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0  structures 1218  species 0  interactions 2308  sequences 49  architectures

# Summary: Cytochrome c/c1 heme lyase

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This is the Wikipedia entry entitled "Holocytochrome-c synthase". More...

# Holocytochrome-c synthase

holocytochrome-c synthase
Identifiers
EC number4.4.1.17
CAS number75139-03-6
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structures
Gene Ontology
Cytochrome c/c1 heme lyase
Identifiers
SymbolCyto_heme_lyase
PfamPF01265
InterProIPR000511
PROSITEPDOC00647

In enzymology, a holocytochrome-c synthase (EC 4.4.1.17) is an enzyme that catalyzes the chemical reaction

holocytochrome c ${\displaystyle \rightleftharpoons }$ apocytochrome c + heme

Hence, this enzyme has one substrate, holocytochrome c, and two products, apocytochrome c and heme.

This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The systematic name of this enzyme class is holocytochrome-c apocytochrome-c-lyase (heme-forming). Other names in common use include cytochrome c heme-lyase, holocytochrome c synthetase, and holocytochrome-c apocytochrome-c-lyase. This enzyme participates in porphyrin and chlorophyll metabolism.

Cytochrome c haem-lyase (CCHL) and cytochrome Cc1 haem-lyase (CC1HL) are mitochondrial enzymes that catalyse the covalent attachment of a haem group on two cysteine residues of cytochrome c and c1. These two enzymes are functionally and evolutionary related. There are two conserved regions, the first is located in the central section and the second in the C-terminal section. Both patterns contain conserved histidine, tryptophan and acidic residues which could be important for the interaction of the enzymes with the apoproteins and/or the haem group.[1]

The human enzyme, HCCS, processes both cytochromes c and c1.[2]

## References

1. ^ Zollner A, Rodel G, Haid A (August 1992). "Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochrome-c1-heme lyase". Eur. J. Biochem. 207 (3): 1093â€“100. doi:10.1111/j.1432-1033.1992.tb17146.x. PMID 1499554.
2. ^ Bernard DG, Gabilly ST, Dujardin G, Merchant S, Hamel PP (December 2003). "Overlapping specificities of the mitochondrial cytochrome c and c1 heme lyases". The Journal of Biological Chemistry. 278 (50): 49732â€“42. doi:10.1074/jbc.M308881200. PMID 14514677.
This article incorporates text from the public domain Pfam and InterPro: IPR000511

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

# Cytochrome c/c1 heme lyase

No Pfam abstract.

This tab holds annotation information from the InterPro database.

# InterPro entry IPR000511

Cytochrome c haem-lyase (CCHL) ( EC ) and cytochrome Cc1 haem-lyase (CC1HL) [ PUBMED:1499554 ] are mitochondrial enzymes that catalyse the covalent attachment of a haem group on two cysteine residues of cytochrome c and c1. These two enzymes are functionally and evolutionary related. There are two conserved regions, the first is located in the central section and the second in the C-terminal section. Both patterns contain conserved histidine, tryptophan and acidic residues which could be important for the interaction of the enzymes with the apoproteins and/or the haem group.

### Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

# Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

# Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

## View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Seed
(56)
Full
(2308)
Representative proteomes UniProt
(4201)
RP15
(497)
RP35
(1088)
RP55
(1752)
RP75
(2376)
Jalview View  View  View  View  View  View  View
HTML View  View
PP/heatmap 1 View

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: available, not generated, not available.

## Format an alignment

Seed
(56)
Full
(2308)
Representative proteomes UniProt
(4201)
RP15
(497)
RP35
(1088)
RP55
(1752)
RP75
(2376)
Alignment:
Format:
Order:
Sequence:
Gaps:

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Seed
(56)
Full
(2308)
Representative proteomes UniProt
(4201)
RP15
(497)
RP35
(1088)
RP55
(1752)
RP75
(2376)

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

# HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

# Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

# Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

## Curation

 Seed source: Prosite Previous IDs: none Type: Family Sequence Ontology: SO:0100021 Author: Finn RD , Bateman A Number in seed: 56 Number in full: 2308 Average length of the domain: 231.20 aa Average identity of full alignment: 36 % Average coverage of the sequence by the domain: 80.47 %

## HMM information

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.8 23.8
Trusted cut-off 23.9 23.8
Noise cut-off 21.7 23.6
Model length: 298
Family (HMM) version: 19

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### Colour assignments

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...