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5  structures 6734  species 0  interactions 8870  sequences 9  architectures

# Summary: Bacitracin resistance protein BacA

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This is the Wikipedia entry entitled "Undecaprenyl-diphosphatase". More...

# Undecaprenyl-diphosphatase

In enzymology, an undecaprenyl-diphosphatase (EC 3.6.1.27) is an enzyme that catalyzes the chemical reaction

undecaprenyl diphosphate + H2O ${\displaystyle \rightleftharpoons }$ undecaprenyl phosphate + phosphate

Thus, the two substrates of this enzyme are undecaprenyl diphosphate and H2O, whereas its two products are undecaprenyl phosphate and phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is undecaprenyl-diphosphate phosphohydrolase. Other names in common use include C55-isoprenyl diphosphatase, C55-isoprenyl pyrophosphatase, and isoprenyl pyrophosphatase. This enzyme participates in peptidoglycan biosynthesis.

## References

• Goldman R, Strominger JL (1972). "Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus". J. Biol. Chem. 247: 5116â€“22. PMIDÂ 4341539.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

# Bacitracin resistance protein BacA

Bacitracin resistance protein (BacA) is a putative undecaprenol kinase. BacA confers resistance to bacitracin, probably by phosphorylation of undecaprenol [1]. More recent studies show that BacA has undecaprenyl pyrophosphate phosphatase activity. Undecaprenyl phosphate is a key lipid intermediate involved in the synthesis of various bacterial cell wall polymers. Bacitracin, a mixture of related cyclic polypeptide antibiotics, is used to treat surface tissue infections. Its primary mode of action is the inhibition of bacterial cell wall synthesis through sequestration of the essential carrier lipid undecaprenyl pyrophosphate, C55-PP, resulting in the loss of cell integrity and lysis [2,3]. The characteristic phosphatase sequence-motif in this family is likely to be the PGxSRSGG, compared with the PSGH of the PAP family of phosphatases [3].

## Literature references

1. Cain BD, Norton PJ, Eubanks W, Nick HS, Allen CM; , J Bacteriol 1993;175:3784-3789.: Amplification of the bacA gene confers bacitracin resistance to Escherichia coli. PUBMED:8389741 EPMC:8389741

2. El Ghachi M, Bouhss A, Blanot D, Mengin-Lecreulx D;, J Biol Chem. 2004;279:30106-30113.: The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity. PUBMED:15138271 EPMC:15138271

3. El Ghachi M, Derbise A, Bouhss A, Mengin-Lecreulx D;, J Biol Chem. 2005;280:18689-18695.: Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli. PUBMED:15778224 EPMC:15778224

This tab holds annotation information from the InterPro database.

# InterPro entry IPR003824

This is a family of small, highly hydrophobic proteins. Over-expression of this protein in Escherichia coli is associated with bacitracin resistance [ PUBMED:8389741 ], and the protein was originally proposed to be an undecaprenol kinase called bacA. BacA protein, however, does not show undecaprenol phosphokinase activity [ PUBMED:15138271 ]. It is now known to be an undecaprenyl pyrophosphate phosphatase ( EC ) and is renamed UppP. It is not the only protein associated with bacitracin resistance [ PUBMED:15946938 , PUBMED:15778224 ].

### Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

# Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

# Pfam Clan

This family is a member of clan LysE (CL0292), which has the following description:

This clan includes a diverse range of transporter families [1].

The clan contains the following 19 members:

BacA

# Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

## View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Seed
(815)
Full
(8870)
Representative proteomes UniProt
(45642)
RP15
(1149)
RP35
(4450)
RP55
(9289)
RP75
(16132)
Jalview View  View  View  View  View  View  View
HTML View
PP/heatmap 1

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: available, not generated, not available.

## Format an alignment

Seed
(815)
Full
(8870)
Representative proteomes UniProt
(45642)
RP15
(1149)
RP35
(4450)
RP55
(9289)
RP75
(16132)
Alignment:
Format:
Order:
Sequence:
Gaps:

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Seed
(815)
Full
(8870)
Representative proteomes UniProt
(45642)
RP15
(1149)
RP35
(4450)
RP55
(9289)
RP75
(16132)

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

# HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

# Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

# Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

## Curation

 Seed source: COG1968 Previous IDs: none Type: Family Sequence Ontology: SO:0100021 Author: Mian N , Bateman A Number in seed: 815 Number in full: 8870 Average length of the domain: 255 aa Average identity of full alignment: 34 % Average coverage of the sequence by the domain: 93.23 %

## HMM information

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 33.4 33.4
Trusted cut-off 33.7 33.9
Noise cut-off 33.0 33.0
Model length: 256
Family (HMM) version: 21

# Species distribution

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### Colour assignments

 Archea Eukaryota Bacteria Other sequences Viruses Unclassified Viroids Unclassified sequence

### Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

### Tree controls

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# Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BacA domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

# AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information