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31  structures 1138  species 0  interactions 11971  sequences 86  architectures

Family: SLC12 (PF03522)

Summary: Solute carrier family 12

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This is the Wikipedia entry entitled "Electroneutral cation-Cl". More...

Electroneutral cation-Cl Edit Wikipedia article

K-Cl Co-transporter type 1 (KCC1)
Symbol KCl_Cotrans_1
Pfam PF03522
InterPro IPR018491
TCDB 2.A.30

In molecular biology, the electroneutral cation-Cl (electroneutral potassium chloride cotransporter) family of proteins are a family of solute carrier proteins. This family includes the products of the Human genes: SLC12A1, SLC12A1, SLC12A2, SLC12A3, SLC12A4, SLC12A5, SLC12A6, SLC12A7, SLC12A8 and SLC12A9.

The K-Cl co-transporter (KCC) mediates the coupled movement of K+ and Cl ions across the plasma membrane of many animal cells. This transport is involved in the regulatory volume decrease in response to cell swelling in red blood cells, and has been proposed to play a role in the vectorial movement of Cl across kidney epithelia. The transport process involves one for one electroneutral movement of K+ together with Cl, and, in all known mammalian cells, the net movement is outward.[1]

The neuronal KCC subtype KCC2 is cell-volume insensitive and plays a unique role in maintaining low intracellular Clconcentration, which is required in neurones for the functioning of Cl dependent fast synaptic inhibition, mediated by certain neurotransmitters, such as gamma-aminobutyric acid (GABA) and glycine.

Three isoforms of the K-Cl co-transporter have been described, termed KCC1 (SLC12A4), KCC2 (SLC12A5), and KCC3 (SLC12A6), containing 1085, 1116 and 1150 amino acids, respectively. They are predicted to have 12 transmembrane (TM) regions in a central hydrophobic domain, together with hydrophilic N- and C-termini that are likely cytoplasmic. Comparison of their sequences with those of other ion-transporting membrane proteins reveals that they are part of a new superfamily of cation-chloride co-transporters, which includes the Na-Cl and Na-K-2Cl co-transporters. KCC1 and KCC3 are widely expressed in human tissues, while KCC2 is expressed only in brain neurones, making it likely that this is the isoform responsible for maintaining low Cl concentration in neurones.[2][3][4]

KCC1 is widely expressed in human tissues, and when heterologously expressed, possesses the functional characteristics of the well-studied red blood cell K-Cl co-transporter, including stimulation by both swelling and N-ethylmaleimide. Several splice variants have also been identified.

KCC3 is widely expressed in human tissues and, like KCC1, is stimulated by both swelling and N-ethylmaleimide. The induction of KCC3 is up-regulated by vascular endothelial growth factor and down-regulated by tumour necrosis factor. Defects in KCC3 are linked to agenesis of the corpus callosum with peripheral neuropathy.[5] This disorder is characterised by severe progressive sensorimotor neuropathy, mental retardation, dysmorphic features and complete or partial agenesis of the corpus callosum.


  1. ^ Gillen CM, Brill S, Payne JA, Forbush B (July 1996). "Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human. A new member of the cation-chloride cotransporter family". J. Biol. Chem. 271 (27): 16237–44. doi:10.1074/jbc.271.27.16237. PMID 8663127. 
  2. ^ Payne JA, Stevenson TJ, Donaldson LF (July 1996). "Molecular characterization of a putative K-Cl cotransporter in rat brain. A neuronal-specific isoform". J. Biol. Chem. 271 (27): 16245–52. doi:10.1074/jbc.271.27.16245. PMID 8663311. 
  3. ^ Rivera C, Voipio J, Payne JA, Ruusuvuori E, Lahtinen H, Lamsa K, Pirvola U, Saarma M, Kaila K (January 1999). "The K+/Cl co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation". Nature. 397 (6716): 251–5. doi:10.1038/16697. PMID 9930699. 
  4. ^ Race JE, Makhlouf FN, Logue PJ, Wilson FH, Dunham PB, Holtzman EJ (December 1999). "Molecular cloning and functional characterization of KCC3, a new K-Cl cotransporter". Am. J. Physiol. 277 (6 Pt 1): C1210–9. PMID 10600773. 
  5. ^ Howard HC, Mount DB, Rochefort D, Byun N, Dupre N, Lu J, Fan X, Song L, Riviere JB, Prevost C, Horst J, Simonati A, Lemcke B, Welch R, England R, Zhan FQ, Mercado A, Siesser WB, George AL, McDonald MP, Bouchard JP, Mathieu J, Delpire E, Rouleau GA (November 2002). "The K-Cl cotransporter KCC3 is mutant in a severe peripheral neuropathy associated with agenesis of the corpus callosum". Nat. Genet. 32 (3): 384–92. doi:10.1038/ng1002. PMID 12368912. 

This article incorporates text from the public domain Pfam and InterPro IPR018491

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Solute carrier family 12 Provide feedback

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This tab holds annotation information from the InterPro database.

InterPro entry IPR018491

This entry represents a domain found in the C-terminal of the solute carrier family 12 (SLC12) members, which are K-Cl cotransporters. The SLC12 family members are predicted to have 12 transmembrane (TM) regions in a central hydrophobic domain, together with hydrophilic N- and C-termini that are likely cytoplasmic. Comparison of their sequences with those of other ion-tranporting membrane proteins reveals that they are part of a new superfamily of cation-chloride co-transporters, which includes the Na-Cl and Na-K-2Cl co-transporters [ PUBMED:8663311 , PUBMED:9930699 , PUBMED:10600773 ].

Gene Ontology

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Domain organisation

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Seed source: Jackhmmer:P55011
Previous IDs: KCl_Cotrans_1;
Type: Family
Sequence Ontology: SO:0100021
Author: Griffiths-Jones SR , Eberhardt R
Number in seed: 64
Number in full: 11971
Average length of the domain: 176.40 aa
Average identity of full alignment: 18 %
Average coverage of the sequence by the domain: 31.39 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.0
Noise cut-off 26.9 26.9
Model length: 417
Family (HMM) version: 17
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the SLC12 domain has been found. There are 31 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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