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110  structures 1083  species 0  interactions 2349  sequences 37  architectures

Family: MCU (PF04678)

Summary: Mitochondrial calcium uniporter

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mitochondrial calcium uniporter Provide feedback

MCU functions with MICU1, an essential gatekeeper component of calcium-channel transport, to facilitate Ca2+ uptake into the mitochondrion [1,2,3].

Literature references

  1. De Stefani D, Raffaello A, Teardo E, Szabo I, Rizzuto R;, Nature. 2011;476:336-340.: A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. PUBMED:21685888 EPMC:21685888

  2. Drago I, Pizzo P, Pozzan T;, EMBO J. 2011;30:4119-4125.: After half a century mitochondrial calcium in- and efflux machineries reveal themselves. PUBMED:21934651 EPMC:21934651

  3. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK;, Science. 2013;342:1379-1382.: EMRE is an essential component of the mitochondrial calcium uniporter complex. PUBMED:24231807 EPMC:24231807

  4. Mallilankaraman K, Cardenas C, Doonan PJ, Chandramoorthy HC, Irrinki KM, Golenar T, Csordas G, Madireddi P, Yang J, Muller M, Miller R, Kolesar JE, Molgo J, Kaufman B, Hajnoczky G, Foskett JK, Madesh M;, Nat Cell Biol. 2012;14:1336-1343: MCUR1 is an essential component of mitochondrial Ca2+ uptake that regulates cellular metabolism. PUBMED:23178883 EPMC:23178883


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006769

This entry represents the C-terminal domain of MCU, which is a mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria [ PUBMED:21685888 , PUBMED:21685886 , PUBMED:23101630 ]. This domain can also be found in MCUb, which negatively regulates the activity of MCU [ PUBMED:23900286 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(86)
Full
(2349)
Representative proteomes UniProt
(3825)
RP15
(376)
RP35
(1097)
RP55
(1902)
RP75
(2560)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(86)
Full
(2349)
Representative proteomes UniProt
(3825)
RP15
(376)
RP35
(1097)
RP55
(1902)
RP75
(2560)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(86)
Full
(2349)
Representative proteomes UniProt
(3825)
RP15
(376)
RP35
(1097)
RP55
(1902)
RP75
(2560)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_5620 (release 7.5)
Previous IDs: DUF607;
Type: Family
Sequence Ontology: SO:0100021
Author: Mifsud W
Number in seed: 86
Number in full: 2349
Average length of the domain: 169.00 aa
Average identity of full alignment: 30 %
Average coverage of the sequence by the domain: 47.50 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 30.8 30.8
Trusted cut-off 31.0 30.8
Noise cut-off 30.5 30.6
Model length: 177
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MCU domain has been found. There are 110 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0R0FI60 View 3D Structure Click here
A0A0R0FQK7 View 3D Structure Click here
A0A0R0HDM2 View 3D Structure Click here
A0A1D6JU72 View 3D Structure Click here
A0A1D6KRF1 View 3D Structure Click here
A0A1D6NQL8 View 3D Structure Click here
A4HVI9 View 3D Structure Click here
A4HZ34 View 3D Structure Click here
A4HZH5 View 3D Structure Click here
B9G951 View 3D Structure Click here
C0HJ12 View 3D Structure Click here
C4IZV8 View 3D Structure Click here
E9AHF0 View 3D Structure Click here
F1LUQ0 View 3D Structure Click here
F4I111 View 3D Structure Click here
I1J8Z1 View 3D Structure Click here
I1JCS9 View 3D Structure Click here
I1JHP5 View 3D Structure Click here
I1MBN2 View 3D Structure Click here
I1MXU4 View 3D Structure Click here
K7LR85 View 3D Structure Click here
K7M5H4 View 3D Structure Click here
K7MQ00 View 3D Structure Click here
K7W1M1 View 3D Structure Click here
M0RDI5 View 3D Structure Click here
O64823 View 3D Structure Click here
Q08BI9 View 3D Structure Click here
Q1PE15 View 3D Structure Click here
Q21121 View 3D Structure Click here
Q2QYF2 View 3D Structure Click here
Q3UMR5 View 3D Structure Click here
Q4CKY9 View 3D Structure Click here
Q4CZP7 View 3D Structure Click here
Q4DAW7 View 3D Structure Click here
Q4DHJ5 View 3D Structure Click here
Q4DHT1 View 3D Structure Click here
Q4DL37 View 3D Structure Click here
Q4DYG3 View 3D Structure Click here
Q54LT0 View 3D Structure Click here
Q5QML5 View 3D Structure Click here
Q810S1 View 3D Structure Click here
Q8IQ70 View 3D Structure Click here
Q8LNF6 View 3D Structure Click here
Q8NE86 View 3D Structure Click here
Q8S5X6 View 3D Structure Click here
Q8VYR0 View 3D Structure Click here
Q9FJV7 View 3D Structure Click here
Q9LVR5 View 3D Structure Click here
Q9NWR8 View 3D Structure Click here