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6  structures 51  species 0  interactions 154  sequences 15  architectures

Family: Flo11 (PF10182)

Summary: Flo11 domain

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Flo11 domain Provide feedback

This presumed domain is found at the N-terminus of the S. cerevisiae Flo11 protein. Flo11 is required for diploid pseudohyphal formation and haploid invasive growth. It belongs to a family of proteins involved in invasive growth, cell-cell adhesion, and mating, many of which can substitute for each other under abnormal conditions [4].

Literature references

  1. Lambrechts MG, Bauer FF, Marmur J, Pretorius IS; , Proc Natl Acad Sci U S A. 1996;93:8419-8424.: Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast. PUBMED:8710886 EPMC:8710886

  2. Lo WS, Dranginis AM; , J Bacteriol. 1996;178:7144-7151.: FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin. PUBMED:8955395 EPMC:8955395

  3. Lo WS, Dranginis AM; , Mol Biol Cell. 1998;9:161-171.: The cell surface flocculin Flo11 is required for pseudohyphae formation and invasion by Saccharomyces cerevisiae. PUBMED:9436998 EPMC:9436998

  4. Guo B, Styles CA, Feng Q, Fink GR; , Proc Natl Acad Sci U S A. 2000;97:12158-12163.: A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating. PUBMED:11027318 EPMC:11027318

  5. Linder T, Gustafsson CM; , Fungal Genet Biol. 2007; [Epub ahead of print]: Molecular phylogenetics of ascomycotal adhesins-A novel family of putative cell-surface adhesive proteins in fission yeasts. PUBMED:17870620 EPMC:17870620


This tab holds annotation information from the InterPro database.

InterPro entry IPR018789

Fungal cells need to be able to adhere to their surroundings, be it either abiotic surfaces or living tissues, in order to remain firmly attached to their source of nutrients. Adhesive properties in fungi are conveyed by a group of cell-surface proteins called adhesins (sometimes also referred to as agglutinins or flocculins). Adhesins are generally rich in serine and threonine residues that allow extensive O-linked glycosylation. Some fungal adhesins also carry discernable ligand-binding domains that determine their substrate specificity and allows some degree of sequence classification of the different groups of adhesins.

Flocculin 11 is the major adhesin for controlling filamentous growth, mat, and biofilm formation of bakers's yeast. Although its amino acid sequence shows less similarity with the other flocculins, Flo11 belongs to the flocculin family. However, the N-terminal domain contains the 'Flo11 domain', but not the mannose-binding PA14 domain, which is present in most other flocculins (Flo1, Flo5, Flo9 and Flo10). Flo11 domains are only found within the ascomycetal orders of Saccharomycetales, which include the budding yeasts as well as the Schizosaccharomycetales, i.e. the fission yeast Schizosaccharomyces pombe. In the majority of its family members, the Flo11 domain is located at the N- terminal ends of glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, although some yeasts such as Clavispora lusitaniae or Spathaspora passalidarum have arrays of up to three Flo11 domains within their adhesins. The Flo11 domain is not a mannose-binding module but can make homotypic interactions [ PUBMED:17870620 , PUBMED:22129043 , PUBMED:25960408 ].

The Flo11 domain consists of some 160 amino acids. It is O-glycosylated and is structurally composed mainly of beta-sheets, which is typical for the members of the flocculin family. The Flo11 domain structure corresponds to a globular, wedge-shaped domain with a complex folding motif that consists of three anti- parallel beta sheets. The sheets I and II form a beta sandwich as core domain, whereas the four-pleated sheet III forms a unique, neck-like subdomain along the lower end of the beta sandwich. The core domain, namely the beta-sandwich formed by the anti-parallel beta sheets I and II, was assigned to the class of fibronectin type III-like domains (FN3) [ PUBMED:25960408 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PLAT (CL0321), which has the following description:

This domain has an 8-stranded sandwich structure.

The clan contains the following 3 members:

ATS3 Flo11 PLAT

Alignments

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(32)
Full
(154)
Representative proteomes UniProt
(294)
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(14)
RP35
(37)
RP55
(107)
RP75
(147)
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  Seed
(32)
Full
(154)
Representative proteomes UniProt
(294)
RP15
(14)
RP35
(37)
RP55
(107)
RP75
(147)
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  Seed
(32)
Full
(154)
Representative proteomes UniProt
(294)
RP15
(14)
RP35
(37)
RP55
(107)
RP75
(147)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_18862 (Release 22.0)
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Linder T , Bateman A
Number in seed: 32
Number in full: 154
Average length of the domain: 144.70 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 18.03 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.0 25.3
Noise cut-off 24.6 24.9
Model length: 150
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Flo11 domain has been found. There are 6 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
P08640 View 3D Structure Click here
Q12140 View 3D Structure Click here
Q9URU4 View 3D Structure Click here