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13  structures 2692  species 0  interactions 6264  sequences 83  architectures

Family: SLAC1 (PF03595)

Summary: Voltage-dependent anion channel

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Voltage-dependent anion channel Provide feedback

This family of transporters has ten alpha helical transmembrane segments [1]. The structure of a bacterial homologue of SLAC1 shows it to have a trimeric arrangement. The pore is composed of five helices with a conserved Phe residue involved in gating. One homologue, Mae1 from the yeast Schizosaccharomyces pombe, functions as a malate uptake transporter; another, Ssu1 from Saccharomyces cerevisiae and other fungi including Aspergillus fumigatus, is characterised as a sulfite efflux pump; and TehA from Escherichia coli is identified as a tellurite resistance protein by virtue of its association in the tehA/tehB operon. In plants, this family is found in the stomatal guard cells functioning as an anion-transporting pore [2]. Many homologues are incorrectly annotated as tellurite resistance or dicarboxylate transporter (TDT) proteins.

Literature references

  1. Chen YH, Hu L, Punta M, Bruni R, Hillerich B, Kloss B, Rost B, Love J, Siegelbaum SA, Hendrickson WA;, Nature. 2010;467:1074-1080.: Homologue structure of the SLAC1 anion channel for closing stomata in leaves. PUBMED:20981093 EPMC:20981093

  2. Vahisalu T, Kollist H, Wang YF, Nishimura N, Chan WY, Valerio G, Lamminmaki A, Brosche M, Moldau H, Desikan R, Schroeder JI, Kangasjarvi J;, Nature. 2008;452:487-491.: SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling. PUBMED:18305484 EPMC:18305484


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR004695

Each of these transporters has ten alpha helical transmembrane segments [ PUBMED:20981093 ]. The structure of a bacterial homologue of SLAC1 shows it to have a trimeric arrangement. The pore is composed of five helices with a conserved Phe residue involved in gating. One homologue, Mae1 from the yeast Schizosaccharomyces pombe, functions as a malate uptake transporter; another, Ssu1from Saccharomyces cerevisiae and other fungi including Aspergillus fumigatus, is characterised as a sulfite efflux pump; and TehA from Escherichia coli is identified as a tellurite resistance protein by virtue of its association in the tehA/tehB operon. In plants, homologues are found in the stomatal guard cells functioning as an anion-transporting pore [ PUBMED:18305484 ]. Many homologues are incorrectly annotated as tellurite resistance or dicarboxylate transporter (TDT) proteins.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(432)
Full
(6264)
Representative proteomes UniProt
(19500)
RP15
(632)
RP35
(2575)
RP55
(5186)
RP75
(8798)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(432)
Full
(6264)
Representative proteomes UniProt
(19500)
RP15
(632)
RP35
(2575)
RP55
(5186)
RP75
(8798)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(432)
Full
(6264)
Representative proteomes UniProt
(19500)
RP15
(632)
RP35
(2575)
RP55
(5186)
RP75
(8798)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: TIGRFAMs
Previous IDs: C4dic_mal_tran;
Type: Family
Sequence Ontology: SO:0100021
Author: TIGRFAMs, Griffiths-Jones SR , Bateman A
Number in seed: 432
Number in full: 6264
Average length of the domain: 305.40 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 75.95 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.9 27.9
Trusted cut-off 28.0 27.9
Noise cut-off 27.8 27.8
Model length: 326
Family (HMM) version: 19
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the SLAC1 domain has been found. There are 13 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A096QUT4 View 3D Structure Click here
A0A0P0V174 View 3D Structure Click here
A0A0R0H3W5 View 3D Structure Click here
A0A0R0IDG7 View 3D Structure Click here
A0A0R0K2V3 View 3D Structure Click here
A0A1D6E265 View 3D Structure Click here
A0A1D6FL82 View 3D Structure Click here
A0A1D6FZR1 View 3D Structure Click here
A0A1D6MDW2 View 3D Structure Click here
A0A1D6MEP6 View 3D Structure Click here
A0A1D6MF67 View 3D Structure Click here
A0A1D6MXJ7 View 3D Structure Click here
A0A1D6NIB8 View 3D Structure Click here
A0A1D8PHX8 View 3D Structure Click here
A8MRV9 View 3D Structure Click here
B9EY47 View 3D Structure Click here
I1KNF9 View 3D Structure Click here
I1MPD0 View 3D Structure Click here
I1MPD1 View 3D Structure Click here
I1MR38 View 3D Structure Click here
K7KSQ4 View 3D Structure Click here
K7KYC4 View 3D Structure Click here
K7LDI5 View 3D Structure Click here
K7LKZ4 View 3D Structure Click here
K7UXS7 View 3D Structure Click here
K7V042 View 3D Structure Click here
O59815 View 3D Structure Click here
P0CT94 View 3D Structure Click here
P0CT95 View 3D Structure Click here
P0CT96 View 3D Structure Click here
P0CT97 View 3D Structure Click here
P25396 View 3D Structure Click here
P41930 View 3D Structure Click here
P50537 View 3D Structure Click here
P9WLS5 View 3D Structure Click here
Q2FZA6 View 3D Structure Click here
Q54TN7 View 3D Structure Click here
Q57996 View 3D Structure Click here
Q58172 View 3D Structure Click here
Q5A3Z6 View 3D Structure Click here
Q5E930 View 3D Structure Click here
Q5NAK4 View 3D Structure Click here
Q5W6C1 View 3D Structure Click here
Q6ZLB6 View 3D Structure Click here
Q75G55 View 3D Structure Click here
Q7XTX7 View 3D Structure Click here
Q8L4G8 View 3D Structure Click here
Q9ASQ7 View 3D Structure Click here
Q9FLV9 View 3D Structure Click here
Q9LD83 View 3D Structure Click here