Summary: Nitrile hydratase beta subunit
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Nitrile hydratase Edit Wikipedia article
Nitrile hydratase and amidase are the two hydrolytic enzymes responsible for the sequential metabolism of nitriles in some bacteria and fungi which are capable of utilising aliphatic nitriles as the sole source of nitrogen and carbon. Nitrile hydratases (NHases; EC 188.8.131.52) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of a large number of diverse nitriles to their corresponding amides in a reaction
R-Câ‰¡N + H2O â†’ R-C(O)NH2
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Nitrile hydratase beta subunit Provide feedback
Nitrile hydratases EC:184.108.40.206 are unusual metalloenzymes that catalyse the hydration of nitriles to their corresponding amides. They are used as biocatalysts in acrylamide production, one of the few commercial scale bioprocesses, as well as in environmental remediation for the removal of nitriles from waste streams. Nitrile hydratases are composed of two subunits, alpha and beta, and they contain one iron atom per alpha beta unit .
Huang W, Jia J, Cummings J, Nelson M, Schneider G, Lindqvist Y; , Structure 1997;5:691-699.: Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold. PUBMED:9195885 EPMC:9195885
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR024690
Nitrile hydratases ( EC ) are unusual metalloenzymes that catalyse the hydration of nitriles to their corresponding amides. They are used as biocatalysts in acrylamide production, one of the few commercial scale bioprocesses, as well as in environmental remediation for the removal of nitriles from waste streams. Nitrile hydratases are composed of two subunits, alpha and beta, and they contain one iron atom per alpha beta unit [ PUBMED:9195885 ].
This entry represents the structural domain of nitrile hydratase beta subunit which contains irregular array of helices in the N-terminal extension.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||nitrile hydratase activity (GO:0018822)|
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
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|Seed source:||Pfam-B_5347 (release 5.2)|
|Number in seed:||40|
|Number in full:||1621|
|Average length of the domain:||151 aa|
|Average identity of full alignment:||22 %|
|Average coverage of the sequence by the domain:||92.96 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||18|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the NHase_beta domain has been found. There are 132 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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