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16  structures 1355  species 0  interactions 8808  sequences 129  architectures

Family: ANTH (PF07651)

Summary: ANTH domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "ANTH domain". More...

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

ANTH domain Provide feedback

AP180 is an endocytotic accessory proteins that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats [1,2].

Literature references

  1. Stahelin RV, Long F, Peter BJ, Murray D, De Camilli P, McMahon HT, Cho W; , J Biol Chem 2003;278:28993-28999.: Contrasting membrane interaction mechanisms of AP180 N-terminal homology (ANTH) and epsin N-terminal homology (ENTH) domains. PUBMED:12740367 EPMC:12740367

  2. Duncan MC, Payne GS; , Trends Cell Biol 2003;13:211-215.: ENTH/ANTH domains expand to the Golgi. PUBMED:12742163 EPMC:12742163


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR011417

The AP180 N-terminal homology (ANTH) domain is a membrane binding domain found in endocytotic accessory proteins, such as AP180. AP180 has been implicated in the formation of clathrin-coated pits. The ANTH domain is involved in phosphatidylinositol 4,5-bisphosphate (also known as PIP2) binding. The ANTH domain containing proteins appear to be universal elements in nucleation of clathrin coats [ PUBMED:12740367 , PUBMED:12742163 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan ENTH_VHS (CL0009), which has the following description:

This clan includes the related ENTH and ANTH domains as well as the VHS domain. The ENTH domain is approximately 150 residues in length and is a solenoid of alpha-helices. The various ENTH domains have various lipid specificities but the key feature that distinguishes it functionally from ANTH domains is its ability to bend membranes. It does this by folding an additional N-terminal helix on lipid binding. The ANTH domain is approximately 300 residues in length and is a PtdIns(4,5)P2 binding domain. It has no membrane bending properties. The VHS (Vps-27, Hrs and STAM) domain is a 140 residue long domain present in the very NH2-terminus of at least 60 proteins. Based on their functional characteristics and on recent data on the involvement of VHS in cargo recognition in trans-Golgi, VHS domains are considered to have a general membrane targeting/cargo recognition role in vesicular trafficking [5].

The clan contains the following 3 members:

ANTH ENTH VHS

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(28)
Full
(8808)
Representative proteomes UniProt
(14837)
RP15
(1048)
RP35
(3824)
RP55
(7409)
RP75
(10141)
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HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(28)
Full
(8808)
Representative proteomes UniProt
(14837)
RP15
(1048)
RP35
(3824)
RP55
(7409)
RP75
(10141)
Alignment:
Format:
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Sequence:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(28)
Full
(8808)
Representative proteomes UniProt
(14837)
RP15
(1048)
RP35
(3824)
RP55
(7409)
RP75
(10141)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: [1]
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Finn RD , Bateman A , McMahon HT
Number in seed: 28
Number in full: 8808
Average length of the domain: 236.90 aa
Average identity of full alignment: 26 %
Average coverage of the sequence by the domain: 37.34 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.5 21.5
Trusted cut-off 21.5 21.5
Noise cut-off 21.4 21.4
Model length: 277
Family (HMM) version: 18
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ANTH domain has been found. There are 16 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0VCQ0 View 3D Structure Click here
A0A0P0WZI5 View 3D Structure Click here
A0A0P0X0S0 View 3D Structure Click here
A0A0P0XNR6 View 3D Structure Click here
A0A0R4IKR8 View 3D Structure Click here
A0A0R4IZC2 View 3D Structure Click here
A0A1D6F560 View 3D Structure Click here
A0A1D6FJI7 View 3D Structure Click here
A0A1D6FJI7 View 3D Structure Click here
A0A1D6KCF1 View 3D Structure Click here
A0A1D6KCF1 View 3D Structure Click here
A0A1D6KL69 View 3D Structure Click here
A0A1D6LQE1 View 3D Structure Click here
A0A1D6LY69 View 3D Structure Click here
A0A1D6NES1 View 3D Structure Click here
A0A1D6NU34 View 3D Structure Click here
A0A1D6P0Y2 View 3D Structure Click here
A0A1D6P0Y2 View 3D Structure Click here
A0A1D6PE57 View 3D Structure Click here
A0A1D6PKM6 View 3D Structure Click here
A0A1D6QBX5 View 3D Structure Click here
A0A1D8PL36 View 3D Structure Click here
A0A1D8PRF5 View 3D Structure Click here
A0A286Y9N9 View 3D Structure Click here
A0A286Y9Z9 View 3D Structure Click here
A0A286YAZ9 View 3D Structure Click here
A0A2R8Q859 View 3D Structure Click here
A0A2R8QB02 View 3D Structure Click here
A0A2R8QHW2 View 3D Structure Click here
A4I0M5 View 3D Structure Click here
B4FLN4 View 3D Structure Click here
B6SKK6 View 3D Structure Click here
B6TYB1 View 3D Structure Click here
B6TYB1 View 3D Structure Click here
B7ZYW2 View 3D Structure Click here
C0P489 View 3D Structure Click here
C0P595 View 3D Structure Click here
C0PJN1 View 3D Structure Click here
E7FD55 View 3D Structure Click here
F1LML7 View 3D Structure Click here
G3V8Y8 View 3D Structure Click here
I1JUC2 View 3D Structure Click here
I1JUC2 View 3D Structure Click here
I1JVA2 View 3D Structure Click here
I1K2S4 View 3D Structure Click here
I1K3B5 View 3D Structure Click here
I1K3D9 View 3D Structure Click here
I1K4G2 View 3D Structure Click here
I1K5H4 View 3D Structure Click here
I1K8U2 View 3D Structure Click here
I1K9U2 View 3D Structure Click here
I1KRP6 View 3D Structure Click here
I1KRS8 View 3D Structure Click here
I1KUF9 View 3D Structure Click here
I1L1D8 View 3D Structure Click here
I1L3M6 View 3D Structure Click here
I1LNH0 View 3D Structure Click here
I1LVM2 View 3D Structure Click here
I1LZP6 View 3D Structure Click here
I1LZR5 View 3D Structure Click here
I1MAX6 View 3D Structure Click here
I1MDU2 View 3D Structure Click here
I1MH35 View 3D Structure Click here
I1MQA9 View 3D Structure Click here
I1MSL6 View 3D Structure Click here
I1MU50 View 3D Structure Click here
I1MYN3 View 3D Structure Click here
I1ND41 View 3D Structure Click here
K7KWL8 View 3D Structure Click here
K7L2G1 View 3D Structure Click here
K7L503 View 3D Structure Click here
K7L6F9 View 3D Structure Click here
K7LDE5 View 3D Structure Click here
K7LYR1 View 3D Structure Click here
K7MNL2 View 3D Structure Click here
K7UQ10 View 3D Structure Click here
K7V3D3 View 3D Structure Click here
K7VMV9 View 3D Structure Click here
O00291 View 3D Structure Click here
O55012 View 3D Structure Click here
O60167 View 3D Structure Click here
O60641 View 3D Structure Click here
O62142 View 3D Structure Click here
O75146 View 3D Structure Click here
P33338 View 3D Structure Click here
P38856 View 3D Structure Click here
P53309 View 3D Structure Click here
P94017 View 3D Structure Click here
Q02328 View 3D Structure Click here
Q05140 View 3D Structure Click here
Q0DG77 View 3D Structure Click here
Q0ITF5 View 3D Structure Click here
Q10NB4 View 3D Structure Click here
Q13492 View 3D Structure Click here
Q1ZXE9 View 3D Structure Click here
Q4CRX2 View 3D Structure Click here
Q4E0C4 View 3D Structure Click here
Q54VV7 View 3D Structure Click here
Q54Y50 View 3D Structure Click here
Q5N9P9 View 3D Structure Click here
Q60EC1 View 3D Structure Click here
Q61548 View 3D Structure Click here
Q658F5 View 3D Structure Click here
Q65XV1 View 3D Structure Click here
Q69SJ3 View 3D Structure Click here
Q6EUQ6 View 3D Structure Click here
Q6YS99 View 3D Structure Click here
Q6YS99 View 3D Structure Click here
Q7M6Y3 View 3D Structure Click here
Q8GX47 View 3D Structure Click here
Q8H0W9 View 3D Structure Click here
Q8L936 View 3D Structure Click here
Q8LBH2 View 3D Structure Click here
Q8LF20 View 3D Structure Click here
Q8LF20 View 3D Structure Click here
Q8LJJ6 View 3D Structure Click here
Q8MQJ8 View 3D Structure Click here
Q8S9J8 View 3D Structure Click here
Q8VD75 View 3D Structure Click here
Q8VYT2 View 3D Structure Click here
Q9C502 View 3D Structure Click here
Q9C9X5 View 3D Structure Click here
Q9FKQ2 View 3D Structure Click here
Q9FRH3 View 3D Structure Click here
Q9JKY5 View 3D Structure Click here
Q9LHS0 View 3D Structure Click here
Q9LQW4 View 3D Structure Click here
Q9LVD8 View 3D Structure Click here
Q9P6L5 View 3D Structure Click here
Q9SA65 View 3D Structure Click here
Q9SHV5 View 3D Structure Click here
Q9SHV5 View 3D Structure Click here
Q9VI75 View 3D Structure Click here
Q9ZVN6 View 3D Structure Click here