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56  structures 1532  species 0  interactions 3850  sequences 85  architectures

Family: Evr1_Alr (PF04777)

Summary: Erv1 / Alr family

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Erv1 / Alr family Provide feedback

Biogenesis of Fe/S clusters involves a number of essential mitochondrial proteins. Erv1p of Saccharomyces cerevisiae mitochondria is required for the maturation of Fe/S proteins in the cytosol. The ALR (augmenter of liver regeneration) represents a mammalian orthologue of yeast Erv1p. Both Erv1p and full-length ALR are located in the mitochondrial intermembrane an d it thought to operate downstream of the mitochondrial ABC transporter [1].

Literature references

  1. Lange H, Lisowsky T, Gerber J, Muhlenhoff U, Kispal G, Lill R; , EMBO Rep 2001;2:715-720.: An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins. PUBMED:11493598 EPMC:11493598


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR017905

The ~100-residue ERV/ALR sulphydryl oxidase domain is a versatile module adapted for catalysis of disulphide bond formation in various organelles and biological settings. The ERV/ALR sulphydryl oxidase domain has a Cys-X-X-Cys dithiol/disulphide motif adjacent to a bound FAD cofactor, enabling transfer of electrons from thiol substrates to non-thiol electron acceptors. ERV/ALR family members differ in their N- or C-terminal extensions, which typically contain at least one additional disulphide bond, the hypothesised 'shuttle' disulphide. In yeast ERV1, a mitochondrial enzyme, the shuttle disulphide is N-terminal to the catalytic core; in yeast ERV2, present in the endoplasmic reticulum, it is C-terminal. The N- and C-terminal extensions can be entire domains, such as the thioredoxin-like domains ( PROSITEDOC ) or short segments that do not seem to be distinct domains. Proteins of the ERV/ALR family are encoded by all eukaryotes and cytoplasmic DNA viruses (poxviruses, African swine fever virus, iridoviruses, and Paramecium bursaria Chlorella virus 1) [ PUBMED:10542195 , PUBMED:11035794 , PUBMED:11740506 , PUBMED:16893552 , PUBMED:17298084 ].

The ERV/ALR sulphydryl oxidase domain contains a four-helix bundle (helices alpha1-alpha4) and an additional single turn of helix (alpha5) packed perpendicular to the bundle [ PUBMED:1174050 , PUBMED:16893552 ]. The FAD prosthetic group is housed at the mouth of the 4-helix bundle and communicates with the pair of juxtaposed cysteine residues that form the proximal redox active site [ PUBMED:17298084 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(301)
Full
(3850)
Representative proteomes UniProt
(8163)
RP15
(915)
RP35
(1930)
RP55
(3101)
RP75
(4079)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(301)
Full
(3850)
Representative proteomes UniProt
(8163)
RP15
(915)
RP35
(1930)
RP55
(3101)
RP75
(4079)
Alignment:
Format:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(301)
Full
(3850)
Representative proteomes UniProt
(8163)
RP15
(915)
RP35
(1930)
RP55
(3101)
RP75
(4079)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_5005 (release 7.6)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD
Number in seed: 301
Number in full: 3850
Average length of the domain: 94.90 aa
Average identity of full alignment: 31 %
Average coverage of the sequence by the domain: 26.73 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.6 27.6
Trusted cut-off 27.6 27.6
Noise cut-off 27.5 27.5
Model length: 95
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Evr1_Alr domain has been found. There are 56 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2KAE4 View 3D Structure Click here
A0A1D6JRN1 View 3D Structure Click here
A0A1D6MBW0 View 3D Structure Click here
A0A1D8PSD6 View 3D Structure Click here
A4HWJ4 View 3D Structure Click here
A4I571 View 3D Structure Click here
A4QNU9 View 3D Structure Click here
B0UXN0 View 3D Structure Click here
C0PVB3 View 3D Structure Click here
D3ZP13 View 3D Structure Click here
F1QJL3 View 3D Structure Click here
H2KZY5 View 3D Structure Click here
I1LEN2 View 3D Structure Click here
I1M0U8 View 3D Structure Click here
I1MIT7 View 3D Structure Click here
I1NFT7 View 3D Structure Click here
O00391 View 3D Structure Click here
O14144 View 3D Structure Click here
O44975 View 3D Structure Click here
P27882 View 3D Structure Click here
P55789 View 3D Structure Click here
P56213 View 3D Structure Click here
P91442 View 3D Structure Click here
Q10Q80 View 3D Structure Click here
Q12284 View 3D Structure Click here
Q20063 View 3D Structure Click here
Q3TMX7 View 3D Structure Click here
Q4CM05 View 3D Structure Click here
Q4CUK9 View 3D Structure Click here
Q4CVG9 View 3D Structure Click here
Q4CWP6 View 3D Structure Click here
Q4DHN4 View 3D Structure Click here
Q5AGY6 View 3D Structure Click here
Q63042 View 3D Structure Click here
Q6AUC6 View 3D Structure Click here
Q6IUU3 View 3D Structure Click here
Q6ZRP7 View 3D Structure Click here
Q75JQ5 View 3D Structure Click here
Q7JQR3 View 3D Structure Click here
Q8BND5 View 3D Structure Click here
Q8GXX0 View 3D Structure Click here
Q8I242 View 3D Structure Click here
Q8I509 View 3D Structure Click here
Q8IMY4 View 3D Structure Click here
Q8W4J3 View 3D Structure Click here
Q9VD61 View 3D Structure Click here
Q9VD62 View 3D Structure Click here
Q9Y806 View 3D Structure Click here
Q9ZU40 View 3D Structure Click here