Summary: ALG3 protein
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ALG3 protein Provide feedback
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man [3]. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
Literature references
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Korner C, Knauer R, Stephani U, Marquardt T, Lehle L, von Figura K; , EMBO J 1999;18:6816-6822.: Carbohydrate deficient glycoprotein syndrome type IV: deficiency of dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase. PUBMED:10581255 EPMC:10581255
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Aebi M, Gassenhuber J, Domdey H, te Heesen S; , Glycobiology 1996;6:439-444.: Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae. PUBMED:8842708 EPMC:8842708
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Sharma CB, Knauer R, Lehle L; , Biol Chem 2001;382:321-328.: Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase. PUBMED:11308030 EPMC:11308030
Internal database links
SCOOP: | EpsG GT87 |
External database links
CAZY: | GT58 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR007873
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man [PUBMED:11308030]. The ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | integral component of endoplasmic reticulum membrane (GO:0030176) |
Molecular function | mannosyltransferase activity (GO:0000030) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan GT-C (CL0111), which has the following description:
This is the GT-C clan that contains diverse glycosyltransferases that possess 8-13 predicted transmembrane segments [1].
The clan contains the following 19 members:
ALG3 Alg6_Alg8 Arabinose_trans DIE2_ALG10 DUF2079 DUF2142 DUF2723 EpsG Glucan_synthase Glyco_transf_22 GT87 Mannosyl_trans Mannosyl_trans2 PIG-U PMT PMT_2 PTPS_related STT3 YfhOAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (143) |
Full (1412) |
Representative proteomes | UniProt (2491) |
NCBI (3160) |
Meta (15) |
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RP15 (237) |
RP35 (641) |
RP55 (1026) |
RP75 (1422) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (143) |
Full (1412) |
Representative proteomes | UniProt (2491) |
NCBI (3160) |
Meta (15) |
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RP15 (237) |
RP35 (641) |
RP55 (1026) |
RP75 (1422) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_3416 (release 7.7) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Wood V |
Number in seed: | 143 |
Number in full: | 1412 |
Average length of the domain: | 317.20 aa |
Average identity of full alignment: | 42 % |
Average coverage of the sequence by the domain: | 77.89 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 358 | ||||||||||||
Family (HMM) version: | 14 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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