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1  structure 705  species 0  interactions 3349  sequences 102  architectures

Family: Peptidase_M8 (PF01457)

Summary: Leishmanolysin

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Leishmanolysin Provide feedback

No Pfam abstract.

Literature references

  1. Rawlings ND, Barrett AJ; , Meth Enzymol 1995;248:183-228.: Evolutionary families of metallopeptidases. PUBMED:7674922 EPMC:7674922


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001577

This group of metallopeptidases belong to the MEROPS peptidase family M8 (leishmanolysin family, clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA.

Leishmanolysin ( EC ) is an enzyme found in eukaryotes including Leishmania and related parasitic protozoa [ PUBMED:7674922 ]. The endopeptidase is the most abundant protein on the cell surface during the promastigote stage of the parasite, and is attached to the membrane by a glycosylphosphatidylinositol anchor [ PUBMED:7674922 ]. In the amastigote form, the parasite lives in lysosomes of host macrophages, producing a form of the protease that has an acidic pH optimum [ PUBMED:7674922 ]. This differs from most other metalloproteases and may be an adaptation to the environment in which the organism survives [ PUBMED:7674922 ].

Homologues are known from human (leishmanolysin-like peptidase or invadolysin [ PUBMED:19706689 ] and leishmanolysin-like peptidase 2) and other chordates, but have not been well-characterized

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Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(8)
Full
(3349)
Representative proteomes UniProt
(7192)
RP15
(923)
RP35
(1630)
RP55
(2617)
RP75
(3576)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(8)
Full
(3349)
Representative proteomes UniProt
(7192)
RP15
(923)
RP35
(1630)
RP55
(2617)
RP75
(3576)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(8)
Full
(3349)
Representative proteomes UniProt
(7192)
RP15
(923)
RP35
(1630)
RP55
(2617)
RP75
(3576)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prodom_3085 (release 99.1)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 8
Number in full: 3349
Average length of the domain: 294.80 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 61.21 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.3 19.3
Trusted cut-off 19.3 19.3
Noise cut-off 19.2 19.2
Model length: 529
Family (HMM) version: 18
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
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Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Peptidase_M8 domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2JX27 View 3D Structure Click here
A0A0R0JBF1 View 3D Structure Click here
A0A1B0GTW7 View 3D Structure Click here
A0A1D6L355 View 3D Structure Click here
A0A286YEC0 View 3D Structure Click here
A4HUF6 View 3D Structure Click here
A4HUF8 View 3D Structure Click here
A4HUF9 View 3D Structure Click here
A4HUG0 View 3D Structure Click here
A4I6X5 View 3D Structure Click here
D4AC69 View 3D Structure Click here
D4AC69 View 3D Structure Click here
E9AHH5 View 3D Structure Click here
F1Q5N1 View 3D Structure Click here
F4K306 View 3D Structure Click here
I1JTD8 View 3D Structure Click here
I1M8F6 View 3D Structure Click here
I1MXU3 View 3D Structure Click here
O62446 View 3D Structure Click here
Q4CL37 View 3D Structure Click here
Q4CL62 View 3D Structure Click here
Q4CL98 View 3D Structure Click here
Q4CLV4 View 3D Structure Click here
Q4CM21 View 3D Structure Click here
Q4CM26 View 3D Structure Click here
Q4CM87 View 3D Structure Click here
Q4CM87 View 3D Structure Click here
Q4CMC3 View 3D Structure Click here
Q4CMN8 View 3D Structure Click here
Q4CMN9 View 3D Structure Click here
Q4CMP1 View 3D Structure Click here
Q4CMP5 View 3D Structure Click here
Q4CMY9 View 3D Structure Click here
Q4CMZ0 View 3D Structure Click here
Q4CN42 View 3D Structure Click here
Q4CNB7 View 3D Structure Click here
Q4CNI9 View 3D Structure Click here
Q4CNJ0 View 3D Structure Click here
Q4CNW0 View 3D Structure Click here
Q4CNY8 View 3D Structure Click here
Q4CP23 View 3D Structure Click here
Q4CP24 View 3D Structure Click here
Q4CP46 View 3D Structure Click here
Q4CPA1 View 3D Structure Click here
Q4CPE3 View 3D Structure Click here
Q4CPE5 View 3D Structure Click here
Q4CPF0 View 3D Structure Click here
Q4CPJ5 View 3D Structure Click here
Q4CPL5 View 3D Structure Click here
Q4CPW7 View 3D Structure Click here
Q4CPY5 View 3D Structure Click here
Q4CQ27 View 3D Structure Click here
Q4CQ30 View 3D Structure Click here
Q4CQ32 View 3D Structure Click here
Q4CQ77 View 3D Structure Click here
Q4CQA7 View 3D Structure Click here
Q4CQF7 View 3D Structure Click here
Q4CQL6 View 3D Structure Click here
Q4CQQ7 View 3D Structure Click here
Q4CQX8 View 3D Structure Click here
Q4CQX9 View 3D Structure Click here
Q4CQZ5 View 3D Structure Click here
Q4CR26 View 3D Structure Click here
Q4CRA0 View 3D Structure Click here
Q4CRU1 View 3D Structure Click here
Q4CSF6 View 3D Structure Click here
Q4CSR3 View 3D Structure Click here
Q4CSU6 View 3D Structure Click here
Q4CT32 View 3D Structure Click here
Q4CTV4 View 3D Structure Click here
Q4CTY9 View 3D Structure Click here
Q4CTZ0 View 3D Structure Click here
Q4CTZ1 View 3D Structure Click here
Q4CV39 View 3D Structure Click here
Q4CVM5 View 3D Structure Click here
Q4CVQ7 View 3D Structure Click here
Q4CW72 View 3D Structure Click here
Q4CW93 View 3D Structure Click here
Q4CWF2 View 3D Structure Click here
Q4CWF3 View 3D Structure Click here
Q4CWX0 View 3D Structure Click here
Q4CWX0 View 3D Structure Click here
Q4CYB1 View 3D Structure Click here
Q4CYI5 View 3D Structure Click here
Q4CYJ4 View 3D Structure Click here
Q4CYM9 View 3D Structure Click here
Q4CZF9 View 3D Structure Click here
Q4D0A5 View 3D Structure Click here
Q4D148 View 3D Structure Click here
Q4D1E6 View 3D Structure Click here
Q4D273 View 3D Structure Click here
Q4D292 View 3D Structure Click here
Q4D2J1 View 3D Structure Click here
Q4D331 View 3D Structure Click here
Q4D336 View 3D Structure Click here
Q4D3M6 View 3D Structure Click here
Q4D3Q5 View 3D Structure Click here
Q4D3Q5 View 3D Structure Click here
Q4D598 View 3D Structure Click here
Q4D5G1 View 3D Structure Click here
Q4D5M5 View 3D Structure Click here
Q4D6L3 View 3D Structure Click here
Q4D6Y3 View 3D Structure Click here
Q4D6Z1 View 3D Structure Click here
Q4D6Z1 View 3D Structure Click here
Q4D738 View 3D Structure Click here
Q4D739 View 3D Structure Click here
Q4D785 View 3D Structure Click here
Q4D7E7 View 3D Structure Click here
Q4D7L1 View 3D Structure Click here
Q4D8S9 View 3D Structure Click here
Q4D8W6 View 3D Structure Click here
Q4D8W7 View 3D Structure Click here
Q4D958 View 3D Structure Click here
Q4D9Z8 View 3D Structure Click here
Q4DAC1 View 3D Structure Click here
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Q4DAG2 View 3D Structure Click here
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Q4DAH2 View 3D Structure Click here
Q4DAK0 View 3D Structure Click here
Q4DC78 View 3D Structure Click here
Q4DC84 View 3D Structure Click here
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Q4DE44 View 3D Structure Click here
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Q4DE95 View 3D Structure Click here
Q4DEM7 View 3D Structure Click here
Q4DF53 View 3D Structure Click here
Q4DG31 View 3D Structure Click here
Q4DG36 View 3D Structure Click here
Q4DGK2 View 3D Structure Click here
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Q4DGK6 View 3D Structure Click here
Q4DHC2 View 3D Structure Click here
Q4DHC2 View 3D Structure Click here
Q4DHC3 View 3D Structure Click here
Q4DHM7 View 3D Structure Click here
Q4DL97 View 3D Structure Click here
Q4DLF6 View 3D Structure Click here
Q4DLP8 View 3D Structure Click here
Q4DM64 View 3D Structure Click here
Q4DMD2 View 3D Structure Click here
Q4DMD2 View 3D Structure Click here
Q4DMQ1 View 3D Structure Click here
Q4DN56 View 3D Structure Click here
Q4DNA5 View 3D Structure Click here
Q4DNA5 View 3D Structure Click here
Q4DNB6 View 3D Structure Click here
Q4DPF5 View 3D Structure Click here
Q4DPK6 View 3D Structure Click here
Q4DR08 View 3D Structure Click here
Q4DS21 View 3D Structure Click here
Q4DTN7 View 3D Structure Click here
Q4DTV2 View 3D Structure Click here
Q4DTZ3 View 3D Structure Click here
Q4DU25 View 3D Structure Click here
Q4DUP0 View 3D Structure Click here
Q4DV20 View 3D Structure Click here
Q4DV60 View 3D Structure Click here
Q4DVI5 View 3D Structure Click here
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Q4DW73 View 3D Structure Click here
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Q4DYE3 View 3D Structure Click here
Q4DZ07 View 3D Structure Click here
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Q4DZ17 View 3D Structure Click here
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Q4DZ18 View 3D Structure Click here
Q4DZ18 View 3D Structure Click here
Q4E047 View 3D Structure Click here
Q4E067 View 3D Structure Click here
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Q4E0N3 View 3D Structure Click here
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Q4E304 View 3D Structure Click here
Q4E3Q7 View 3D Structure Click here
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Q4E451 View 3D Structure Click here
Q4E465 View 3D Structure Click here
Q4E479 View 3D Structure Click here
Q4E4Y1 View 3D Structure Click here
Q4E5D3 View 3D Structure Click here
Q54LN5 View 3D Structure Click here
Q54SQ4 View 3D Structure Click here
Q54VU8 View 3D Structure Click here
Q55GT0 View 3D Structure Click here
Q6AVG4 View 3D Structure Click here
Q6LA77 View 3D Structure Click here
Q6TU47 View 3D Structure Click here
Q8BMN4 View 3D Structure Click here
Q8BMN4 View 3D Structure Click here
Q96KR4 View 3D Structure Click here
Q96KR4 View 3D Structure Click here
Q96KR4 View 3D Structure Click here
Q9VH19 View 3D Structure Click here