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290  structures 8212  species 0  interactions 42197  sequences 304  architectures

Family: CN_hydrolase (PF00795)

Summary: Carbon-nitrogen hydrolase

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Carbon-nitrogen hydrolase Provide feedback

This family contains hydrolases that break carbon-nitrogen bonds [1]. The family includes: Nitrilase EC:3.5.5.1 Q42965 Aliphatic amidase EC:3.5.1.4 Q01360 Biotidinase EC:3.5.1.12 P43251 Beta-ureidopropionase EC:3.5.1.6 Q03248. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad, and are multimeric alpha-beta-beta-alpha sandwich proteins [2].

Literature references

  1. Bork P, Koonin EV; , Protein Sci 1994;3:1344-1346.: A new family of carbon-nitrogen hydrolases. PUBMED:7987228 EPMC:7987228

  2. Pace HC, Brenner C; , Genome Biol 2001;2:REVIEWS0001.: The nitrilase superfamily: classification, structure and function. PUBMED:11380987 EPMC:11380987


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR003010

The carbon-nitrogen hydrolase domain is an around 265-residue domain found in numerous enzymes involved in the reduction of organic nitrogen compounds and ammonia production. Based on their sequence similarity and on the reactions they catalyse, these enzymes can be classified into functionally distinct groups including [ PUBMED:7987228 , PUBMED:12054553 ]:

  • Nitrilases ( EC ), which cleave various nitriles into the corresponding acids and ammonia.
  • Cyanide hydratase ( EC ) of pathogenic fungi, which detoxifies HCN that is released by their hosts, cyanogenic plants, after injury.
  • Aliphatic amidases ( EC ), which enable prokaryotes to use acetamides as both carbon and nitrogen source.
  • Beta-ureidopropionase (also known as beta-alanine synthase or N-carbamoyl-beta-alanine amino hydrolase; EC ), which catalyses the last step of pyrimidine catabolism.
  • Glutamine-dependent NAD(+) synthetase (also known as AdgA, for ammonia-dependent growth) from Rhodobacter species ( EC ). It appears to be essential for using various amino acids as nitrogen sources.
  • Biotinidase ( EC ), which catalyses the hydrolysis of biocytin to biotin and lysine.
  • Pantetheinase ( EC ) (Pantetheine hydrolase) (Vanin), which hydrolyzes specifically one of the carboamide linkages in D-pantetheine, thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
  • Apolipoprotein N-acyltransferase ([intenz:2.3.1.-]) (gene lnt), a bacterial enzyme that transfers the fatty acyl group on membrane lipoproteins.
  • Glutamine-dependent NAD(+) synthetase ( EC ), which catalyses the final step in NAD+ synthesis [ PUBMED:12771147 , PUBMED:12898714 ].

The carbon-nitrogen hydrolase domain is characterised by several conserved motifs, one of which contains a cysteine that is part of the catalytic site in nitrilases. Another highly conserved motif includes a glutamic acid that might also be involved in catalysis [ PUBMED:7987228 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(52)
Full
(42197)
Representative proteomes UniProt
(171227)
RP15
(5611)
RP35
(19069)
RP55
(39957)
RP75
(67919)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(52)
Full
(42197)
Representative proteomes UniProt
(171227)
RP15
(5611)
RP35
(19069)
RP55
(39957)
RP75
(67919)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(52)
Full
(42197)
Representative proteomes UniProt
(171227)
RP15
(5611)
RP35
(19069)
RP55
(39957)
RP75
(67919)
Raw Stockholm Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1042 (release 2.1) & Pfam-B_5155 (Release 7.5)
Previous IDs: Nitrilase;
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A , Eberhardt R
Number in seed: 52
Number in full: 42197
Average length of the domain: 250.10 aa
Average identity of full alignment: 19 %
Average coverage of the sequence by the domain: 62.50 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.2 23.2
Trusted cut-off 23.2 23.2
Noise cut-off 23.1 23.1
Model length: 261
Family (HMM) version: 24
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CN_hydrolase domain has been found. There are 290 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0WU26 View 3D Structure Click here
A0A0R0E5N3 View 3D Structure Click here
A0A0R0HW00 View 3D Structure Click here
A0A0R4ID24 View 3D Structure Click here
A0A0R4IPE7 View 3D Structure Click here
A0A1D6EKN6 View 3D Structure Click here
A0A1D6HE88 View 3D Structure Click here
A0A1D6IV77 View 3D Structure Click here
A0A1D6JQB2 View 3D Structure Click here
A0A1D6PJ22 View 3D Structure Click here
A0A1D8PGW9 View 3D Structure Click here
A0A1D8PTV6 View 3D Structure Click here
A0A2R8QJP7 View 3D Structure Click here
A4I2F5 View 3D Structure Click here
B0V1I3 View 3D Structure Click here
B4FQE2 View 3D Structure Click here
B4FSZ8 View 3D Structure Click here
B6TKZ3 View 3D Structure Click here
C6T972 View 3D Structure Click here
C6TN61 View 3D Structure Click here
D4A183 View 3D Structure Click here
I1K669 View 3D Structure Click here
I1K8F4 View 3D Structure Click here
I1KL55 View 3D Structure Click here
I1KPN4 View 3D Structure Click here
I1LFG0 View 3D Structure Click here
I1LTW8 View 3D Structure Click here
I1M4B9 View 3D Structure Click here
I6XDW5 View 3D Structure Click here
K7N5E3 View 3D Structure Click here
O53493 View 3D Structure Click here
O53536 View 3D Structure Click here
O59829 View 3D Structure Click here
O60178 View 3D Structure Click here
O74940 View 3D Structure Click here
O76463 View 3D Structure Click here
O76464 View 3D Structure Click here
O94660 View 3D Structure Click here
O95497 View 3D Structure Click here
O95498 View 3D Structure Click here
P0DP64 View 3D Structure Click here
P23930 View 3D Structure Click here
P32961 View 3D Structure Click here
P32962 View 3D Structure Click here
P38795 View 3D Structure Click here
P40354 View 3D Structure Click here
P40446 View 3D Structure Click here
P40447 View 3D Structure Click here
P43251 View 3D Structure Click here
P46010 View 3D Structure Click here
P46011 View 3D Structure Click here
P47016 View 3D Structure Click here
P49954 View 3D Structure Click here
P76280 View 3D Structure Click here
P83548 View 3D Structure Click here
P9WJ01 View 3D Structure Click here
P9WJJ3 View 3D Structure Click here
Q03248 View 3D Structure Click here
Q0D8D4 View 3D Structure Click here
Q10166 View 3D Structure Click here
Q10R10 View 3D Structure Click here
Q19437 View 3D Structure Click here
Q23384 View 3D Structure Click here
Q2FWM9 View 3D Structure Click here
Q2QQ94 View 3D Structure Click here
Q47679 View 3D Structure Click here
Q497B0 View 3D Structure Click here
Q4D9K8 View 3D Structure Click here
Q4E2D4 View 3D Structure Click here
Q4KLZ0 View 3D Structure Click here
Q4VBV9 View 3D Structure Click here
Q54JM9 View 3D Structure Click here
Q54ML1 View 3D Structure Click here
Q54WG1 View 3D Structure Click here
Q557J5 View 3D Structure Click here
Q59WF0 View 3D Structure Click here
Q5ALW6 View 3D Structure Click here
Q5FVF9 View 3D Structure Click here
Q6H849 View 3D Structure Click here
Q6H851 View 3D Structure Click here
Q6IA69 View 3D Structure Click here
Q711T7 View 3D Structure Click here
Q7TQ94 View 3D Structure Click here
Q812E8 View 3D Structure Click here
Q86X76 View 3D Structure Click here
Q8CIF4 View 3D Structure Click here
Q8H183 View 3D Structure Click here
Q8H530 View 3D Structure Click here
Q8IRR1 View 3D Structure Click here
Q8RUF8 View 3D Structure Click here
Q8VC97 View 3D Structure Click here
Q8VDK1 View 3D Structure Click here
Q8VYF5 View 3D Structure Click here
Q93XI4 View 3D Structure Click here
Q94JV5 View 3D Structure Click here
Q964D8 View 3D Structure Click here
Q9C723 View 3D Structure Click here
Q9JHW2 View 3D Structure Click here
Q9NFP1 View 3D Structure Click here
Q9NQR4 View 3D Structure Click here
Q9NY84 View 3D Structure Click here
Q9QZ25 View 3D Structure Click here
Q9UBR1 View 3D Structure Click here
Q9VHE4 View 3D Structure Click here
Q9VI04 View 3D Structure Click here
Q9VYA0 View 3D Structure Click here
Q9W430 View 3D Structure Click here
Q9XXK6 View 3D Structure Click here
Q9Z0K8 View 3D Structure Click here