Summary: Arginine-tRNA-protein transferase, C terminus
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Arginine-tRNA-protein transferase, C terminus Provide feedback
This family represents the C terminal region of the enzyme arginine-tRNA-protein transferase (EC 2.3.2.8), which catalyses the post-translational conjugation of arginine to the N terminus of a protein. In eukaryotes, this functions as part of the N-end rule pathway of protein degradation by conjugating a destabilising amino acid to the amino terminal aspartate or glutamate of a protein, targeting the protein for ubiquitin-dependent proteolysis. N terminal cysteine is sometimes modified [1].
Literature references
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Kwon YT, Kashina AS, Varshavsky A; , Mol Cell Biol 1999;19:182-193.: Alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-tRNA-protein transferase, a component of the N-end rule pathway. PUBMED:9858543 EPMC:9858543
Internal database links
SCOOP: | Acetyltransf_6 |
Similarity to PfamA using HHSearch: | Acetyltransf_6 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR007472
This entry represents the C-terminal region of aminoacyl-transferases found in both eukaryotic (Arginine-tRNA-protein transferase) and prokaryotic (Aspartate/glutamate leucyltransferase) enzymes.
Arginine-tRNA-protein transferase catalyses the post-translational conjugation of arginine to the N terminus of a protein. In eukaryotes, this functions as part of the N-end rule pathway of protein degradation by conjugating a destabilising amino acid to the N-terminal aspartate or glutamate of a protein, targeting the protein for ubiquitin-dependent proteolysis [PUBMED:9858543]. In Saccharomyces cerevisiae, Cys20, 23, 94 and/or 95 are thought to be important for activity [PUBMED:7495814]. Of these, only Cys 94 appears to be completely conserved in this family.
Aspartate/glutamate leucyltransferase (also known as bacterial protein transferase or Bpt) functions in the N-end rule pathway of protein degradation where it conjugates Leu from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate. This protein shows sequence similarity to the eukaryotic N-end rule pathway component arginyl-transferase Ate1 [PUBMED:16492767].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | arginyltransferase activity (GO:0004057) |
Biological process | protein arginylation (GO:0016598) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan Acetyltrans (CL0257), which has the following description:
This clan contains families related to N-acetyltransferases. N-acetyltransferases catalyse the transfer of acetyl groups from acetyl-CoA to arylamines.
The clan contains the following 45 members:
Acetyltransf_1 Acetyltransf_10 Acetyltransf_13 Acetyltransf_15 Acetyltransf_16 Acetyltransf_17 Acetyltransf_18 Acetyltransf_19 Acetyltransf_3 Acetyltransf_4 Acetyltransf_5 Acetyltransf_6 Acetyltransf_7 Acetyltransf_8 Acetyltransf_9 Acetyltransf_CG AstA ATE_C ATE_N Autoind_synth DUF1122 DUF1248 DUF1999 DUF2156 DUF5613 DUF5645 FemAB FemAB_like FR47 Gly_acyl_tr_C GNAT_acetyltr_2 GNAT_acetyltran GNAT_C HAT_KAT11 HlyC Leu_Phe_trans MCD Mig-14 MOZ_SAS NAT NMT NMT_C NodA ODC_AZ PanZAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (340) |
Full (3843) |
Representative proteomes | UniProt (12771) |
NCBI (15909) |
Meta (378) |
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RP15 (526) |
RP35 (1661) |
RP55 (3331) |
RP75 (5603) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (340) |
Full (3843) |
Representative proteomes | UniProt (12771) |
NCBI (15909) |
Meta (378) |
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RP15 (526) |
RP35 (1661) |
RP55 (3331) |
RP75 (5603) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
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Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | COG2935 |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Kerrison ND |
Number in seed: | 340 |
Number in full: | 3843 |
Average length of the domain: | 130.10 aa |
Average identity of full alignment: | 35 % |
Average coverage of the sequence by the domain: | 37.41 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 134 | ||||||||||||
Family (HMM) version: | 16 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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