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31  structures 926  species 0  interactions 11060  sequences 186  architectures

Family: O-FucT (PF10250)

Summary: GDP-fucose protein O-fucosyltransferase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

GDP-fucose protein O-fucosyltransferase Provide feedback

This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese [1].

Literature references

  1. Wang Y, Shao L, Shi S, Harris RJ, Spellman MW, Stanley P, Haltiwanger RS; , J Biol Chem. 2001;276:40338-40345.: Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase. PUBMED:11524432 EPMC:11524432


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019378

This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and Caenorhabditis elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese [ PUBMED:11524432 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(155)
Full
(11060)
Representative proteomes UniProt
(17952)
RP15
(1478)
RP35
(5744)
RP55
(9308)
RP75
(12072)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(155)
Full
(11060)
Representative proteomes UniProt
(17952)
RP15
(1478)
RP35
(5744)
RP55
(9308)
RP75
(12072)
Alignment:
Format:
Order:
Sequence:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(155)
Full
(11060)
Representative proteomes UniProt
(17952)
RP15
(1478)
RP35
(5744)
RP55
(9308)
RP75
(12072)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: KOGs (KOG3849)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: KOGs, Finn RD , Coggill P
Number in seed: 155
Number in full: 11060
Average length of the domain: 294.00 aa
Average identity of full alignment: 24 %
Average coverage of the sequence by the domain: 61.54 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 31.5 31.5
Trusted cut-off 31.5 31.5
Noise cut-off 31.4 31.4
Model length: 327
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the O-FucT domain has been found. There are 31 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0N7KE27 View 3D Structure Click here
A0A0P0UYI7 View 3D Structure Click here
A0A0P0VER4 View 3D Structure Click here
A0A0P0WDP9 View 3D Structure Click here
A0A0P0WU21 View 3D Structure Click here
A0A0P0WWZ4 View 3D Structure Click here
A0A0P0X084 View 3D Structure Click here
A0A0P0X9D6 View 3D Structure Click here
A0A0P0XI64 View 3D Structure Click here
A0A0P0XZJ0 View 3D Structure Click here
A0A0R0F2N0 View 3D Structure Click here
A0A0R0FB65 View 3D Structure Click here
A0A0R0GPD9 View 3D Structure Click here
A0A0R0H6D3 View 3D Structure Click here
A0A0R0JH88 View 3D Structure Click here
A0A0R0JRK1 View 3D Structure Click here
A0A0R0K6N2 View 3D Structure Click here
A0A0R0LEI6 View 3D Structure Click here
A0A1D6E3A0 View 3D Structure Click here
A0A1D6E411 View 3D Structure Click here
A0A1D6EHF9 View 3D Structure Click here
A0A1D6EZ98 View 3D Structure Click here
A0A1D6FES0 View 3D Structure Click here
A0A1D6FGJ2 View 3D Structure Click here
A0A1D6FP86 View 3D Structure Click here
A0A1D6FR54 View 3D Structure Click here
A0A1D6FRM3 View 3D Structure Click here
A0A1D6G8B2 View 3D Structure Click here
A0A1D6H165 View 3D Structure Click here
A0A1D6H2V1 View 3D Structure Click here
A0A1D6HFC2 View 3D Structure Click here
A0A1D6HJ27 View 3D Structure Click here
A0A1D6HQ11 View 3D Structure Click here
A0A1D6I472 View 3D Structure Click here
A0A1D6I5C8 View 3D Structure Click here
A0A1D6I5C8 View 3D Structure Click here
A0A1D6I710 View 3D Structure Click here
A0A1D6IB51 View 3D Structure Click here
A0A1D6IKL7 View 3D Structure Click here
A0A1D6IU55 View 3D Structure Click here
A0A1D6JQ16 View 3D Structure Click here
A0A1D6K1P7 View 3D Structure Click here
A0A1D6KKI1 View 3D Structure Click here
A0A1D6KNC6 View 3D Structure Click here
A0A1D6L5G7 View 3D Structure Click here
A0A1D6LVH0 View 3D Structure Click here
A0A1D6LW10 View 3D Structure Click here
A0A1D6M4X4 View 3D Structure Click here
A0A1D6M5T8 View 3D Structure Click here
A0A1D6MDQ0 View 3D Structure Click here
A0A1D6MDQ0 View 3D Structure Click here
A0A1D6MKN8 View 3D Structure Click here
A0A1D6MYH9 View 3D Structure Click here
A0A1D6NRC1 View 3D Structure Click here
A0A1D6NYT6 View 3D Structure Click here
A0A1D6PT98 View 3D Structure Click here
A0A1D6PT98 View 3D Structure Click here
A0A1D6Q1T8 View 3D Structure Click here
A0A368UGR1 View 3D Structure Click here
B0S641 View 3D Structure Click here
B4F8Q9 View 3D Structure Click here
B4FCJ5 View 3D Structure Click here
B4FG68 View 3D Structure Click here
B4FW98 View 3D Structure Click here
B6TA92 View 3D Structure Click here
B7ZWR7 View 3D Structure Click here
B8A0U6 View 3D Structure Click here
C0HEY8 View 3D Structure Click here
C0HFA2 View 3D Structure Click here
C0HIR6 View 3D Structure Click here
C0P5Z2 View 3D Structure Click here
C0P8J1 View 3D Structure Click here
C0PCP2 View 3D Structure Click here
C4J398 View 3D Structure Click here
C6TGB8 View 3D Structure Click here
C7J6Q8 View 3D Structure Click here
D3ZUN5 View 3D Structure Click here
F1R676 View 3D Structure Click here
F4HSU3 View 3D Structure Click here
F4HXW9 View 3D Structure Click here
F4HYR4 View 3D Structure Click here
F4HZX7 View 3D Structure Click here
F4IAL1 View 3D Structure Click here
F4J2C8 View 3D Structure Click here
F4KF16 View 3D Structure Click here
I1JE78 View 3D Structure Click here
I1JEK4 View 3D Structure Click here
I1JJV7 View 3D Structure Click here
I1JSW4 View 3D Structure Click here
I1JV62 View 3D Structure Click here
I1JWL6 View 3D Structure Click here
I1JY27 View 3D Structure Click here
I1JYI9 View 3D Structure Click here
I1K160 View 3D Structure Click here
I1K9Q1 View 3D Structure Click here
I1KFC0 View 3D Structure Click here
I1KFZ5 View 3D Structure Click here
I1KG57 View 3D Structure Click here
I1KH29 View 3D Structure Click here
I1KHK1 View 3D Structure Click here
I1KM63 View 3D Structure Click here
I1KMF7 View 3D Structure Click here
I1KSC1 View 3D Structure Click here
I1KTJ3 View 3D Structure Click here
I1KWK9 View 3D Structure Click here
I1KWL1 View 3D Structure Click here
I1KZS3 View 3D Structure Click here
I1L1F5 View 3D Structure Click here
I1L4R4 View 3D Structure Click here
I1LNG0 View 3D Structure Click here
I1LRR3 View 3D Structure Click here
I1LVF8 View 3D Structure Click here
I1LWT4 View 3D Structure Click here
I1LY54 View 3D Structure Click here
I1MC85 View 3D Structure Click here
I1MFZ7 View 3D Structure Click here
I1MH51 View 3D Structure Click here
I1MHF5 View 3D Structure Click here
I1MJK8 View 3D Structure Click here
I1MK83 View 3D Structure Click here
I1MSB0 View 3D Structure Click here
I1MV20 View 3D Structure Click here
I1N4S3 View 3D Structure Click here
I1N4S5 View 3D Structure Click here
I1ND98 View 3D Structure Click here
K7K1D6 View 3D Structure Click here
K7K1X8 View 3D Structure Click here
K7K291 View 3D Structure Click here
K7K2Z8 View 3D Structure Click here
K7K347 View 3D Structure Click here
K7K8R3 View 3D Structure Click here
K7K9U6 View 3D Structure Click here
K7KAP5 View 3D Structure Click here
K7KDI6 View 3D Structure Click here
K7KMB5 View 3D Structure Click here
K7KSJ6 View 3D Structure Click here
K7KU98 View 3D Structure Click here
K7KUW2 View 3D Structure Click here
K7KV76 View 3D Structure Click here
K7KWA1 View 3D Structure Click here
K7L3Y4 View 3D Structure Click here
K7L7X0 View 3D Structure Click here
K7LBD1 View 3D Structure Click here
K7LC93 View 3D Structure Click here
K7LC94 View 3D Structure Click here
K7LMT2 View 3D Structure Click here
K7LX37 View 3D Structure Click here
K7M1A2 View 3D Structure Click here
K7M429 View 3D Structure Click here
K7M454 View 3D Structure Click here
K7M579 View 3D Structure Click here
K7M7F1 View 3D Structure Click here
K7M7Q4 View 3D Structure Click here
K7MAC8 View 3D Structure Click here
K7MJC8 View 3D Structure Click here
K7MPD4 View 3D Structure Click here
K7MWD1 View 3D Structure Click here
K7N0W5 View 3D Structure Click here
K7N144 View 3D Structure Click here
K7W2X3 View 3D Structure Click here
K7W382 View 3D Structure Click here
K7W382 View 3D Structure Click here
O64884 View 3D Structure Click here
Q0DHQ1 View 3D Structure Click here
Q0DL00 View 3D Structure Click here
Q0INN2 View 3D Structure Click here
Q0J0R2 View 3D Structure Click here
Q0JB77 View 3D Structure Click here
Q0JHU9 View 3D Structure Click here
Q0WPA5 View 3D Structure Click here
Q0WUZ5 View 3D Structure Click here
Q10KX9 View 3D Structure Click here
Q10M06 View 3D Structure Click here
Q18014 View 3D Structure Click here
Q2QWM8 View 3D Structure Click here
Q2QX12 View 3D Structure Click here
Q2R4A8 View 3D Structure Click here
Q4V398 View 3D Structure Click here
Q501D6 View 3D Structure Click here
Q5VNG4 View 3D Structure Click here
Q5Z5W7 View 3D Structure Click here
Q67UT6 View 3D Structure Click here
Q6E279 View 3D Structure Click here
Q6ESK1 View 3D Structure Click here
Q6ETI4 View 3D Structure Click here
Q6EV70 View 3D Structure Click here
Q6H6D5 View 3D Structure Click here
Q6NQ51 View 3D Structure Click here
Q6Z341 View 3D Structure Click here
Q6ZD37 View 3D Structure Click here
Q6ZL29 View 3D Structure Click here
Q7Y030 View 3D Structure Click here
Q84WU0 View 3D Structure Click here
Q8GUM0 View 3D Structure Click here
Q8GZ81 View 3D Structure Click here
Q8H1E6 View 3D Structure Click here
Q8I360 View 3D Structure Click here
Q8L465 View 3D Structure Click here
Q8LNP3 View 3D Structure Click here
Q8LPF8 View 3D Structure Click here
Q8RY81 View 3D Structure Click here
Q8S7V6 View 3D Structure Click here
Q8VHI3 View 3D Structure Click here
Q8W486 View 3D Structure Click here
Q8WR51 View 3D Structure Click here
Q91ZW2 View 3D Structure Click here
Q93ZR8 View 3D Structure Click here
Q949U4 View 3D Structure Click here
Q94BY4 View 3D Structure Click here
Q9FK30 View 3D Structure Click here
Q9FMW3 View 3D Structure Click here
Q9H488 View 3D Structure Click here
Q9LE45 View 3D Structure Click here
Q9LFC6 View 3D Structure Click here
Q9LIN9 View 3D Structure Click here
Q9LU40 View 3D Structure Click here
Q9M393 View 3D Structure Click here
Q9MA87 View 3D Structure Click here
Q9SFC4 View 3D Structure Click here
Q9SH89 View 3D Structure Click here
Q9SVE6 View 3D Structure Click here
Q9V6X7 View 3D Structure Click here
Q9W589 View 3D Structure Click here
Q9Y2G5 View 3D Structure Click here
Q9ZVF7 View 3D Structure Click here