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10  structures 1691  species 0  interactions 5458  sequences 90  architectures

Family: NAC (PF01849)

Summary: NAC domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

NAC domain Provide feedback

No Pfam abstract.

Literature references

  1. Makarova KS, Aravind L, Galperin MY, Grishin NV, Tatusov RL, Wolf YI, Koonin EV; , Genome Res 1999;9:608-628.: Comparative genomics of the Archaea (Euryarchaeota): evolution of conserved protein families, the stable core, and the variable shell. PUBMED:10413400 EPMC:10413400


This tab holds annotation information from the InterPro database.

InterPro entry IPR002715

In eukaryotes, the Nascent polypeptide-Associated Complex (NAC) is a heterodimeric cytosolic protein complex composed of NAC alpha and NAC beta. NAC binds reversibly to the ribosome where it is in contact with nascent chains as they emerge from the ribosome. But the cellular function of NAC seems to be much more diverse as it is also involved in transcription regulation and mitochondrial translocation [ PUBMED:12475173 ]. Alpha and beta NACs share homology with each other, both contain a NAC A/B domain. In archaea no beta NAC proteins are found; the complex is an homodimer of NAC alpha [ PUBMED:10413400 , PUBMED:15665334 ].

The crystal structure of an archeal NAC has been solved [ PUBMED:15665334 ]. The NAC A/B domain consists of six strands arranged in a beta barrel structure similar to the OB fold. Various OB folds interact with ribosomal RNA which could suggest a similar role for the NAC A/B domain.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(210)
Full
(5458)
Representative proteomes UniProt
(10960)
RP15
(912)
RP35
(2371)
RP55
(4329)
RP75
(5986)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(210)
Full
(5458)
Representative proteomes UniProt
(10960)
RP15
(912)
RP35
(2371)
RP55
(4329)
RP75
(5986)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(210)
Full
(5458)
Representative proteomes UniProt
(10960)
RP15
(912)
RP35
(2371)
RP55
(4329)
RP75
(5986)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: [1]
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 210
Number in full: 5458
Average length of the domain: 55.90 aa
Average identity of full alignment: 40 %
Average coverage of the sequence by the domain: 20.72 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.3 21.3
Trusted cut-off 21.4 21.4
Noise cut-off 21.2 21.2
Model length: 57
Family (HMM) version: 20
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the NAC domain has been found. There are 10 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2JZC7 View 3D Structure Click here
A0A0G2K3P1 View 3D Structure Click here
A0A0G2K4W2 View 3D Structure Click here
A0A0G2K5C5 View 3D Structure Click here
A0A0G2K8T5 View 3D Structure Click here
A0A0P0V707 View 3D Structure Click here
A0A0R0EWX9 View 3D Structure Click here
A0A0R0H141 View 3D Structure Click here
A0A0R4J4H3 View 3D Structure Click here
A0A1D6K372 View 3D Structure Click here
A4HS72 View 3D Structure Click here
A4HS73 View 3D Structure Click here
A4ID19 View 3D Structure Click here
B4FFP6 View 3D Structure Click here
B4FIE5 View 3D Structure Click here
B4FL49 View 3D Structure Click here
B4FL79 View 3D Structure Click here
B6SU89 View 3D Structure Click here
B6SVI7 View 3D Structure Click here
B6TBD4 View 3D Structure Click here
B6VQA0 View 3D Structure Click here
B9FWP2 View 3D Structure Click here
C6KT55 View 3D Structure Click here
C6SX83 View 3D Structure Click here
C6T2G5 View 3D Structure Click here
D4A3I4 View 3D Structure Click here
E7EZQ7 View 3D Structure Click here
E9PAV3 View 3D Structure Click here
F1M6E5 View 3D Structure Click here
I1JMZ3 View 3D Structure Click here
I1JMZ4 View 3D Structure Click here
I1JYG6 View 3D Structure Click here
I1JYG7 View 3D Structure Click here
I1KB25 View 3D Structure Click here
I1KB26 View 3D Structure Click here
I1N8M4 View 3D Structure Click here
K7VIA7 View 3D Structure Click here
M0R5M7 View 3D Structure Click here
M0R9L0 View 3D Structure Click here
O15069 View 3D Structure Click here
P20290 View 3D Structure Click here
P38879 View 3D Structure Click here
P40314 View 3D Structure Click here
P70670 View 3D Structure Click here
P87147 View 3D Structure Click here
Q02642 View 3D Structure Click here
Q08C78 View 3D Structure Click here
Q18885 View 3D Structure Click here
Q4CYN3 View 3D Structure Click here
Q4D103 View 3D Structure Click here
Q4D9L7 View 3D Structure Click here
Q4DD49 View 3D Structure Click here
Q54TR8 View 3D Structure Click here
Q54U07 View 3D Structure Click here
Q57728 View 3D Structure Click here
Q59TU0 View 3D Structure Click here
Q5ANP2 View 3D Structure Click here
Q5SWP3 View 3D Structure Click here
Q5U3Y8 View 3D Structure Click here
Q64152 View 3D Structure Click here
Q6ICZ8 View 3D Structure Click here
Q6PC91 View 3D Structure Click here
Q7KM15 View 3D Structure Click here
Q7XXR8 View 3D Structure Click here
Q84VF8 View 3D Structure Click here
Q851Y9 View 3D Structure Click here
Q86S66 View 3D Structure Click here
Q8ILK2 View 3D Structure Click here
Q8IR50 View 3D Structure Click here
Q8JIU7 View 3D Structure Click here
Q8LGC6 View 3D Structure Click here
Q8LMR3 View 3D Structure Click here
Q8LNW1 View 3D Structure Click here
Q8RUI4 View 3D Structure Click here
Q92371 View 3D Structure Click here
Q94518 View 3D Structure Click here
Q94JX9 View 3D Structure Click here
Q96K17 View 3D Structure Click here
Q9BZK3 View 3D Structure Click here
Q9CAT7 View 3D Structure Click here
Q9CQH7 View 3D Structure Click here
Q9H009 View 3D Structure Click here
Q9LHG9 View 3D Structure Click here
Q9SMW7 View 3D Structure Click here
Q9SZY1 View 3D Structure Click here
Q9VPR2 View 3D Structure Click here
Q9VPV7 View 3D Structure Click here
Q9VY34 View 3D Structure Click here
Q9VY38 View 3D Structure Click here
Q9VY63 View 3D Structure Click here