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5  structures 1302  species 0  interactions 5152  sequences 70  architectures

Family: KA1 (PF02149)

Summary: Kinase associated domain 1

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This is the Wikipedia entry entitled "KIN2/PAR-1/MARK kinase family". More...

KIN2/PAR-1/MARK kinase family Edit Wikipedia article

Kinase associated domain 1
PDB 1ul7 EBI.jpg
solution structure of kinase associated domain 1 of mouse map/microtubule affinity-regulating kinase 3

In molecular biology, members of the KIN2/PAR-1/MARK kinase family of proteins are kinases that are conserved from yeast to human and share the same domain organisation: an N-terminal kinase domain and a C-terminal kinase associated domain 1 (KA1). Some members of this family also contain an UBA domain (ubiquitin-associated domain). Members of this kinase family are involved in various biological processes such as cell polarity, cell cycle control, intracellular signalling, microtubule stability and protein stability.[1] The function of the KA1 domain is not yet known.

Some proteins known to contain a KA1 domain are listed below:


  1. ^ Tassan JP, Le Goff X (April 2004). "An overview of the KIN1/PAR-1/MARK kinase family". Biol. Cell. 96 (3): 193–9. doi:10.1016/j.biolcel.2003.10.009. PMID 15182702.
  2. ^ Biernat J, Wu YZ, Timm T, Zheng-Fischhofer Q, Mandelkow E, Meijer L, Mandelkow EM (November 2002). "Protein kinase MARK/PAR-1 is required for neurite outgrowth and establishment of neuronal polarity". Mol. Biol. Cell. 13 (11): 4013–28. doi:10.1091/mbc.02-03-0046. PMC 133611. PMID 12429843.
  3. ^ Guo S, Kemphues KJ (May 1995). "par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed". Cell. 81 (4): 611–20. doi:10.1016/0092-8674(95)90082-9. PMID 7758115.
  4. ^ Elbert M, Rossi G, Brennwald P (February 2005). "The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery". Mol. Biol. Cell. 16 (2): 532–49. doi:10.1091/mbc.E04-07-0549. PMC 545889. PMID 15563607.
This article incorporates text from the public domain Pfam and InterPro: IPR001772

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Kinase associated domain 1 Provide feedback

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External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001772

Members of the KIN2/PAR-1/MARK kinase subfamily are conserved from yeast to human and share the same domain organisation: an N-terminal kinase domain ( INTERPRO ) and a C-terminal kinase associated domain 1 (KA1). Some members of the KIN1/PAR-1/MARK family also contain an UBA domain ( INTERPRO ). Members of this kinase subfamily are involved in various biological processes such as cell polarity, cell cycle control, intracellular signalling, microtubule stability and protein stability [ PUBMED:15182702 ]. The function of the KA1 domain is not yet known but several studies strongly suggest that it is involved in protein localisation. In addition, it has been reported that this C-terminal region acts as an autoinhibitory domain for the N‐terminal kinase domain [ PUBMED:17075132 ].

Some proteins known to contain a KA1 domain are listed below:
  • Mammalian MAP/microtubule affinity-regulating kinases (MARK 1,2,3). They regulate polarity in neuronal cell models and appear to function redundantly in phosphorylating MT-associated proteins and in regulating MT stability [ PUBMED:12429843 ].
  • Mammalian maternal embryonic leucine zipper kinase (MELK). It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may be involved in the inhibition of spliceosome assembly during mitosis.
  • Caenorhabditis elegans and drosophila PAR-1 protein. It is required for establishing polarity in embryos where it is asymmetrically distributed [ PUBMED:7758115 ].
  • Fungal Kin1 and Kin2 protein kinases involved in regulation of exocytosis. They localise to the cytoplasmic face of the plasma membrane [ PUBMED:15563607 ].

The KA1 domain comprises about 50 amino acid residues and end in the highly conserved Glu‐Leu‐Lys‐Leu motif, termed the ELKL motif which forms a concave surface surrounded by positively charged residues, being important for the KA1 domain function. This domain adopts a compact alpha+beta structure with a beta-alpha-beta-beta-beta-beta-alpha topology [ PUBMED:17075132 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan KA1-like (CL0573), which has the following description:

This superfamily is characterised by small adenylate-sensing domains at the C-terminus of molecules such as AMP-Activated Protein Kinases an SNF1-like protein kinases [1].

The clan contains the following 4 members:

AdenylateSensor KA1 NAF NAM-associated


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: IPR001772
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Mian N , Bateman A
Number in seed: 82
Number in full: 5152
Average length of the domain: 44.60 aa
Average identity of full alignment: 43 %
Average coverage of the sequence by the domain: 5.83 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.8 20.8
Trusted cut-off 20.8 20.8
Noise cut-off 20.7 20.7
Model length: 45
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the KA1 domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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