Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
60  structures 1340  species 0  interactions 8648  sequences 160  architectures

Family: Anoctamin (PF04547)

Summary: Calcium-activated chloride channel

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Calcium-activated chloride channel Provide feedback

The family carries eight putative transmembrane domains, and, although it has no similarity to other known channel proteins, it is clearly a calcium-activated ionic channel. It is expressed in various secretory epithelia, the retina and sensory neurons, and mediates receptor-activated chloride currents in diverse physiological processes [1].

Literature references

  1. Yang YD, Cho H, Koo JY, Tak MH, Cho Y, Shim WS, Park SP, Lee J, Lee B, Kim BM, Raouf R, Shin YK, Oh U; , Nature, 2008; [Epub ahead of print]: TMEM16A confers receptor-activated calcium-dependent chloride conductance. PUBMED:18724360 EPMC:18724360


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007632

This entry represents the anoctamin family, which includes anoctamin1-10 (Ano1-10 or TMEM16A-J); 7 members could be divided into two subfamilies, Ca(2+)-dependent Cl(-) channels (TMEM16A and 16B) and Ca(2+)-dependent lipid scramblases (TMEM16C, 16D, 16F, 16G, and 16J) [ PUBMED:23532839 ]. This entry also includes anoctamin-like protein At1g73020 from Arabidopsis and increased sodium tolerance protein 2 (Ist2) from budding yeasts.

Ano1 and Ano2 (also known as TMEM16A and TMEM16B) are calcium-activated chloride channels (CaCC), which play a role in transepithelial anion transport and smooth muscle contraction [ PUBMED:18724360 , PUBMED:19474308 ]. Ano3-10 do not exhibit calcium-activated chloride channel (CaCC) activity [ PUBMED:17308099 , PUBMED:20056604 ].

Ist2 may be involved in ion homeostasis together with BTN1 or BTN2 [ PUBMED:15701790 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan Anoctamin-like (CL0416), which has the following description:

The families are transmembrane families, where some of the members have sodium tolerance activity.

The clan contains the following 3 members:

Anoctamin RSN1_7TM TMC

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(315)
Full
(8648)
Representative proteomes UniProt
(17333)
RP15
(1567)
RP35
(3590)
RP55
(7278)
RP75
(9559)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(315)
Full
(8648)
Representative proteomes UniProt
(17333)
RP15
(1567)
RP35
(3590)
RP55
(7278)
RP75
(9559)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(315)
Full
(8648)
Representative proteomes UniProt
(17333)
RP15
(1567)
RP35
(3590)
RP55
(7278)
RP75
(9559)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_2735 (release 7.5)
Previous IDs: DUF590;
Type: Family
Sequence Ontology: SO:0100021
Author: Mifsud W
Number in seed: 315
Number in full: 8648
Average length of the domain: 457.80 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 60.16 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.0 28.0
Trusted cut-off 28.1 28.0
Noise cut-off 27.7 27.9
Model length: 450
Family (HMM) version: 14
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Anoctamin domain has been found. There are 60 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0B4JCY1 View 3D Structure Click here
A0A0G2JXB5 View 3D Structure Click here
A0A0G2K0I0 View 3D Structure Click here
A0A0G2K1M7 View 3D Structure Click here
A0A0G2K5K1 View 3D Structure Click here
A0A0G2L2L3 View 3D Structure Click here
A0A0R0FXK3 View 3D Structure Click here
A0A0R0IAR9 View 3D Structure Click here
A0A0R4IR43 View 3D Structure Click here
A0A1D6FKW1 View 3D Structure Click here
A0A1D6G2Z4 View 3D Structure Click here
A0A1D6NEF8 View 3D Structure Click here
A0A1D6QCR3 View 3D Structure Click here
A0A1D8PE41 View 3D Structure Click here
A0A2R8Q7A1 View 3D Structure Click here
A0A2R8Q9Q7 View 3D Structure Click here
A0A2R8QGR3 View 3D Structure Click here
A0A2R8QM29 View 3D Structure Click here
A0A2R8RJ22 View 3D Structure Click here
A0MFS9 View 3D Structure Click here
A1A5B4 View 3D Structure Click here
A2AHL1 View 3D Structure Click here
A4HVK2 View 3D Structure Click here
D3ZF54 View 3D Structure Click here
D4A915 View 3D Structure Click here
E7F191 View 3D Structure Click here
E7F3A3 View 3D Structure Click here
E7F4N6 View 3D Structure Click here
E7F503 View 3D Structure Click here
E7FD02 View 3D Structure Click here
F1LY14 View 3D Structure Click here
F1LZ77 View 3D Structure Click here
F1M0A0 View 3D Structure Click here
F1QC71 View 3D Structure Click here
F1QNZ8 View 3D Structure Click here
F1QVG0 View 3D Structure Click here
G5EBW3 View 3D Structure Click here
H2L2L3 View 3D Structure Click here
I1KGF8 View 3D Structure Click here
I1KUY0 View 3D Structure Click here
K7MFZ9 View 3D Structure Click here
O13621 View 3D Structure Click here
O97132 View 3D Structure Click here
P38250 View 3D Structure Click here
P86044 View 3D Structure Click here
Q0JJZ6 View 3D Structure Click here
Q14AT5 View 3D Structure Click here
Q32M45 View 3D Structure Click here
Q4KMQ2 View 3D Structure Click here
Q4V8U5 View 3D Structure Click here
Q54BH1 View 3D Structure Click here
Q55GA1 View 3D Structure Click here
Q5XXA6 View 3D Structure Click here
Q6IFT6 View 3D Structure Click here
Q6IWH7 View 3D Structure Click here
Q6P9J9 View 3D Structure Click here
Q6PB70 View 3D Structure Click here
Q75UR0 View 3D Structure Click here
Q75V66 View 3D Structure Click here
Q76NS2 View 3D Structure Click here
Q8BH79 View 3D Structure Click here
Q8BHY3 View 3D Structure Click here
Q8C5H1 View 3D Structure Click here
Q8CFW1 View 3D Structure Click here
Q9BYT9 View 3D Structure Click here
Q9HCE9 View 3D Structure Click here
Q9NQ90 View 3D Structure Click here
Q9NW15 View 3D Structure Click here
Q9V416 View 3D Structure Click here
X1WC49 View 3D Structure Click here