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192  structures 1124  species 0  interactions 8937  sequences 229  architectures

Family: Myosin_N (PF02736)

Summary: Myosin N-terminal SH3-like domain

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Myosin N-terminal SH3-like domain Provide feedback

This domain has an SH3-like fold. It is found at the N-terminus of many but not all myosins. The function of this domain is unknown.

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR004009

Members of the myosin superfamily of actin-based motors act in a variety of cellular functions such as muscle contraction, cell and organelle movement, membrane trafficking, and signal transduction. Although myosin motor domains show a high degree of sequence conservation, the individual myosin classes are clearly defined by differences in the head structure. The N-terminal region of myosins from different classes varies greatly in length and amino acid composition among the individual members. Many myosins have an SH3-like domain at the N terminus of the motor domain. This includes myosins in classes II, V, VI, XI, XXII and XXIV. The myosin N-terminal SH3-like domain may mediate some aspect of the conformational communication that occurs within the myosin head during actin and nucleotide binding. Part of this effect may be mediated through interactions with the neck-associated essential light chains that are in close proximity to this portion of the head domain and also transiently interact with actin [ PUBMED:16982629 , PUBMED:17597155 , PUBMED:20217677 ].

The myosin N-terminal SH3-like domain comprises ~50 amino acids and forms a protruding, six-stranded, antiparallel, beta-barrel domain with similarities to the SH3 domain [ PUBMED:16982629 , PUBMED:15944696 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan SH3 (CL0010), which has the following description:

Src homology-3 (SH3) domains are comprised of about 60 amino acids, performing either an assembly or regulatory role. For example, SH3 domains in the Grb2 adaptor protein are essential for protein-protein interactions and signal transduction in the p21 Ras-dependent growth factor signaling pathway. Alternatively, SH3 performs a regulatory role in the Src family of tyrosine kinases. SH3 domains bind a variety of peptide ligands, many of which contain a PxxP motif. This PxxP motif is flanked by different specificity elements [1]. Structures of SH3 domains, both free and ligand complexed, have provided insights into the mechanism of ligand recognition. The SH3 fold consists of two anti-parallel beta sheets that lie at right angles to each other. Within the fold, there are two variable loops, referred to as RT and n-Src loops. When SH3 binds to its ligand, the proline rich ligand adopts a PPII helix conformation, with the PPII helix structure recognised by a pair of grooves on the surface of the SH3 domain that bind turns of the helix. The SH3 grooves are formed by a series of nearly parallel, well-conserved aromatic residues [1].

The clan contains the following 37 members:

CAP_GLY DUF150_C DUF1541 DUF1653 DUF3104 DUF3247 DUF3601 DUF4453 DUF4648 Gemin6 Gemin7 GW hSH3 IN_DBD_C KapB MLVIN_C Myosin_N NdhS SH3_1 SH3_10 SH3_11 SH3_12 SH3_13 SH3_14 SH3_15 SH3_16 SH3_17 SH3_18 SH3_19 SH3_2 SH3_3 SH3_4 SH3_5 SH3_6 SH3_9 SlpA YjdM

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(192)
Full
(8937)
Representative proteomes UniProt
(15060)
RP15
(1029)
RP35
(3316)
RP55
(7768)
RP75
(10377)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(192)
Full
(8937)
Representative proteomes UniProt
(15060)
RP15
(1029)
RP35
(3316)
RP55
(7768)
RP75
(10377)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(192)
Full
(8937)
Representative proteomes UniProt
(15060)
RP15
(1029)
RP35
(3316)
RP55
(7768)
RP75
(10377)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_110 (Release 5.5)
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 192
Number in full: 8937
Average length of the domain: 45.00 aa
Average identity of full alignment: 32 %
Average coverage of the sequence by the domain: 2.74 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.0
Noise cut-off 26.9 26.9
Model length: 45
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Myosin_N domain has been found. There are 192 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2JY53 View 3D Structure Click here
A0A0G2L365 View 3D Structure Click here
A0A0N4STT5 View 3D Structure Click here
A0A0P0W1J8 View 3D Structure Click here
A0A0P0W5U4 View 3D Structure Click here
A0A0P0YCR6 View 3D Structure Click here
A0A0R0E5G3 View 3D Structure Click here
A0A0R0GUH4 View 3D Structure Click here
A0A0R4IHN7 View 3D Structure Click here
A0A0R4IJY6 View 3D Structure Click here
A0A1D6G388 View 3D Structure Click here
A0A1D6GHG7 View 3D Structure Click here
A0A1D6HPF7 View 3D Structure Click here
A0A1D6HXR0 View 3D Structure Click here
A0A1D6LCG3 View 3D Structure Click here
A0A1D6MB87 View 3D Structure Click here
A0A1D6NE40 View 3D Structure Click here
A0A1D6NKQ5 View 3D Structure Click here
A0A1D6PNU9 View 3D Structure Click here
A0A2R8PYV3 View 3D Structure Click here
A0A2R8Q7G9 View 3D Structure Click here
A0A2R8Q9M0 View 3D Structure Click here
A0A2R8QHZ3 View 3D Structure Click here
A0A2R8QLD9 View 3D Structure Click here
A0A2R8QM44 View 3D Structure Click here
A0A2R8QN33 View 3D Structure Click here
A0A2R8QQT1 View 3D Structure Click here
A2AQP0 View 3D Structure Click here
A2BGX6 View 3D Structure Click here
A7E2Y1 View 3D Structure Click here
B1AR69 View 3D Structure Click here
B3DFS9 View 3D Structure Click here
B6RK61 View 3D Structure Click here
B8A561 View 3D Structure Click here
B8A568 View 3D Structure Click here
B8A569 View 3D Structure Click here
B8JLS3 View 3D Structure Click here
E7F5L1 View 3D Structure Click here
E7FD18 View 3D Structure Click here
E9Q264 View 3D Structure Click here
E9QFE1 View 3D Structure Click here
F1LNF0 View 3D Structure Click here
F1QC64 View 3D Structure Click here
F1QVX3 View 3D Structure Click here
F1QZW1 View 3D Structure Click here
F1R3G4 View 3D Structure Click here
F1R6C7 View 3D Structure Click here
F4HWY6 View 3D Structure Click here
F4HXP9 View 3D Structure Click here
F4I460 View 3D Structure Click here
F4I5Q6 View 3D Structure Click here
F4IRU3 View 3D Structure Click here
F4IUG9 View 3D Structure Click here
F4IVR7 View 3D Structure Click here
F4JM19 View 3D Structure Click here
F4K5J1 View 3D Structure Click here
G3UW82 View 3D Structure Click here
I1JRQ8 View 3D Structure Click here
I1JU19 View 3D Structure Click here
I1KTU1 View 3D Structure Click here
I1LY26 View 3D Structure Click here
I1LYR2 View 3D Structure Click here
I1MJG6 View 3D Structure Click here
I1MSD7 View 3D Structure Click here
I1N9X9 View 3D Structure Click here
I1NIS2 View 3D Structure Click here
K7KFL5 View 3D Structure Click here
K7KTA5 View 3D Structure Click here
K7LGK9 View 3D Structure Click here
K7LHD6 View 3D Structure Click here
K7LJT2 View 3D Structure Click here
K7W4X8 View 3D Structure Click here
O08638 View 3D Structure Click here
O14157 View 3D Structure Click here
O62244 View 3D Structure Click here
P02563 View 3D Structure Click here
P02564 View 3D Structure Click here
P02566 View 3D Structure Click here
P02567 View 3D Structure Click here
P05661 View 3D Structure Click here
P08799 View 3D Structure Click here
P11055 View 3D Structure Click here
P12844 View 3D Structure Click here
P12845 View 3D Structure Click here
P12847 View 3D Structure Click here
P12882 View 3D Structure Click here
P12883 View 3D Structure Click here
P13533 View 3D Structure Click here
P13535 View 3D Structure Click here
P13541 View 3D Structure Click here
P13542 View 3D Structure Click here
P35579 View 3D Structure Click here
P35580 View 3D Structure Click here
P35749 View 3D Structure Click here
Q01989 View 3D Structure Click here
Q02566 View 3D Structure Click here
Q0DGE2 View 3D Structure Click here
Q0DNL1 View 3D Structure Click here
Q0JJV8 View 3D Structure Click here
Q0WPU1 View 3D Structure Click here
Q20641 View 3D Structure Click here
Q21000 View 3D Structure Click here
Q29RW1 View 3D Structure Click here
Q39160 View 3D Structure Click here
Q5SX39 View 3D Structure Click here
Q5SX40 View 3D Structure Click here
Q61879 View 3D Structure Click here
Q62812 View 3D Structure Click here
Q67VX5 View 3D Structure Click here
Q6IQX1 View 3D Structure Click here
Q6URW6 View 3D Structure Click here
Q7Z406 View 3D Structure Click here
Q8VDD5 View 3D Structure Click here
Q91Z83 View 3D Structure Click here
Q99323 View 3D Structure Click here
Q9JLT0 View 3D Structure Click here
Q9LKB9 View 3D Structure Click here
Q9M2K0 View 3D Structure Click here
Q9TZI9 View 3D Structure Click here
Q9UKX2 View 3D Structure Click here
Q9UKX3 View 3D Structure Click here
Q9USI6 View 3D Structure Click here
Q9Y2K3 View 3D Structure Click here
Q9Y623 View 3D Structure Click here