Summary: Peptidase family M20/M25/M40
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Peptidase family M20/M25/M40 Provide feedback
This family includes a range of zinc metallopeptidases belonging to several families in the peptidase classification [1]. Family M20 are Glutamate carboxypeptidases. Peptidase family M25 contains X-His dipeptidases.
Literature references
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Rawlings ND, Barrett AJ; , Meth Enzymol 1995;248:183-228.: Evolutionary families of metallopeptidases. PUBMED:7674922 EPMC:7674922
Internal database links
SCOOP: | DUF4910 fn3 M20_dimer Nicastrin P_proprotein PDZ_2 PDZ_6 Peptidase_M18 Peptidase_M28 Peptidase_M42 Por_Secre_tail PPC TFR_dimer |
Similarity to PfamA using HHSearch: | Peptidase_M28 M20_dimer |
External database links
HOMSTRAD: | apbact |
MEROPS: | M20 |
PROSITE: | PDOC00613 |
SCOP: | 1cg2 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR002933
This group of proteins contains the metallopeptidases and non-peptidase homologues (amidohydrolases) that belong to the MEROPS peptidase family M20 (clan MH) [PUBMED:7674922]. The peptidases of this clan have two catalytic zinc ions at the active site, bound by His/Asp, Asp, Glu, Asp/Glu and His. The catalysed reaction involves the release of an N-terminal amino acid, usually neutral or hydrophobic, from a polypeptide [PUBMED:7674922]. The peptidase M20 family has four subfamilies:
- M20A - type example, glutamate carboxypeptidase from Pseudomonas sp. RS16 (SWISSPROT)
- M20B - type example, peptidase T from Escherichia coli (SWISSPROT)
- M20C - type example, X-His dipeptidase from E. coli (SWISSPROT)
- M20D - type example, carboxypeptidase Ss1 from Sulfolobus solfataricus (SWISSPROT)
Homologues from the family that are not peptidases include:
- Acetylornithine deacetylase, which releases an acetyl group and L-ornithine from N(2)-acetyl-L-ornithine [PUBMED:10684608]
- N-acetyldiaminopimelate deacetylase, which catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate [PUBMED:5411754]
- N-carbamoyl-L-amino-acid hydrolase, which converts D,L-5-monosubstituted hydantoins into D- or L-amino acids [PUBMED:1732229]
- Aminoacylase-1, which hydrolyses N-acylated or N-acetylated amino acids [PUBMED:12933810]
- Succinyl-diaminopimelate desuccinylase (EC:3.5.1.18), which catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid, forming succinate and LL-2,6-diaminoheptanedioate (DAP, a component of bacterial cell walls) [PUBMED:3276674]
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | hydrolase activity (GO:0016787) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan Peptidase_MH (CL0035), which has the following description:
This clan contains peptidases belonging to MEROPS clan MH, MC and MF. We also include Nicastrin that is part of the gamma secretase complex and not known to be a peptidase.
The clan contains the following 17 members:
Amidase_3 AstE_AspA DUF2817 DUF4910 FGase Gamma_PGA_hydro Glycolytic Ncstrn_small Nicastrin Peptidase_M14 Peptidase_M17 Peptidase_M18 Peptidase_M20 Peptidase_M28 Peptidase_M42 Peptidase_M99 SpoIIPAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (360) |
Full (57543) |
Representative proteomes | UniProt (242625) |
NCBI (413444) |
Meta (9961) |
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RP15 (6073) |
RP35 (25560) |
RP55 (55301) |
RP75 (97166) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
— not available.
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (360) |
Full (57543) |
Representative proteomes | UniProt (242625) |
NCBI (413444) |
Meta (9961) |
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---|---|---|---|---|---|---|---|---|---|
RP15 (6073) |
RP35 (25560) |
RP55 (55301) |
RP75 (97166) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_253 (release 4.0) |
Previous IDs: | CO_pept_M20; |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Bateman A |
Number in seed: | 360 |
Number in full: | 57543 |
Average length of the domain: | 323.10 aa |
Average identity of full alignment: | 14 % |
Average coverage of the sequence by the domain: | 75.30 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null --hand HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 207 | ||||||||||||
Family (HMM) version: | 29 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
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Interactions
Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Peptidase_M20 domain has been found. There are 170 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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