227.
  • Oeschger MP, Bessman MJ (1966). "Purification and properties of guanylate kinase from Escherichia coli". J. Biol. Chem. 241 (22): 5452–60. PMID 5333666.
  • Shimono H, Sugino Y (1971). "Metabolism of deoxyribonucleotides. Purification and properties of deoxyguanosine monophosphokinase of calf thymus". Eur. J. Biochem. 19 (2): 256–63. doi:10.1111/j.1432-1033.1971.tb01312.x. PMID 5552394.
This article incorporates text from the public domain Pfam and InterPro: IPR008144


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

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No Pfam abstract.

Literature references

  1. Stehle T, Schulz GE; , J Mol Biol 1992;224:1127-1141.: Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution. PUBMED:1314905 EPMC:1314905


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR008145

This entry represents a domain found in guanylate kinase ( EC ) and in L-type calcium channel.

Guanylate kinase ( EC ) (GK) [ PUBMED:1314905 ] catalyzes the ATP-dependent phosphorylation of GMP into GDP. It is essential for recycling GMP and indirectly, cGMP. In prokaryotes (such as Escherichia coli), lower eukaryotes (such as yeast) and in vertebrates, GK is a highly conserved monomeric protein of about 200 amino acids. GK has been shown [ PUBMED:1310897 , PUBMED:8097461 , PUBMED:1329277 ] to be structurally similar to protein A57R (or SalG2R) from various strains of Vaccinia virus.

L-type calcium channnels are formed from different alpha-1 subunit isoforms that determine the pharmacological properties of the channel, since they form the drug binding domain. Other properties, such as gating voltage-dependence, G protein modulation and kinase susceptibility, are influenced by alpha-2, delta and beta subunits.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan P-loop_NTPase (CL0023), which has the following description:

AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes [2].

The clan contains the following 243 members:

6PF2K AAA AAA-ATPase_like AAA_10 AAA_11 AAA_12 AAA_13 AAA_14 AAA_15 AAA_16 AAA_17 AAA_18 AAA_19 AAA_2 AAA_21 AAA_22 AAA_23 AAA_24 AAA_25 AAA_26 AAA_27 AAA_28 AAA_29 AAA_3 AAA_30 AAA_31 AAA_32 AAA_33 AAA_34 AAA_35 AAA_5 AAA_6 AAA_7 AAA_8 AAA_9 AAA_PrkA ABC_ATPase ABC_tran ABC_tran_Xtn Adeno_IVa2 Adenylsucc_synt ADK AFG1_ATPase AIG1 APS_kinase Arf ArsA_ATPase ATP-synt_ab ATP_bind_1 ATP_bind_2 ATPase ATPase_2 Bac_DnaA BCA_ABC_TP_C Beta-Casp bpMoxR Cas_Csn2 Cas_St_Csn2 CbiA CBP_BcsQ CDC73_C CENP-M CFTR_R CLP1_P CMS1 CoaE CobA_CobO_BtuR CobU cobW CPT CSM2 CTP_synth_N Cytidylate_kin Cytidylate_kin2 DAP3 DBINO DEAD DEAD_2 DLIC DNA_pack_C DNA_pack_N DNA_pol3_delta DNA_pol3_delta2 DnaB_C dNK DO-GTPase1 DO-GTPase2 DUF1611 DUF1726 DUF2075 DUF2326 DUF2478 DUF257 DUF2791 DUF2813 DUF3584 DUF463 DUF5906 DUF6079 DUF815 DUF853 DUF87 DUF927 Dynamin_N Dynein_heavy Elong_Iki1 ELP6 ERCC3_RAD25_C Exonuc_V_gamma FeoB_N Fer4_NifH Flavi_DEAD FTHFS FtsK_SpoIIIE G-alpha Gal-3-0_sulfotr GBP GBP_C GTP_EFTU Gtr1_RagA Guanylate_kin GvpD HDA2-3 Helicase_C Helicase_C_2 Helicase_C_4 Helicase_RecD Herpes_Helicase Herpes_ori_bp Herpes_TK HSA HydF_dimer HydF_tetramer Hydin_ADK IIGP IPPT IPT iSTAND IstB_IS21 KAP_NTPase KdpD Kinase-PPPase Kinesin KTI12 LAP1C Lon_2 LpxK MCM MeaB MEDS Mg_chelatase Microtub_bd MipZ MMR_HSR1 MMR_HSR1_C MobB MukB Mur_ligase_M MutS_V Myosin_head NACHT NAT_N NB-ARC NOG1 NTPase_1 NTPase_P4 OPA1_C ORC3_N P-loop_TraG ParA Parvo_NS1 PAXNEB PduV-EutP PhoH PIF1 Ploopntkinase1 Ploopntkinase2 Ploopntkinase3 Podovirus_Gp16 Polyoma_lg_T_C Pox_A32 PPK2 PPV_E1_C PRK PSY3 Rad17 Rad51 Ras RecA ResIII RHD3 RhoGAP_pG1_pG2 RHSP RNA12 RNA_helicase Roc RsgA_GTPase RuvB_N SbcCD_C SecA_DEAD Septin Sigma54_activ_2 Sigma54_activat SKI SMC_N SNF2-rel_dom Spore_III_AA Spore_IV_A SRP54 SRPRB SulA Sulfotransfer_1 Sulfotransfer_2 Sulfotransfer_3 Sulfotransfer_4 Sulfotransfer_5 Sulphotransf SWI2_SNF2 T2SSE T4SS-DNA_transf Terminase_1 Terminase_3 Terminase_6N Terminase_GpA Thymidylate_kin TIP49 TK TniB Torsin TraG-D_C tRNA_lig_kinase TrwB_AAD_bind TsaE UvrB UvrD-helicase UvrD_C UvrD_C_2 Viral_helicase1 VirC1 VirE YqeC Zeta_toxin Zot

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(11)
Full
(27674)
Representative proteomes UniProt
(75097)
RP15
(3377)
RP35
(10785)
RP55
(25254)
RP75
(38067)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(11)
Full
(27674)
Representative proteomes UniProt
(75097)
RP15
(3377)
RP35
(10785)
RP55
(25254)
RP75
(38067)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(11)
Full
(27674)
Representative proteomes UniProt
(75097)
RP15
(3377)
RP35
(10785)
RP55
(25254)
RP75
(38067)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Bateman A
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 11
Number in full: 27674
Average length of the domain: 164.40 aa
Average identity of full alignment: 26 %
Average coverage of the sequence by the domain: 27.60 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.2 25.2
Trusted cut-off 25.2 25.2
Noise cut-off 25.1 25.1
Model length: 182
Family (HMM) version: 23
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
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Viroids Viroids Unclassified sequence Unclassified sequence

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Guanylate_kin domain has been found. There are 111 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0B4K6Y7 View 3D Structure Click here
A0A0G2JWS1 View 3D Structure Click here
A0A0G2K2P5 View 3D Structure Click here
A0A0G2K4N7 View 3D Structure Click here
A0A0H2UKX0 View 3D Structure Click here
A0A0R0ELX3 View 3D Structure Click here
A0A0R0L847 View 3D Structure Click here
A0A0R4IFE8 View 3D Structure Click here
A0A0R4IGT8 View 3D Structure Click here
A0A1D5NS76 View 3D Structure Click here
A0A1D6K135 View 3D Structure Click here
A0A1D8PNS0 View 3D Structure Click here
A0A286Y9N6 View 3D Structure Click here
A0A286YB86 View 3D Structure Click here
A0A2R8Q552 View 3D Structure Click here
A0A2R8QB05 View 3D Structure Click here
A0A2R8QE05 View 3D Structure Click here
A0A2R8QET5 View 3D Structure Click here
A0A2R8QMK9 View 3D Structure Click here
A0A2R8QSV4 View 3D Structure Click here
A0A2R8RHW5 View 3D Structure Click here
A0A2R8RJH6 View 3D Structure Click here
A0A2R8RTY0 View 3D Structure Click here
A0A2R8S0F4 View 3D Structure Click here
A0A3B1DQX2 View 3D Structure Click here
A1Z8G0 View 3D Structure Click here
A2SZ53 View 3D Structure Click here
A4I8X4 View 3D Structure Click here
A4IDK0 View 3D Structure Click here
A4IDK0 View 3D Structure Click here
A5WV25 View 3D Structure Click here
B0F0D0 View 3D Structure Click here
B0F0D1 View 3D Structure Click here
B0UXH8 View 3D Structure Click here
B3DH78 View 3D Structure Click here
B4F7E7 View 3D Structure Click here
B4FV93 View 3D Structure Click here
B8JJJ5 View 3D Structure Click here
B8JJJ5 View 3D Structure Click here
C1P633 View 3D Structure Click here
D3Z8G7 View 3D Structure Click here
D3ZAA9 View 3D Structure Click here
D4A055 View 3D Structure Click here
D4A3K3 View 3D Structure Click here
D4A8M2 View 3D Structure Click here
E2QD98 View 3D Structure Click here
E7EXQ0 View 3D Structure Click here
E7EZI7 View 3D Structure Click here
E7F210 View 3D Structure Click here
E7F7X1 View 3D Structure Click here
E7FH65 View 3D Structure Click here
E9PTV0 View 3D Structure Click here
E9Q9R9 View 3D Structure Click here
E9QIL2 View 3D Structure Click here
F1Q897 View 3D Structure Click here
F1Q9P1 View 3D Structure Click here
F1QCS0 View 3D Structure Click here
F1QKE3 View 3D Structure Click here
F1QS98 View 3D Structure Click here
F1QV76 View 3D Structure Click here
F1R2J9 View 3D Structure Click here
F1R3X0 View 3D Structure Click here
G5ECY0 View 3D Structure Click here
I1JNE4 View 3D Structure Click here
I1LAB8 View 3D Structure Click here
I1MFD0 View 3D Structure Click here
K7M0X6 View 3D Structure Click here
M9PD84 View 3D Structure Click here
O00305 View 3D Structure Click here
O01661 View 3D Structure Click here
O14936 View 3D Structure Click here
O70589 View 3D Structure Click here
O88382 View 3D Structure Click here
O88910 View 3D Structure Click here
O88954 View 3D Structure Click here
O95049 View 3D Structure Click here
P15454 View 3D Structure Click here
P31007 View 3D Structure Click here
P31016 View 3D Structure Click here
P39447 View 3D Structure Click here
P54283 View 3D Structure Click here
P54284 View 3D Structure Click here
P54285 View 3D Structure Click here
P54287 View 3D Structure Click here
P54936 View 3D Structure Click here
P60546 View 3D Structure Click here
P70175 View 3D Structure Click here
P70290 View 3D Structure Click here
P78352 View 3D Structure Click here
P91182 View 3D Structure Click here
P91425 View 3D Structure Click here
P93757 View 3D Structure Click here
P9WKE9 View 3D Structure Click here
Q00013 View 3D Structure Click here
Q02641 View 3D Structure Click here
Q07157 View 3D Structure Click here
Q08289 View 3D Structure Click here
Q10M74 View 3D Structure Click here
Q12959 View 3D Structure Click here
Q13368 View 3D Structure Click here
Q14168 View 3D Structure Click here
Q15700 View 3D Structure Click here
Q16774 View 3D Structure Click here
Q1L8G4 View 3D Structure Click here
Q1LW94 View 3D Structure Click here
Q24210 View 3D Structure Click here
Q2G1U0 View 3D Structure Click here
Q2Q551 View 3D Structure Click here
Q2QPW1 View 3D Structure Click here
Q3ZB99 View 3D Structure Click here
Q4DPB1 View 3D Structure Click here
Q4E4D6 View 3D Structure Click here
Q4E4D6 View 3D Structure Click here
Q4L1J4 View 3D Structure Click here
Q54XF2 View 3D Structure Click here
Q5PYH5 View 3D Structure Click here
Q5PYH6 View 3D Structure Click here
Q5PYH7 View 3D Structure Click here
Q5T2T1 View 3D Structure Click here
Q5TCQ9 View 3D Structure Click here
Q5U2Y3 View 3D Structure Click here
Q62108 View 3D Structure Click here
Q62696 View 3D Structure Click here
Q62915 View 3D Structure Click here
Q62936 View 3D Structure Click here
Q63622 View 3D Structure Click here
Q64520 View 3D Structure Click here
Q6P0D7 View 3D Structure Click here
Q6P7F1 View 3D Structure Click here
Q6R005 View 3D Structure Click here
Q6RHR9 View 3D Structure Click here
Q7KT16 View 3D Structure Click here
Q811D0 View 3D Structure Click here
Q86UL8 View 3D Structure Click here
Q8BVD5 View 3D Structure Click here
Q8CC27 View 3D Structure Click here
Q8I103 View 3D Structure Click here
Q8I2M1 View 3D Structure Click here
Q8JHF4 View 3D Structure Click here
Q8N3R9 View 3D Structure Click here
Q8R0S4 View 3D Structure Click here
Q8R3Z5 View 3D Structure Click here
Q8TDM6 View 3D Structure Click here
Q8VGC3 View 3D Structure Click here
Q91XM9 View 3D Structure Click here
Q92796 View 3D Structure Click here
Q94JM2 View 3D Structure Click here
Q95XW5 View 3D Structure Click here
Q96JB8 View 3D Structure Click here
Q96M69 View 3D Structure Click here
Q96QZ7 View 3D Structure Click here
Q9D5S7 View 3D Structure Click here
Q9EQJ9 View 3D Structure Click here
Q9JK71 View 3D Structure Click here
Q9JLB0 View 3D Structure Click here
Q9JLB2 View 3D Structure Click here
Q9M682 View 3D Structure Click here
Q9NZW5 View 3D Structure Click here
Q9P6I5 View 3D Structure Click here
Q9QXY1 View 3D Structure Click here
Q9QYH1 View 3D Structure Click here
Q9UDY2 View 3D Structure Click here
Q9VKG8 View 3D Structure Click here
Q9VTB3 View 3D Structure Click here
Q9WV34 View 3D Structure Click here
Q9WVQ1 View 3D Structure Click here
Q9Z0U1 View 3D Structure Click here
X1WFJ8 View 3D Structure Click here