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10  structures 3752  species 0  interactions 6503  sequences 117  architectures

Family: GGACT (PF06094)

Summary: Gamma-glutamyl cyclotransferase, AIG2-like

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Gamma-glutamyl cyclotransferase, AIG2-like Provide feedback

GGACT, gamma-glutamylamine cyclotransferase, is a ubiquitous enzyme found in bacteria, plants, and metazoans from Dictyostelium through to humans. It converts gamma-glutamylamines to free amines and 5-oxoproline.

Literature references

  1. Reuber TL, Ausubel FM; , Plant Cell 1996;8:241-249.: Isolation of Arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes. PUBMED:8742710 EPMC:8742710

  2. Lytle BL, Peterson FC, Tyler EM, Newman CL, Vinarov DA, Markley JL, Volkman BF; , Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006;62:490-493.: Solution structure of Arabidopsis thaliana protein At5g39720.1, a member of the AIG2-like protein family. PUBMED:16754964 EPMC:16754964

  3. Jensen MH, Otten H, Christensen U, Borchert TV, Christensen LL, Larsen S, Leggio LL;, J Mol Biol. 2010;404:100-111.: Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus. PUBMED:20851126 EPMC:20851126

  4. Oakley AJ, Coggan M, Board PG;, J Biol Chem. 2010;285:9642-9648.: Identification and characterization of gamma-glutamylamine cyclotransferase, an enzyme responsible for gamma-glutamyl-epsilon-lysine catabolism. PUBMED:20110353 EPMC:20110353

  5. Llewellyn NM, Li Y, Spencer JB;, Chem Biol. 2007;14:379-386.: Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain. PUBMED:17462573 EPMC:17462573


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR009288

This entry represents a domain found in a group of gamma-glutamyl cyclotransferases (GGCTs), including AIG2 from Arabidopsis. GGCT is a ubiquitous enzyme found in bacteria, plants, and metazoans from Dictyostelium through to humans. It converts gamma-glutamylamines to free amines and 5-oxoproline [ PUBMED:20110353 , PUBMED:20851126 , PUBMED:17462573 ].

AIG2 is an Arabidopsis protein that exhibit RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2 [ PUBMED:8742710 ]. Its structure consists of a five-stranded beta-barrel surrounded by two alpha-helices and a small beta-sheet. A long flexible alpha-helix protrudes from the structure at the C-terminal end. Conserved residues in a hydrophilic cavity, which are able to bind small ligands, may act as an active site in AIG2-like proteins [ PUBMED:16754964 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan AIG2 (CL0278), which has the following description:

The structure consists of a five-stranded beta-barrel surrounded by two alpha-helices and a small beta-sheet. Conservation of residues in a hydrophilic cavity able to bind small ligands in some members suggests that this may also serve as an active site.

The clan contains the following 3 members:

AIG2_2 ChaC GGACT

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(325)
Full
(6503)
Representative proteomes UniProt
(20863)
RP15
(908)
RP35
(2968)
RP55
(5740)
RP75
(9390)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(325)
Full
(6503)
Representative proteomes UniProt
(20863)
RP15
(908)
RP35
(2968)
RP55
(5740)
RP75
(9390)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(325)
Full
(6503)
Representative proteomes UniProt
(20863)
RP15
(908)
RP35
(2968)
RP55
(5740)
RP75
(9390)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_9771 (release 9.0)
Previous IDs: AIG2;
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD
Number in seed: 325
Number in full: 6503
Average length of the domain: 123.30 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 61.79 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.8 22.8
Trusted cut-off 22.8 22.8
Noise cut-off 22.7 22.7
Model length: 122
Family (HMM) version: 14
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the GGACT domain has been found. There are 10 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0XRK7 View 3D Structure Click here
A0A0P0XZ44 View 3D Structure Click here
A0A1D6H0C6 View 3D Structure Click here
A0A1D6IBA5 View 3D Structure Click here
A0JMM9 View 3D Structure Click here
A2RVS4 View 3D Structure Click here
A3KNL6 View 3D Structure Click here
A4I3V2 View 3D Structure Click here
A8MRP2 View 3D Structure Click here
B4FVZ7 View 3D Structure Click here
C0PD93 View 3D Structure Click here
C6T4K7 View 3D Structure Click here
C6TFS9 View 3D Structure Click here
I1J4V3 View 3D Structure Click here
I1LJ44 View 3D Structure Click here
I1LP52 View 3D Structure Click here
P0AE48 View 3D Structure Click here
P54121 View 3D Structure Click here
Q4CQV4 View 3D Structure Click here
Q4D422 View 3D Structure Click here
Q4KM86 View 3D Structure Click here
Q54WQ4 View 3D Structure Click here
Q57876 View 3D Structure Click here
Q58909 View 3D Structure Click here
Q5Z825 View 3D Structure Click here
Q65ZK1 View 3D Structure Click here
Q66I06 View 3D Structure Click here
Q6Z2W4 View 3D Structure Click here
Q84T74 View 3D Structure Click here
Q923B0 View 3D Structure Click here
Q9BVM4 View 3D Structure Click here
Q9FIQ3 View 3D Structure Click here
Q9FIX1 View 3D Structure Click here
Q9FIX2 View 3D Structure Click here
Q9M8T3 View 3D Structure Click here
Q9MBH1 View 3D Structure Click here
Q9MBH2 View 3D Structure Click here
Q9W0Y1 View 3D Structure Click here
Q9W0Y2 View 3D Structure Click here