Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
225  structures 4217  species 0  interactions 18081  sequences 518  architectures

Family: Glyco_hydro_31 (PF01055)

Summary: Glycosyl hydrolases family 31

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Glycoside hydrolase family 31". More...

Glycoside hydrolase family 31 Edit Wikipedia article

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Glycosyl hydrolases family 31 Provide feedback

Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.

Literature references

  1. Henrissat B; , Biochem J 1991;280:309-316.: A classification of glycosyl hydrolases based on amino acid sequence similarities. PUBMED:1747104 EPMC:1747104

  2. Henrissat B , Biochem Soc Trans 1998;26:153-156.: Glycosidase families PUBMED:9649738 EPMC:9649738


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000322

O-Glycosyl hydrolases ( EC ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ PUBMED:7624375 , PUBMED:8535779 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website.

Glycoside hydrolase family 31 CAZY comprises enzymes with several known activities; alpha-glucosidase ( EC ), alpha-galactosidase ( EC ); glucoamylase ( EC ), sucrase-isomaltase ( EC ); isomaltase ( EC ); alpha-xylosidase ( EC ); alpha-glucan lyase ( EC ).

Glycoside hydrolase family 31 groups a number of glycosyl hydrolases on the basis of sequence similarities [ PUBMED:1747104 , PUBMED:1761061 , PUBMED:1743281 ]. An aspartic acid has been implicated [ PUBMED:1856189 ] in the catalytic activity of sucrase, isomaltase, and lysosomal alpha-glucosidase.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(663)
Full
(18081)
Representative proteomes UniProt
(51817)
RP15
(2895)
RP35
(8231)
RP55
(15865)
RP75
(23828)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(663)
Full
(18081)
Representative proteomes UniProt
(51817)
RP15
(2895)
RP35
(8231)
RP55
(15865)
RP75
(23828)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(663)
Full
(18081)
Representative proteomes UniProt
(51817)
RP15
(2895)
RP35
(8231)
RP55
(15865)
RP75
(23828)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_369 (release 3.0)
Previous IDs: Glycosyl_hydr15;
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD , Bateman A
Number in seed: 663
Number in full: 18081
Average length of the domain: 405.30 aa
Average identity of full alignment: 26 %
Average coverage of the sequence by the domain: 52.73 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null --hand HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 32.0 32.0
Trusted cut-off 32.0 32.0
Noise cut-off 31.9 31.9
Model length: 444
Family (HMM) version: 28
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_31 domain has been found. There are 225 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2KY72 View 3D Structure Click here
A0A0G2L1N0 View 3D Structure Click here
A0A0G2L1N0 View 3D Structure Click here
A0A0P0X5N3 View 3D Structure Click here
A0A0R0G0B7 View 3D Structure Click here
A0A0R0G0B7 View 3D Structure Click here
A0A0R0I6Y6 View 3D Structure Click here
A0A0R0JT00 View 3D Structure Click here
A0A1D6EMY1 View 3D Structure Click here
A0A1D6FBD3 View 3D Structure Click here
A0A1D6HXT2 View 3D Structure Click here
A0A1D6LPQ2 View 3D Structure Click here
A0A1D6MM31 View 3D Structure Click here
A0A1I9LTS6 View 3D Structure Click here
A0A2R8QI15 View 3D Structure Click here
A0A2R8QK09 View 3D Structure Click here
A0A571BDS5 View 3D Structure Click here
A0A571BDS5 View 3D Structure Click here
A4HXN5 View 3D Structure Click here
A4IE64 View 3D Structure Click here
B3DIZ3 View 3D Structure Click here
B9F676 View 3D Structure Click here
C0PHF3 View 3D Structure Click here
D3ZAN3 View 3D Structure Click here
D3ZTX4 View 3D Structure Click here
D3ZTX4 View 3D Structure Click here
D4A7G5 View 3D Structure Click here
D4AE63 View 3D Structure Click here
E7F1U0 View 3D Structure Click here
E7F6D4 View 3D Structure Click here
E7FCP7 View 3D Structure Click here
E7FGC0 View 3D Structure Click here
F1Q6Z9 View 3D Structure Click here
F1Q8T6 View 3D Structure Click here
F4J6T7 View 3D Structure Click here
F8VQM5 View 3D Structure Click here
F8VQM5 View 3D Structure Click here
I1J6K4 View 3D Structure Click here
I1JVV8 View 3D Structure Click here
I1JXY2 View 3D Structure Click here
I1KWV4 View 3D Structure Click here
I1LEX2 View 3D Structure Click here
I1MG74 View 3D Structure Click here
K7KEN9 View 3D Structure Click here
K7MB60 View 3D Structure Click here
K7VP34 View 3D Structure Click here
O17352 View 3D Structure Click here
O43451 View 3D Structure Click here
O43451 View 3D Structure Click here
O74254 View 3D Structure Click here
P10253 View 3D Structure Click here
P14410 View 3D Structure Click here
P14410 View 3D Structure Click here
P23739 View 3D Structure Click here
P23739 View 3D Structure Click here
P31434 View 3D Structure Click here
P32138 View 3D Structure Click here
P38138 View 3D Structure Click here
P70699 View 3D Structure Click here
Q09901 View 3D Structure Click here
Q0D6X6 View 3D Structure Click here
Q0JQZ2 View 3D Structure Click here
Q14697 View 3D Structure Click here
Q19004 View 3D Structure Click here
Q20239 View 3D Structure Click here
Q21750 View 3D Structure Click here
Q2M2H8 View 3D Structure Click here
Q2M2H8 View 3D Structure Click here
Q4CZY1 View 3D Structure Click here
Q4DDQ4 View 3D Structure Click here
Q4DLH7 View 3D Structure Click here
Q4E1V5 View 3D Structure Click here
Q55D50 View 3D Structure Click here
Q5A4X3 View 3D Structure Click here
Q5NBJ1 View 3D Structure Click here
Q653V4 View 3D Structure Click here
Q653V7 View 3D Structure Click here
Q69ZQ1 View 3D Structure Click here
Q6NSJ0 View 3D Structure Click here
Q6P7A9 View 3D Structure Click here
Q7KMM4 View 3D Structure Click here
Q8BHN3 View 3D Structure Click here
Q8BVW0 View 3D Structure Click here
Q8TET4 View 3D Structure Click here
Q94502 View 3D Structure Click here
Q9C0Y4 View 3D Structure Click here
Q9FN05 View 3D Structure Click here
Q9LYF8 View 3D Structure Click here
Q9S7Y7 View 3D Structure Click here
Q9URX4 View 3D Structure Click here
Q9US55 View 3D Structure Click here
Q9VBR6 View 3D Structure Click here
Q9W490 View 3D Structure Click here
Q9W490 View 3D Structure Click here