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15  structures 1571  species 0  interactions 11261  sequences 180  architectures

Family: EMP24_GP25L (PF01105)

Summary: emp24/gp25L/p24 family/GOLD

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

emp24/gp25L/p24 family/GOLD Provide feedback

Members of this family are implicated in bringing cargo forward from the ER and binding to coat proteins by their cytoplasmic domains. This domain corresponds closely to the beta-strand rich GOLD domain described in [2]. The GOLD domain is always found combined with lipid- or membrane-association domains [2].

Literature references

  1. Dominguez M, Dejgaard K, Fullekrug J, Dahan S, Fazel A, Paccaud JP, Thomas DY, Bergeron JJ, Nilsson T; , J Cell Biol 1998;140:751-765.: gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer. PUBMED:9472029 EPMC:9472029

  2. Anantharaman V, Aravind L; , Genome Biol 2002;3:research0023.: The GOLD domain, a novel protein module involved in Golgi function and secretion. PUBMED:12049664 EPMC:12049664

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR009038

The GOLD (for Golgi dynamics) domain is a protein module found in several eukaryotic Golgi and lipid-traffic proteins. It is typically between 90 and 150 amino acids long. Most of the size difference observed in the GOLD-domain superfamily is traceable to a single large low-complexity insert that is seen in some versions of the domain. With the exception of the p24 proteins, which have a simple architecture with the GOLD domain as their only globular domain, all other GOLD-domain proteins contain additional conserved globular domains. In these proteins, the GOLD domain co-occurs with lipid-, sterol- or fatty acid-binding domains such as PH, CRAL-TRIO, FYVE oxysterol binding- and acyl CoA-binding domains, suggesting that these proteins may interact with membranes. The GOLD domain can also be found associated with a RUN domain, which may have a role in the interaction of various proteins with cytoskeletal filaments. The GOLD domain is predicted to mediate diverse protein-protein interactions [ PUBMED:12049664 ]. A secondary structure prediction for the GOLD domain reveals that it is likely to adopt a compact all-beta-fold structure with six to seven strands. Most of the sequence conservation is centred on the hydrophobic cores that support these predicted strands. The predicted secondary-structure elements and the size of the conserved core of the domain suggests that it may form a beta- sandwich fold with the strands arranged in two beta sheets stacked on each other [ PUBMED:12049664 ].

Some proteins known to contain a GOLD domain are listed below:

  • Eukaryotic proteins of the p24 family.
  • Animal Sec14-like proteins. They are involved in secretion.
  • Human Golgi resident protein GCP60. It interacts with the Golgi integral membrane protein Giantin.
  • Yeast oxysterol-binding protein homologue 3 (OSH3).

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan GOLD-like (CL0521), which has the following description:

This superfamily is characterised by proteins with a sandwich structure of 8 strands in 2 sheets as a jelly-roll. The structure has similarity to the Nucleoplasmin-like/VP fold. The members are all involved in golgi trafficking.

The clan contains the following 2 members:



We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

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Representative proteomes UniProt

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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_803 (release 3.0)
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Finn RD , Bateman A , Wuster A
Number in seed: 208
Number in full: 11261
Average length of the domain: 174.60 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 75.06 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.9 25.9
Trusted cut-off 25.9 25.9
Noise cut-off 25.8 25.7
Model length: 182
Family (HMM) version: 27
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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The tree shows the occurrence of this domain across different species. More...


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the EMP24_GP25L domain has been found. There are 15 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0VXR4 View 3D Structure Click here
A0A0P0XGL8 View 3D Structure Click here
A0A0R0F5W1 View 3D Structure Click here
A0A0R0GH40 View 3D Structure Click here
A0A0R0IL27 View 3D Structure Click here
A0A0R4IWV5 View 3D Structure Click here
A0A1D6ERA7 View 3D Structure Click here
A0A1D6HLC4 View 3D Structure Click here
A0A1D6P7G6 View 3D Structure Click here
A0A1D8PF48 View 3D Structure Click here
A0A368UIH1 View 3D Structure Click here
A0A5K1K8K6 View 3D Structure Click here
A0AQ71 View 3D Structure Click here
A3C0G9 View 3D Structure Click here
A4F319 View 3D Structure Click here
A4HW85 View 3D Structure Click here
A4I6N5 View 3D Structure Click here
A4I6N6 View 3D Structure Click here
A4I778 View 3D Structure Click here
A4I824 View 3D Structure Click here
A4I9J2 View 3D Structure Click here
A4IB85 View 3D Structure Click here
A4IDI9 View 3D Structure Click here
A4IDX1 View 3D Structure Click here
A7SXK3 View 3D Structure Click here
B3DKM3 View 3D Structure Click here
B3LVB0 View 3D Structure Click here
B3MTS8 View 3D Structure Click here
B4F881 View 3D Structure Click here
B4FE73 View 3D Structure Click here
B4FIW7 View 3D Structure Click here
B4FSU1 View 3D Structure Click here
B4FV79 View 3D Structure Click here
B4FVH9 View 3D Structure Click here
B4GE47 View 3D Structure Click here
B4GMC3 View 3D Structure Click here
B4HJV5 View 3D Structure Click here
B4JG34 View 3D Structure Click here
B4JYU5 View 3D Structure Click here
B4K4G5 View 3D Structure Click here