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502  structures 7990  species 0  interactions 46565  sequences 216  architectures

Family: 4HBT (PF03061)

Summary: Thioesterase superfamily

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Thioesterase superfamily Provide feedback

This family contains a wide variety of enzymes, principally thioesterases. This family includes 4HBT (EC 3.1.2.23) which catalyses the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3. This family includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyse the hydrolysis of other long chain fatty acyl-CoA thioesters.

Literature references

  1. Benning MM, Wesenberg G, Liu R, Taylor KL, Dunaway-Mariano D, Holden HM; , J Biol Chem 1998;273:33572-33579.: The three-dimensional structure of 4-hydroxybenzoyl-CoA thioesterase from Pseudomonas sp. Strain CBS-3. PUBMED:9837940 EPMC:9837940

  2. Leesong M, Henderson BS, Gillig JR, Schwab JM, Smith JL; , Structure 1996;4:253-264.: Structure of a dehydratase-isomerase from the bacterial pathway for biosynthesis of unsaturated fatty acids: two catalytic activities in one active site. PUBMED:8805534 EPMC:8805534


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006683

A wide variety of enzymes contain this domain, principally thioesterases. Proteins containing this domain include 4HBT (4-hydroxybenzoyl-CoA thioesterase) ( EC ) which catalyses the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3 [ PUBMED:9837940 , PUBMED:1610806 ]. It also includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyse the hydrolysis of other long chain fatty acyl-CoA thioesters [ PUBMED:8805534 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan HotDog (CL0050), which has the following description:

The HotDog fold was first observed in the structure of Escherichia coli beta-hydroxydecanoyl thiol ester dehydratase (FabA), where Leesong et al. noticed that each subunit of this dimeric enzyme contained a mixed alpha + beta 'hot dog' fold. They described the seven-stranded antiparallel beta-sheet as the 'bun', which wraps around a five-turn alpha-helical 'sausage', This superfamily contains a diverse range of enzymes. Membership includes numerous prokaryotic, archaeal and eukaryotic proteins involved in several related, but distinct, catalytic activities, from metabolic roles such as thioester hydrolysis in fatty acid metabolism, to degradation of phenylacetic acid and the environmental pollutant 4-chlorobenzoate. The superfamily also includes FapR, a non-catalytic bacterial homologue that is involved in transcriptional regulation of fatty acid biosynthesis [1].

The clan contains the following 14 members:

4HBT 4HBT_2 4HBT_3 Acyl-ACP_TE Acyl_CoA_thio AfsA DUF4442 FabA FcoT MaoC_dehydrat_N MaoC_dehydratas MC_hydratase PS-DH YiiD_C

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(125)
Full
(46565)
Representative proteomes UniProt
(184329)
RP15
(4912)
RP35
(19545)
RP55
(45047)
RP75
(79489)
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HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(125)
Full
(46565)
Representative proteomes UniProt
(184329)
RP15
(4912)
RP35
(19545)
RP55
(45047)
RP75
(79489)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(125)
Full
(46565)
Representative proteomes UniProt
(184329)
RP15
(4912)
RP35
(19545)
RP55
(45047)
RP75
(79489)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_2758 (release 6.4)
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 125
Number in full: 46565
Average length of the domain: 79.20 aa
Average identity of full alignment: 17 %
Average coverage of the sequence by the domain: 46.51 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.6 20.6
Trusted cut-off 20.6 20.6
Noise cut-off 20.5 20.5
Model length: 79
Family (HMM) version: 24
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the 4HBT domain has been found. There are 502 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0N7KK42 View 3D Structure Click here
A0A0P0VMI0 View 3D Structure Click here
A0A0P0VMM7 View 3D Structure Click here
A0A0P0X651 View 3D Structure Click here
A0A0R0II97 View 3D Structure Click here
A0A0R0KIT7 View 3D Structure Click here
A0A0R0KY19 View 3D Structure Click here
A0A1D6E313 View 3D Structure Click here
A0A1D6E3W1 View 3D Structure Click here
A0A1D6ET42 View 3D Structure Click here
A0A1D6F200 View 3D Structure Click here
A0A1D6HZ82 View 3D Structure Click here
A0A1D6HZ82 View 3D Structure Click here
A0A1D6JJ43 View 3D Structure Click here
A0A1D6K9H0 View 3D Structure Click here
A0A1D6KJS7 View 3D Structure Click here
A0A1D6MF39 View 3D Structure Click here
A0A1D8PI65 View 3D Structure Click here
A0A2R8QNU6 View 3D Structure Click here
A3A9U2 View 3D Structure Click here
A4I4M9 View 3D Structure Click here
A4ICC1 View 3D Structure Click here
B4F943 View 3D Structure Click here
B6SNT9 View 3D Structure Click here
B6UFQ3 View 3D Structure Click here
C0P6T7 View 3D Structure Click here
C0PDS6 View 3D Structure Click here
C4JAL9 View 3D Structure Click here
C6T483 View 3D Structure Click here
C6T4X8 View 3D Structure Click here
C6TGE4 View 3D Structure Click here
C7G041 View 3D Structure Click here
D3ZA93 View 3D Structure Click here
D3ZHF1 View 3D Structure Click here
D4AEL0 View 3D Structure Click here
E7FCU2 View 3D Structure Click here
E7FCU2 View 3D Structure Click here
E9QJN3 View 3D Structure Click here
E9QJN3 View 3D Structure Click here
F1LX28 View 3D Structure Click here
F1LX28 View 3D Structure Click here
F1Q7G7 View 3D Structure Click here
F1Q7G7 View 3D Structure Click here
F4HX80 View 3D Structure Click here
F8W5J5 View 3D Structure Click here
I1J6J3 View 3D Structure Click here
I1JF18 View 3D Structure Click here
I1JHA4 View 3D Structure Click here
I1JXU2 View 3D Structure Click here
I1KIF8 View 3D Structure Click here
I1KIF9 View 3D Structure Click here
I1KLT7 View 3D Structure Click here
I1L1Z6 View 3D Structure Click here
I1L5W0 View 3D Structure Click here
I1N3I1 View 3D Structure Click here
K7KLB0 View 3D Structure Click here
K7TMF4 View 3D Structure Click here
K7VRF1 View 3D Structure Click here
O00154 View 3D Structure Click here
O00154 View 3D Structure Click here
O06178 View 3D Structure Click here
O06307 View 3D Structure Click here
O07408 View 3D Structure Click here
O74793 View 3D Structure Click here
P0A8Y8 View 3D Structure Click here
P0A8Z0 View 3D Structure Click here
P0A8Z3 View 3D Structure Click here
P0ADP2 View 3D Structure Click here
P34419 View 3D Structure Click here
P38172 View 3D Structure Click here
P76084 View 3D Structure Click here
P77712 View 3D Structure Click here
P77781 View 3D Structure Click here
P87304 View 3D Structure Click here
P93828 View 3D Structure Click here
P9WIM3 View 3D Structure Click here
Q18187 View 3D Structure Click here
Q2FX15 View 3D Structure Click here
Q2FXM2 View 3D Structure Click here
Q2FYS8 View 3D Structure Click here
Q2FZ56 View 3D Structure Click here
Q2FZV4 View 3D Structure Click here
Q3UUI3 View 3D Structure Click here
Q4DYH8 View 3D Structure Click here
Q54D65 View 3D Structure Click here
Q54GL4 View 3D Structure Click here
Q54HX1 View 3D Structure Click here
Q54LP9 View 3D Structure Click here
Q566R0 View 3D Structure Click here
Q59ZH1 View 3D Structure Click here
Q5T1C6 View 3D Structure Click here
Q64559 View 3D Structure Click here
Q64559 View 3D Structure Click here
Q69MT1 View 3D Structure Click here
Q6AV57 View 3D Structure Click here
Q6AWX1 View 3D Structure Click here
Q6ZUV0 View 3D Structure Click here
Q7XIL1 View 3D Structure Click here
Q7XV63 View 3D Structure Click here
Q86HX3 View 3D Structure Click here
Q8IG45 View 3D Structure Click here
Q8N1Q8 View 3D Structure Click here
Q8VHQ9 View 3D Structure Click here
Q8VHQ9 View 3D Structure Click here
Q8W583 View 3D Structure Click here
Q8WXI4 View 3D Structure Click here
Q8WXI4 View 3D Structure Click here
Q8WYK0 View 3D Structure Click here
Q8WYK0 View 3D Structure Click here
Q91V12 View 3D Structure Click here
Q91V12 View 3D Structure Click here
Q940V5 View 3D Structure Click here
Q99NB7 View 3D Structure Click here
Q99NB7 View 3D Structure Click here
Q9C7I5 View 3D Structure Click here
Q9C7I8 View 3D Structure Click here
Q9CQJ0 View 3D Structure Click here
Q9CQR4 View 3D Structure Click here
Q9DBK0 View 3D Structure Click here
Q9DBK0 View 3D Structure Click here
Q9DCP4 View 3D Structure Click here
Q9FI76 View 3D Structure Click here
Q9LWL3 View 3D Structure Click here
Q9M2E4 View 3D Structure Click here
Q9NPJ3 View 3D Structure Click here
Q9SX65 View 3D Structure Click here
Q9VZZ5 View 3D Structure Click here
Q9VZZ6 View 3D Structure Click here
Q9ZW37 View 3D Structure Click here